核体

Cephalotaxine ester of 2-oxo-6-methylheptanoic acid was treated with lithium diisopropylamide and Me O-(1-methoxyisopropyl)glycolate to give a mixture of homoisoharringtonine and its stereoisomers with 56% yield. The four stereoisomers separated by TLC showed identical mass spectra and almost superimposed IR but with different 1H NMR spectra. The configuration of the 2'-C and 3'-C of the four isomers were postulated based upon their 1H NMR spectra as compared with the 1H NMR spectra of isoharringtonine and its stereoisomers.

2-氧代-6-甲基庚酰基三尖杉碱(3)与0-(1-甲氧基异丙基)羟基乙酸甲酯(4)在强碱性试剂二异丙胺锂存在下，起亲核加成反应，反应中间体5在室温用酸性丙酮水解，得到一种新的三尖杉酯类生物碱一一高异三尖杉酯碱(6c)及其立体异构体(6a、6b、6d)的混合物，产率56%、通过制备薄层层析分得这四个立体异构体，它们的1H NMR和异三尖杉酯碱及其立体异构体的1H NMR类似，推定了它们的绝对构型。

Ti composite oxide could promote the formation of acicular ferrite and microstructure of coarse-grain HAZ of Ti treated steel consists of ferrit in grain boundaries, ferrite side plate and large amount of intragranular acicular ferrite.

复合Ti的氧化物具有促进晶内针状铁素体形核的能力，焊接粗晶区主要由晶界铁素体、少量的侧板条铁素体和大量的晶内针状铁素体组成。

This phenomenon suggests that the interactions between the nucleus and plastids are reciprocal: the development of plastid is controlled by the nuclei, on the other hand, certain nuclear genes by plastids.

这说明细胞核和质体之间的作用是相互的：质体的发育受细胞核的调控，另一方面，某些核基因的表达也受到质体的控制。

In order to assure that the plant can be safely shutdown and to comply with the requirement of 10CFR 50.62, various ATWS prevention mitigation features have been implemented in the design of Lungmen Plant, including Fine Motion Control Rod DriveRun In, Alternate Rod Insertion, Automatic Initiation of Standby Liquid Control System, Reactor Internal Pump Trip/ Runback, Feedwater Flow Runback, and Inhibition of Automatic Depressurization System.

摘要 核四厂属进步型沸水式核能电厂，其采用数位式仪控系统设计，考虑设计上可能存在软体共因失效问题，导致反应器无法顺利急停，产生预期暂态未急停（Anticipated Transient Without Scram， ATWS）事件，为确保反应器安全，并符合10CFR 50.62法规要求，电厂设计上装设有缓抑ATWS事故之软体与硬体设施，包括微调控制棒驱动机构插入、替代性控制棒插入、备用硼液控制系统自动启动、炉内泵跳脱、炉内泵回退、饲水回退与禁止自动释压系统开启等型态。

The result showed that the plant of Maicao No. 9 was tall, with good fecundity, fully filled grain and vigorous growth. The chromosome number of the root cells was 2n=56, through the observation of pollen mother cells meiosis, there were a certain amount of univalents, ring bivalents and bar bivalents in PMCM. Different numbers of lagging chromosomes, chromosome bridges and micronucleus were found in anaphaseⅠ, anaphaseⅡ and tetraspore.

结果表明：麦草9号植株高大，结实性较好，籽粒饱满，生长旺盛；根尖细胞染色体数目为2n=56，对其进行花粉母细胞减数分裂观察，中期Ⅰ具有一定数量的单价体、环状二价体、棒状二价体；后期Ⅰ、后期Ⅱ与四分体时期都出现不同数量的落后染色体、染色体桥、微核等现象。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Morphology and nucleus phase showed that arthrospores are larger than basidiospores in diameter, basidiospores are mononuclear cells, the majority of arthrospores are dikaryocytes and the mononuclear cells are in minority, mycelia are dikaryocytes with typical clamp connection.

显微形态及核相观察表明：节孢子直径略大于担孢子，担孢子为单核细胞，节孢子绝大多数为单核细胞，少数为双核细胞，菌丝体为双核细胞并具有典型的锁状联合结构。

Pitardii,all from the Jinshajiang Valley of Yunnan and Sichuan, were cytologically studied in this work. Among 34 populations of C.reticulata studied here, 21 were found to be tetraploids (2n=60), 11 hexaploids (2n = 90), and the remaining two diploids (2n = 30). Tetraploid was reported in C. reticulata for the first time and its karyomophology was studied.

reticulata及其两个近缘种(怒江山茶C.saluenensis和西南山茶C.pitardii)进行了细胞学研究。34个居群的云南山茶花中，21个居群是四倍体类型(2n=60)，11个居群是六倍体类型(2n=90)，另2个居群为二倍体(2n=30)，云南山茶花的四倍体类型为首次发现，并且进行了核形态研究。

The major clinical manifestation was lymph node enlargement found in all patients. Skin involvement and osteolysis were not frequent. The characteristic finding was leukemic cells with highly indented or lobulated flower-like nuclei in peripheral blood and bone marrow. ATL cells were CD2,CD3,CD4,CD25 positive and CD8 negative. No specific chromosome abnormality or HLA type was found. Seven of 8 patients examined had HTLV-Ⅰ antibody. The HTLV-Ⅰ provirus genome sequence integrated into host cell DNA was amplified by PCR and confirmed by liquid hybridization. All of these results showed that HTLV-Ⅰ was also the etiological agent of ATL in China. One of the 9 cases of ATL was classified as lymphoma type, one as chronic-type, and the rest as acute type.

结果和结论确诊9例ATL，其临床特征主要为淋巴结肿大，皮损及溶骨改变少见，外周血及骨髓出现典型的花瓣状核淋巴细胞，胞体大，核畸形，免疫表型为CD-1、CD+2、CD3+、CD4+、CD-8，还表达CD25等激活标记，染色体核型及HLA分型未发现有规律性的变化。8例ATL中有7例血清HTLV-Ⅰ抗体阳性，应用PCR及液相杂交法可检出整合到宿主细胞DNA中的HTLV-Ⅰ病毒序列，表明在我国HTLV-Ⅰ也是ATL的病因。9例中1例为淋巴瘤型，1例为慢性型，7例为急性型ATL，治疗效果及预后均不良。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。