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The apoptotic effects of LPS are detected by various skills such as fluorescence staining, TUNEL reaction, DNA agarose gel electrophoresis, electron microscopy and fluorescence activated cell sorting. Our results demonstrated that fish lymphocytes would be apoptotic under different LPS concentrations(above 10μg/ml) and different time(above 6h) inducing, which is characterized by condensation and margination of chromatin, the formation of apoptotic bodies.

结果显示,在10μg/ml以上浓度的内毒素作用18后或以100μg/ml浓度的LPS作用6h以上,淋巴细胞即可出现明显的凋亡形态学变化,表现为染色质凝集和周边化,核断裂,形成凋亡小体,DNA发生特异降解产生典型的"梯状"电泳图谱,呈现TUNEL阳性标记等,并通过流式细胞术证实这种凋亡具有时问和剂量的依赖性。

BACKGROUND: Studies demonstrate that there are some effects of oral masticatory function on the fracture resistance ability of the fiber post-core prosthesis.

摘要背景:研究表明口腔的日常咀嚼功能会对纤维桩核修复体的抗折性能产生影响。

Aluminosilicate mesoporous molecular sieves are synthesized with natural ore and microcline as main raw materials,and the precursors of 13X zeolite as crystal source,by integrating the synthetic procedures of mesoporous MCM-41 and microporous 13X zeolite.

以天然矿物微斜长石为主要原料,13X沸石前驱体为晶核剂,结合介孔分子筛MCM-41与13X沸石合成工艺,合成了孔壁中含有13X沸石基本结构单元的铝硅酸盐介孔分子筛。

Aluminosilicate mesoporous sieve s with 13X zeolite units in the pore wall were synthesized with a natural ore,microcline,as the main raw material,and the precursors of 13X zeolite as the crystal source,by integrating the procedures for MCM-41 and 13X zeolite synthesis.

以天然矿物微斜长石为主要原料,以13X沸石前驱体为晶核剂,结合介孔分子筛 MCM-41与13X沸石合成工艺,合成了孔壁中含有13X沸石基本结构单元的铝硅酸盐介孔分子筛。

Aluminosilicate mesoporous sieves with 13X zeolite units in the pore wall were synthesized with a natural ore, microcline ,as the main raw material,and the precursors of 13X zeolite as the crystal source,by integrating the procedures for MCM-41 and 13X zeolite synthesis.

以天然矿物微斜长石为主要原料,以13X沸石前驱体为晶核剂,结合介孔分子筛MCM-41与13X沸石合成工艺,合成了孔壁中含有13X沸石基本结构单元的铝硅酸盐介孔分子筛。

Series of octahedral core-shell emulsion microcrystal with different distribution of iodide ions was prepared by double-jet method.

本文应用双注法制备了一系列碘分布不同的八面体核壳乳剂。

METHODS Microdissection technique with micromanipulator under microscope, as well as subsequent DNA extraction and polymerase chain reaction amplification methods, was employed.

通过光学显微镜直视下用显微操纵系统进行显微切割技术结合DNA提取和PCR方法,对在组织切片上获取病毒核内包涵体的来源进行分子生物学鉴定。

The effect of misch metal and yttrium on the ageing behavior of AZ91 magnesium alloy was investigated.Aluminum compounds of rare earth and yttrium with high melting point were crystallized fiirstly from the melt alloy during the solidifying process and pushed to the crystalllizing face of α phase to inhibit its growing,or became the nucleation of the matrix.

在AZ91镁合金中添加稀土和钇之后,高熔点的铝稀土化合物和铝钇化合物在凝固过程中首先结晶析出,并成为基体相的形核中心,或者被推移到初生α相结晶前沿,阻碍α相的生长,使其铸态组织得到细化。

RESULTS AND CONCLUSION: After purification, the cell body of SGCs from cochlear modiolus was ellipse or triangle, with slender processes in cytoplasm. Nuclei were positive for Nuen.

结果与结论:蜗轴组织细胞纯化后,胞体呈椭圆形或三角形,有细长的突起,Nuen染色胞核呈棕黄色阳性反应,β3-Tubulin染色细胞胞浆与轴突均呈棕黄色阳性反应。

RESULTS AND CONCLUSION: After purification, the cell body of SGCs from cochlear modiolus was ellipse or triangle, with slender processes in cytoplasm. Nuclei were positive for Nuen. Cytoplasm and axon were positive for β3-Tubulin.

结果与结论:蜗轴组织细胞纯化后,胞体呈椭圆形或三角形,有细长的突起,Nuen染色胞核呈棕黄色阳性反应,β3-Tubulin染色细胞胞浆与轴突均呈棕黄色阳性反应。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。