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AIM: To observe whether the behavior of polymorphonuclear leukocyte adhesion to the endothelial cell after trauma-hemorrhage and resuscitation is different between genders by means of intravital microscopy, and determine the expression of PMN adhesion molecule CD11b.

朱志宏,沈洪,李冰,田万管目的:在体实时观察创伤失血性休克复苏后微循环多形核白细胞在毛细血管后微静脉内皮的附壁是否存在性别差异,并测定多形核白细胞黏附因子CD11b的表达。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔,然后一边缓慢地将注射针回拔至卵周隙中,一边逐渐增加持卵针中的负压,直至极体与目标核质被完整吸入持卵针中而完成去核,最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

The ultramicroscopic structure proved that the size of kinetoplast, nucleus, nucleolus and electron dense of nuclear membrane of the parasite were large obviousely than that of the control.

超微结构显示,HGPRT缺陷株的动基体、核和核仁比对照组明显增大,核膜电子密度区也明显增宽。

The staining of brush cells of experimental group showed nuclear shrinkage, margination, nuclear fragmentation and formation of apoptotic bodies, and the characteristic ladder band on gel electrophoresis. NO changes were observed in control group.

实验组细胞刷片染色显示有明显的细胞核固缩、核周边染色质加深、核断裂、凋亡小体现象,细胞DNA电泳可见梯状条带的凋亡特征,而空白对照组无此现象。

RESULTS: There were many vacuoles and cracks in the cells, with heterochromatin margination, nuclear pycnosis and nuclear fragmentation. The high intensity pulsed electric fields caused damage in K562 cells, and the apoptotic percentage went up with the increase in frequency and intensity of PEF.

结果:经过高电压脉冲电场处理后的K562细胞,胞内出现大量空泡及裂隙,核内染色质向核周边聚集形成凋亡小体,呈典型细胞凋亡形态学改变;K562癌细胞的凋亡比例随着脉冲个数和电场强度的增加而增加。

The system of dopaminergic neurons that projects from the tegmentum of the mesencephalon to various telencephalic sites, including frontal cortex, striatum

多巴胺能核的系统从中脑的大脑脚盖发射到不同的终脑位置,包括脑额叶皮质、纹状体、隔膜、扣带脑皮层、杏仁孔和阿肯柏氏核。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物,从猪卵巢上获得的卵母细胞数量虽然较多,但成熟度比较低;由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内,跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应;如果卵胞质成熟或活化程度不够,加之圆形未成熟精细胞成熟度比较差等原因,很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足,引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等,使ROSI显微受精失败。

The phenomenon of polyploidy and aneuploidy exists in the Genus, in which there are polyploid complex.

对19个分类群作了核型观察,其中16个分类群作了组型分析,结果表明各分类群间核型差异显著,该属普遍存在着多倍性和非整倍性现象,并有多倍体复合体类群存在。

In a dorsal striatum region known as the putamen, which plays an important role in habits and is also inoled with attention, dopamine transporter leels did not differ between the two groups.

而在纹状体背侧的壳核中,两组之间多巴胺转运蛋白的水平差别不大,壳核在习性形成与注意力的集中的过程中发挥着重要作用。

Glutamate and GABA in the substantia nigra may affect iron metabolism through DMT1-IRE and HP in the caudate putamen nucleus.

黑质的谷氨酸和GABA可能通过影响尾壳核DMT1-IRE和HP的表达影响纹状体尾壳核的铁代谢。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。