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Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

In this study aldylencdithiomethylene Malonic acid acts as the main coordination agents and compound iron ion have been synthesized by the means of solution, As a result ,the 1D polymer is obtained.

本论文采用常规的溶液合成方法,首先设计合成了一个以含有二硫缩烯酮结构的丙二酸类配体和过渡金属铁离子的单核配合物,再以此单核配合物作为配体设计合成了三个异多核配合物和配位聚合物。

Micrococcal nuclease hypersensitive DNA fragments were either cloned directly, or mapped onto the genome by hybridization with oligo-nucleotide probes on a 244K Agilent array-CGH chip. The array-CGH mapping was confirmed for nuclease sensitivity by PCR after MNase digestion and by Southern blot analysis.

我们利用小球菌核酸酶超敏感的去氧核醣核酸片段直接选殖或是将其作为寡核苷酸探针於安捷伦244k寡核苷酸阵列-比较基因体杂合晶片上,进行基因体定位,并利用聚合酶链索反应和南方墨点法侦测其核酸酶敏感程度,以确认结果之可靠性。

Pseudelatia separata (Lepidoptera: Noctuidae) infected with Pseudelatia separata NPV and Pseudelatia separata GV and coinfected with the two viruses, the structural characters of its prothoracic gland and the ultrastructure of the gland cells were observed. The results showed that pathological changes appeared in the infected PTGs.

观察比较了粘虫核型多角体病毒(Pseudelatia separata NPV,PsNPV)、粘虫颗粒体病毒(Pseudelatia separataGV,PsGV)感染粘虫,以及两种病毒混合感染粘虫后,粘虫前胸腺的形态特征和前胸腺细胞的超微结构。

Involvement of central tegmental tracts produces ipsilateral olivary degeneration; that of dentate nucleus or superior cerebellar peduncle produces contralateral olivary degeneration.

累及背盖中央束导致同侧橄榄体退变,累及齿状核或小脑上脚导致对侧的橄榄体退变。

The results show that the Luzhou Palaeohigh is the favorable region to develop the intraplatfonn oolitic bank during the slow equivalent regression process, moreover, the bank formed was characterized by a large thickness and wide distribution area.

结果表明,在缓慢等效的海退过程中,处于高能沉积界面附近的水下古高地有利于台内鲕滩的形成,继承性的泸州古隆起核部是区内有利的滩体发育区,形成的台内滩体具有一定的厚度规模和较大的分布面积。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株,采用平板测试、温室盆栽及田间试验等方法,筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株,在离体的条件下均表现出不同程度的拮抗作用,其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱,对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用,并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

Anthraea pernyi nucleopolyhedrovirus ; Polyhedral envelope protein gene ; Sequence of amino acid

柞蚕核型多角体病毒;多角体膜蛋白基因;氨基酸序列

After the first division of the zygote, organelles from male and female gametes appear to be equally incorperated into each cell of the two-celled proembryo.

中央细胞极核周围的细胞质中有各种细胞器,包括质体、线粒体、粗面内质网和高尔基体。

Methods Fibroblastic stromal cells were isolated from fresh pseudomembrane from 7 patients undergoing revision arthroplasty for aseptic loosening of the hip and cultured in the presence and absence of bone cement (PMMA+BaSO4) particles.

酶消化法从人工假体周围假膜标本中分离FBSCs并作体外培养并加入骨水泥颗粒,检测FBSCs核因子kB受体激动剂配体和肿瘤坏死因子mRNA表达的变化。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。