核体

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

Endomitosis The duplication of chromosomes without division of the nucleus, causing polyploidy and leading to an increase in nuclear and cytoplasmic volume.

核内有丝分裂：在细胞核不分裂的情况下进行染色体复制，产生多倍体并使细胞体积增大的一种有丝分裂形式。

AIM: To study the function of hepatitis C virus internal ribosome entry site, and to construct biscistronic vector.

目的： 研究丙型肝炎病毒核糖体插入位点启动翻译蛋白质功能，构建双顺反子真核表达载体。

In contrast to xylem components , sieve tube elements have nonlignified cell walls , and a living enucleate without a nucleus protoplast .

与木质部组分不同，筛管分子具有未木质化的细胞壁，没有核但有活的原生质体，因此是活细胞。

The results show that a large number of fine crystal grain are formed on the surface of a crystal nucleus eradiation equipment with cooling system while the vibrating bar of the equipment is inserted into 30% NH4Cl aqueous solution.

结果发现：当一种带有冷却结构和高频振动的晶核发射器棒体插入氯化铵溶液时，在棒体表面将迅速形成大量的细小晶粒。

The concept of chromatin and chemic compose; chromatin proteins; the essential about the structure of nucleosome; euchromatin and heterochromatin.

二 染色质的概念及化学组成；染色质蛋白质；核小体结构要点；染色质包装的结构模型；常染色质和异染色质。

These DSs were first observed at 9 weeks in the distal part of ascending fibers that consist of gracile fasciculus and cerebellospinal-gracile nucleus and white matter of cerebellum. They extend along medulla oblongate to medulla cervix、medulla thorax、medulla lumbalis in accordance with dying-back type degeneration. The corticospinal tract were affected at 18 weeks,and thalamus、the olfactory pathway、the visal pathway、the auditory pathway were affected at 32 weeks.

这种DS从9周龄开始，首先在构成薄束路和脊髓小脑后路的上行纤维的远端区域-薄束核区和小脑白质出现，逐渐按照延髓、颈髓、胸髓、腰髓的顺序，向细胞体方向逆行性地进展。18周龄时波及到锥体路，32周龄时进一步波及到丘脑和嗅觉、视觉、听觉传导路。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

About 13 months after pollination, when the female gametophyte contains some 2000 free nuclei, cell wall formation begins.

在授粉后大约 13个月，当女性的配偶体包含一些 2000个自由的核的时候，细胞壁形成开始。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。