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Its zinc complex was applied in asymmetric direct aldol reaction of acetone and p-nitrobenzal...

设计合成了一种NOBIN衍生的手性配体,初步研究了该手性配体的双核锌配合物在对硝基苯甲醛和丙酮的不对称直接醇醛缩合反应中的催化应用,20℃和20mol%催化剂量下,获得了32.5%ee和72%收率的产物。

Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接,并接入表达载体pcDNA 3.1中,构成含hMCP-DAF的真核表达质粒(pcDNA-MD3);以脂质体转染猪内皮细胞,人血清处理阳性克隆细胞,鉴定抑制人补体溶破的功能;应用显微注射将线性基因导入猪受精卵;PCR检测导入基因整合率。

In present review, focusing on the low-copy nuclear genes, we summarize the appli-cation of these genes in the phylogenetic reconstruction of allopolyploid plants and bring forward some critical issues in this field.

以低拷贝核基因为主线,概述了其在多倍体植物系统发生中的应用和相关注意事项,并对其在多倍体植物中的表达变化及其分子机制进行了探讨,系统地介绍了国际上相关领域的研究成果和最新动向。

The effect of Mo and Nb platings as well as chemical etching on diamond nucleation and growth on cemented carbide YG6 are studied and discussed thoroughly.

深入研究了镀Mo、镀Nb及化学腐蚀等表面改性方法对YG6硬质合金基体上金刚石形核及生长的影响,分析了这些方法影响金刚石形核的机理。

High transverse momentum partons which come from hard scattering will propagate in the strong interaction medium and interact with matter. These partons are expected to lose their energy in the medium by gluon bremsstrahlung, which is called Jet Quenching.In BNL/RHIC Runl (S_~(1/2)= 130GeV and Run2 (S_(NN~(1/2)= 200GeV, comparing the yield of leading particle in nucleus-nucleus collisions with nucleon-nucleon collision finds that charged and neutral pions are suppressed.

在相对论重离子碰撞中,末态高横动量强子产额的压低被认为是夸克胶子等粒子体已经生成的一个重要信号,在高能核-核碰撞过程中,硬散射发生在碰撞过程的初期,由硬散射过程形成的大横动量部分子将穿越强相互作用物质,这些硬部分子与热密物质发生强相互作用,经过韧致辐射而损失能量,我们把这种现象称为喷注淬火。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示:(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散,但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系,卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准,只有排出第一极体才能作为猪卵母细胞核成熟的标志;(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率,而且可加强卵丘细胞的扩散;(3)在现有实验条件下,在mTCM和mNCSU中添加10%pFF与在mNCSU中添加10%FCS相比可获得较高的猪卵母细胞核成熟率;(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样,但对体外受精后的早期胚胎发育影响不明显;(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

Karyotype formula of Gossypium hirsutum L.was 2n=4s=52=30m+20m (4SAT)+2st (2SAT); and its absolute lengths of chromosomes averaged 3.40μm; arm ratio differed from 1.24 to 4.02,with the mean value of 1.77; three pairs of satellite chromosomes; the ratio of the longest to the shortest chromosomes was 2.36; and the karyotype belonged to 2B in Stebbin's classification.

陆地棉的核型公式为2n=4x=52=30m+20sm(4SAT)+2st(2SAT);染色体绝对长度平均3.40μm;臂比值最大与最小的分别是4.02和1.14,平均1.77;随体3对,分别位于第12、19和26对同源染色体的短臂上;最长与最短染色体的比值为2.36;其核型属于Stebbin的2B类型。

The results showed that when PA1010 was filled with 1.5% n- SiO2, the properties of the coating was best .The coating-substrate adhesion stress was 28.9 MPa . The self-strength of the coating was 69.8 MPa. DSC analysis shown that n- SiO2 could increase the crystallization speed of PA1010 coatings obviously.

结果表明:当复合涂层配比为m(PA1010):m(n- SiO2)=100.0: 1.5时,复合涂层综合性能较佳,涂层自拉伸强度为69.8 MPa,涂层与基体结合强度为28.9 MPa;纳米SiO2有明显的异质成核作用,使复合涂层的过冷度由27.0 ℃下降为24.0 ℃;纳米SiO2能够显著提高涂层的力学性能,有助于提高复合涂层的结晶速率,具有明显的成核作用。

Methods Mammalian expression vectors containing human MGMT gene were tranferred into PBMC in vitro via liposome. Realtime PCR and Western blot were used to detect the expression of MGMT at mRNA and protein levels.

利用脂质体法将含入MGMT基因的真核表达载体导入体外培养的外周血单个核细胞,实时PCR及Western blot检测目的基因mRNA及蛋白水平的表达。

In the study of isospin-dependent nucleon-nucleon elastic crosssections in nuclear medium we find the medium correction of nucleon-nucleon scatteringcross sections is also isospin dependent,〓 depends on the baryon density weakly and 〓 depends on the baryon density significantly.

用QHD-Ⅱ模型研究了介质两体截面的同位旋相关性,研究结果表明截面的介质修正也是同位旋依赖的:中子-质子介质截面对核密度的依赖很弱,而中子-中子的介质截面对核密度的依赖很强。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。