核体

Results NR4A1 was mainly expressed in the cytosol of ova...

核受体NR4A1蛋白的表达与卵泡的发育成熟及黄体的萎缩有明显的相关性。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

According to the experimental results of steady-state spectra and time-resolved fluorescence spectra studied, parallel paths of rod-to-core energy transfer in phycobilisomes have firstly been found, with APCⅠ and APC B being the final acceptors, respectively.

根据稳态光谱和时间分辨荧光光谱实验结果，首次提出了C-PC与APCⅠ及C-PC与APCB的能量传递途径，发现了藻胆体中杆到核的能量传递的并行路径。

The parallelepiped classifier,minimum distance classifier,Maximum Likelihood Classifier,Neural network and,especially,the newly developed Support Vector Machines were assessed in the object-based image analysis of VHSR data. The impacts of kernel configuration on the performance of the SVM and of the selection of training data of the four classifiers were also evaluated.

对比研究了平行六面体、最近邻分类法、最大似然法、神经网络等经典分类算法以及近年来新发展的支持向量机分类算法在基于分割对象的高分辨率遥感图像分类中的性能，详细分析了不同内积核函数对于支持向量机分类的影响。

And at 600℃,in addition to needle carbide,there was pearlite.

在500℃时效处理时，沿晶界形核并向晶内生长的针状碳化物与母相奥氏体之间的位向关系和在晶内析出的针状碳化物的不同；以及在晶内析出的针状碳化物可以交叉生长。

ABSTRACT Heliothis armigera nuclear polyhedrosis virus , due to its special pestiferous effect on Heliothis armigera and being inno...

棉铃虫核型多角体病毒(Heliothis armigera Nuleopolyhedrovirus，HaNPV)是棉铃虫的天然病原体，对棉铃虫有特异的致死效应，不危害人畜及环境，是进行棉铃虫生物防治的好材料。

The plastid transformation of plant has become an alternative to genetic engineering besides nuclear genome.

植物质体转化开辟了除核基因组之外的另一条遗传转化途径，10几年来取得了很大的进展。

However, plastids only have a semi-autonomous transcription and translation machinery. The transcription of endogenous plastid genes is largely dependent on nuclear-encoded transcription factors.

然而， 质体仅具有半自主性转录和翻译机制，其内源基因的转录要依赖于核基因编码的转录因子。

Germinal vesicle breakdown and the first polar body (PB1) extrusion were observed in all groups.

观察各组生发卵泡破裂和第一极体(PB1)的形成，出现PB1的卵母细胞视为核成熟。

Spodoptera litura ; Nuclear polyhedrosis virus ; Disease prevalence ; Time dosage mortality model.

斜纹夜蛾；核多角体病毒；疾病流行学；时间剂量死亡率模型

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。