核体

Some cases without PGRN mutations also have ubiquitinated neuronal intranuclear inclusions.

一些患者没有PGRN突变，但也有泛素化的神经元核内包涵体。

Small cell carcinomas usually do not show large prominent nucleoli, whereas melanomas often do, accompanied by occasional intranuclear pseudoinclusions.

小细胞癌常无明显的大核仁，偶尔伴有核内假包涵体。

As a result, not only 3-ene isomer of cephem nucleus producing was avoided, but also the cost was reduced.

这样既降低了成本，又避免了碱性反应条件下头孢菌素母核3-烯异构体的生成。

In contract, the tumor tissue of control group kept morphology, tumor nodules composed of undifferentiated carcinoma, and some karyokinesis could be seen, some spotty necrosis were found occasionally in where the structure among tumor cells were loose associated very slight inflammatory response; At the 21 days after injected AdCMVIL-12, the tumor boundary of AdCMVIL-12-Ⅰ group were clear and felt hard by touch, section showed grey.

同期的对照组绝大多数肿瘤细胞形态完好，排列紧密，由大量未分化的肿瘤细胞组成并可见多个核分裂像，偶尔可见散在的点状坏死灶，瘤细胞之间结构松散，炎症反应很轻；AdCMVIL-12注射后第21天时，AdCMVIL-12-Ⅰ组的肿瘤组织经手摸感觉瘤体境界清楚、发硬，切面呈灰白色。

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

METHODS: Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA31.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩，管壁增厚呈空虚网状，生精细胞层次减少，间质细胞增生，但仍见有精子生成；高剂量组可见到精子呈异型性改变，细胞核浓缩深染，且随着摄入时间延长改变愈趋明显；F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩，间质增生明显，20个月时肾小球萎缩纤维化；F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生，淋巴母细胞增生活跃，20个月时脾小体减少，生发中心稀疏；F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润，晚期骨髓有核细胞减少，脂肪细胞增加。

A stable nucleus is an aggregate of the macromolecule.

一个稳定的晶核是这些生物大分子的聚积体。

Interval observations under thefluorescent microscop were performed with excition waves of 470—490 nm.The results displayed that GFP genes were expressed effectively in E.octocarinatus, and GFP was distributed unifimly around macronuclearand diffused to the entire cell gradually. The chromosomes can be kept inthe macronucleus for 90h.

用优化的脂质体转化方法将含有人工染色体的重组质粒pBTub-tel2转化到处于有性生殖分裂间期阶段的游仆虫细胞中，结果表明，GFP基因在游仆虫细胞中得以高效表达，36-48小时绿色荧光均匀分布在细胞核周围，逐渐扩散到整个细胞，染色体能够在大核中保持约90小时。

By using TUNEL, FCM, EM and color image analysis, PCD was studied during postnatal brain development in rats. TUNEL revealed chromatin condensation, which marginate to the edge of nuclear and sometimes form crescentic caps or ring. Occasionally apoptotic bodies were observed.

一、应用TUNEL、FCM、电镜，结合图像分析对大鼠生后脑发育过程中PCD观察发现：在TUNEL染色上PCD细胞表现为染色质凝聚成块、移向核边缘、有时形成环状和半月状，偶尔可见凋亡小体等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。