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During capacitation Ca〓 was present on the outer surface of IAM and in the released acrosomal contents.

精子获能前内存Ca〓主要分布于顶体外膜内侧和顶体内膜与核膜之间,并在获能和顶体反应过程被动员利用;顶体反应精子Ca〓分散存在于释放的顶体内容物中和结合于顶体内膜外侧,可能与顶体酶的活性有关;(9)。

In eukaryotes, DNA left-handed chirality by means of histone proteins around octamer to form a stable nucleosome structure.

在真核生物中,DNA按左手手征性的方式,缠绕在组蛋白八聚体的周围,形成稳定的核小体结构。

One of the most important issues concerning investigation of chromatin is how nucleosomes are distributed across genome?

关于核小体的研究中,一个非常重要的问题就是核小体在基因组上的位置是如何分布的?

In mouse, the position of first polar body (PB1) and MⅡ nucleus or spindle is moveable and thus it is difficult to enucleate beneath the PB1. In this study, the 3% sucrose treatment was prepared for enucleation.

在小鼠的卵母细胞中,第一极体(PB1)与MⅡ期核或纺缍体的位置是不固定的,因此导致了去核的困难。

Each of these bundles is called a nucleosome, and many nucleosomes are bound together by the continuing strand of DNA, which forms a string of beads that further coils to form one of two kinds of chromatin, either euchromatin or heterochromatin.

所有细胞核的最显著的特点就是染色体,由DNA和组蛋白所组成的染色质构成,DNA绕行在由4个相似的组蛋白分子结合而形成的柱状的芯上,这一结构称为核小体,核小体之间由连续的DNA链连接,形成串珠样结构,进一步盘曲形成常染色质或异染色质。

In vitro gynogenesis is one of the major methods for haploid production. There isfew report about physiological and biochemical changes of cucumber in vitro gynogenesis.

离体雌核发育是单倍体诱导的主要途径之一,有关离体雌核发育方面的生理生化机制的研究报道尚少。

After prolonged exposure to ANE, the accumulation of hyperploid cells, micronucleated or multinucleated cells were noted concomitantly with centrosome amplification.

另外,长时间的作用下多倍体,多核或微核细胞之有明显的增多,同时也发现常之中心体。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示:VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性;经4mmol/LVPA处理MUTZ-1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞装内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

Based on the model, the main speciality of pearlite transformation of PD3 steel could be obtained.

试验结果表明,PD3钢中的珠光体转变是以多点形核的方式进行的,传统的珠光体单点形核机制不符合PD3钢的实际。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。