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ABSTRACT Heliothis armigera nuclear polyhedrosis virus , due to its special pestiferous effect on Heliothis armigera and being innocent to human, livestock and environment, has been a commercial bioinsecticide widely in the world for years.

棉铃虫核型多角体病毒是我国最早注册的杆状病毒杀虫剂,到1999年,约有100,000公顷棉田用棉铃虫核型多角体病毒的生物制剂防治害虫。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要:用冷冻保存的花鲈精子作为异源精子,采用紫外线对精子进行照射使其遗传物质失活,然后与卵子进行"授精",可以刺激犬齿牙鲆鱼卵进行雌核发育,在受精后一定时间采用冷休克处理"授精"卵,抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

Studies on the Photosynthesis of Several Trisomics in Chinese Cabbage Brassica campestris ssp. pekinensis (Rupr. Olsson;3. The karyotype of triplo 6 of indica IR36 trisomic is analysized, and the additional chromosome is inentified.

本研究以籼稻IR36第六号三体(triplo6)为材料,进行了水稻体细胞核型分析,同时鉴定验证了三体材料,并为水稻标准核型图的建立提供了一些依据。

Along the length of the uncoiled chromosomes are beadlike structures called nucleosomes, highly organized aggregations of DNA and histones.

解聚后的染色体呈现一系列核小体相互连接的串珠状结构,核小体是由 DNA 和组蛋白高度有序的结合形成的。

The results indicated that a high volumetric ratio of acicular ferrite in the simulated weld metal can be obtained in case the ratio of manganese and silicon is in a certain range.

结果表明,当Si、Mn比在一定的范围时,焊缝中将出现高比例的针状铁素体;且在大尺寸奥氏体晶粒中的非金属夹杂物上形核,高密度微小的夹杂物(1μm以下)的弥散分布对其形核更为有利。

RESULTS: The mild traction group showed angulus anterior individual neuron slightly engorged, neuron and nerve fiber morphous was normal essentially; individual substantia alba demyelinates in fragmentis; medullary sheath and auxiliary fibers of nerve root were hydropsia slightly without conspicuous myelinolysis. The moderate traction group demonstrated myelinolysis change in the spinal cord, and neurofibras lined up chaos, auxiliary fibers disaggregated, neuron dropsy, tigroid body disappeared with karyopycnosis and anachromasis in neurons; nerve root showed myelinolysis. The severe traction group displayed a great demyelination region; anterior motor neurons with karyopycnosis and anachromasis; myelinated nerve fiber of nerve root shrinked, decreased in number, with severe demyelination changes.

结果:轻度牵拉组牵拉侧前角个别神经元稍肿胀,神经元及神经纤维形态基本上正常,个别白质小片状脱髓鞘样改变,神经根髓鞘和轴索轻度水肿,脱髓鞘不明显;中度牵拉组白质有脱髓鞘改变,神经纤维排列紊乱,轴索崩解、断裂;灰质神经元水肿明显加重,前角运动神经原细胞尼氏体消失,核固缩、深染,神经根髓鞘和轴索水肿,局部轻度脱髓鞘改变;重度牵拉组牵拉侧白质为不规则的大片脱髓鞘区,前角运动神经元胞体固缩、变形、核深染,神经根有髓神经纤维萎缩、数目减少,重度脱髓鞘改变。

Ultrastructurally, the chalazal border of the mature egg cell demonstrates regularly discontinuous regions in which exist a great number of single or double-membrane vesicles.

卵细胞中的质体多含淀粉粒,精细胞中的质体均不含淀粉粒。DNA荧光显示,卵细胞质中具丰富的类核并可以断定环状类核为线粒体DNA。

The chromosome number is 2n=96, belonging to hexaploid species (2n=6x=96), and the morphological difference is not obvious among the 96 chromosomes. All the chromosomes are metacentric and submetacentric. Relative length ratio of the longest to the shortest chromosomes is 2.10. There is no satellite chromosome in all the 96 chromosomes. The karyotype formula is 2n=96=60m+36sm, the karyotype type is 2B, a higher karyotype evolution.

结果表明,该入侵种的染色体数为2 n=96,属六倍体物种,各染色体间形态差异不明显,均为中着丝粒或近中着丝粒染色体,最长与最短染色体相对长度比为2.10,全套染色体未见随体,核型公式为2 n=96=60 m+36 sm,核型类型为进化程度较高的2B型。

Core particles ) Particles resulting from micrococcal nuclease digestion of nucleosomes, consisting of 140-bp DNA and the histone octamer of a nucleosome.

在真核生物中,双螺旋的 DNA 分子围绕一蛋白质八聚体进行盘绕,从而形成特殊的串珠状结构,称为核小体。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果:细胞接触肽段后存活率下降28.9%;光镜下可见细胞贴壁不良,胞体缩小,细胞突起断裂缩短;荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞;电镜下可见胞质中出现空泡样结构,细胞染色质浓集于核膜内侧并裂解成碎块状;流式细胞仪检测细胞出现亚二倍体峰,DNA电泳出现梯状带。

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