核体

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

The sloping plate can provide sufficiently the under-cooling for nucleation and lay a foundation for heterogeneous nucleation to form lots of nuclei of crystal internally and on the sloping plate surface, thus causing the melt to nucleates eruptively. Under the action of both metal flow and vibration, the nuclei formed on the sloping plate surface can move freely and disperse into the melt, and they are able to maintain the direct growth in spherical shape in the homogeneous composition and temperature fields.

倾斜板为形核提供足够大的过冷度和异质形核基础，大量晶核在斜板表面和内部形成，使熔体爆发形核，在流动和振动作用下斜板表面形成的晶核游离，弥散到熔体内部，晶核在均匀的成分场与温度场条件下，可以保持直接球状生长。

Both the new peniculus 1 and the old haplokinety separated at the telophase.The two germinal rows germinal rowsfor both the daughter cells appeared almost at the same time;(2) The macronucleus became shorter and thicker, finally oblate during the division of the cell. Then the macronucleus restored its original shape, band-like form. The micronucleus were divided into two before the macronucleus division;(3) The original scopula and stalk contributed to the old cell. The new cell formed scopula gradually after the cell division.

同时，新仔虫的第一咽膜(P′1)也开始由老单毛基索复制，并在细胞分裂后期与老结构分离；(2)大核在虫体分裂过程中由长带状逐渐缩短变粗至扁圆形，于虫体即将分开时迅速拉长，然后分裂为二个新大核；小核分裂先于大核，在两仔虫口纤毛器即将分开时完成；(3)原帚胚及柄归属老仔虫，新仔虫的帚胚在虫体分裂后逐渐形成，柄内肌丝则在柄鞘形成后逐渐长出。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。

An analysis revealed very significant positive correlation between trophozoite area and nucleus area (r=0.54, P .01), trophozoite volume and nucleus volume(r=0.47, P .01), and negative correlation between trophozoite area and trophozoite roundness (r=-0.29, P .01), no significant correlation between nucleus roundness and trophozoite roundness.

相关分析显示滋养体与核的面积(r=0 。5 4 ，P 。0 1)、滋养体与核体积(r=0 。4 7，P 。0 1)相关性有显著性意义，滋养体面积和核圆度呈负相关(r=- 0 。2 9，P 。0 1)，滋养体圆度和核圆度之间无相关性。

It is the first time that these phenomena were observed: Two nuclei are surrounded by plasma and locate at the central of the female gametophyte and the others nuclei are positioned at the fringe of the female gametophyte; Before the pollen tube enters the female gametophyte. the nuclei in chalazal end begin splitting of plasma and form multinuclear cell.4. The pollens of Gnetumc are spherical or applanate with single aperture. The ornamentation of exine is spine. The basis part of spine is lenience and the top part of spine is tip or obtuse sphere.

首次在买麻藤属植物的雌配子体中观察到2个游离核位于配子体的中央位置，且被一团原生质所包围的，其余的游离核位于边缘的现象以及花粉管进入雌配子体前，合点端的核已经发生胞质分裂形成多核细胞的现象 4、买麻藤的花粉近球形或扁平型，有单萌发孔，外壁表面具小刺状纹饰，小刺基部宽大，末端尖或钝圆。

The results showed that orexin-like immunoreactiveneurons were observed in the medial preoptic nucleus,periventricular nucleus,paraventricular nucleus,supraoptic nucleus,dorsomedial nucleus,perifornical nucleus,mammillary nucleus,anterior area,lateral area and posterior area of the hypothalamus,with majority in the lateral area,supraoptic nucleus and mammillary nucleus and with minority in t...

结果表明，在猪下丘脑内，开胃素A免疫阳性神经元分布于下丘脑的视前内侧区、室周核、室旁核、视上核、背内侧核、穹隆周核、乳头体核、前区、外侧区和后区等部位，以下丘脑外侧区、乳头体核和视上核出现的免疫阳性神经元最多，以室周核的最少。这一结果与在其他动物上获得的基本相似。

After HRP application to the rostral area (n=10), quite a number of labelled cells were distributed at the ventrolateral part of the nucleus of trapezoid body, the lateroventral periolivary nucleus and the retrotrapezoid nucleus. After HRP application to the caudal area (n=10), HRP labelled cells were mainly located at the lateral reticular nucleus and the ventral part of at the lateral paragigantocellular nucleus LPGi. After HRP application to the intermediate area (n=6), only 8HRP labelled cells were observed at the in 6rats. After HRP application to the control area (n=4), only 6HRP labelled cells were found at the rostral ventrolateral reticular nucleus.

大鼠延髓腹外侧表面的头端化学感受区表面敷贴辣根过氧化物酶后，在延髓斜方体核、外周橄榄腹外侧核和斜方体后核均有HRP标记细胞存在；于尾端化学感受区表面敷贴HRP后，在延髓的巨细胞旁外侧核、外侧网状核和舌下神经核有HRP标记细胞存在；于中间区表面敷贴HRP后，在6只大鼠的延髓内部区域仅观察到8个HRP标记细胞；于对照区敷贴HRP后，在4只大鼠的延髓内部区域仅观察到6个HRP标记细胞，散在地分布在头端腹外侧网状核。

After HRP application, HRP labelled cells were distributed in the nucleus of trapezoid body , lateroventral periolivary nucleus , retrotrapezoid nucleus , lateral paragigantocellular nucleus and lateral reticular nucleus . The findings demonstrated the existence of neural structural relations between the chemosensitve areas and the Tz, LVPO, NRT, LPGi and LRt, and revealed that these nuclei might participate in the mechanism of central chemical regulation on respiration.

观察到用浸有HRP的滤纸局部敷贴于延髓腹外侧表面化学感受区后，在延髓斜方体核、外周橄榄腹外侧核、斜方体后核、巨细胞旁外侧核和外侧网状核等核团找到HRP标记的细胞，表明上述延髓内部核团与腹外侧表面化学感受区之间存在神经结构联系，提示延髓腹外侧表面化学感受区对H〓等物质的化学感受机制可能与上述核团神经元伸向该区的突起有关。

HVc received projections from medial nucleus magnocellularis of the anterior neostriatum,nucleus interfacialis of midneostriatum,telencephalie auditory nucleus-field L,nucleus uvaeformis of the thalamus,and locus ceruleus of the pons.

投射到古纹状体粗核和嗅叶X区，HVc接受新纹状体前部大细胞核内侧部、新纹状体中部界面核、端脑听核一L区、丘脑葡萄形核及脑桥蓝斑核的传入投射。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。