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In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中,一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落,异核体中两种核的数量比例应该是1:1的关系;但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明,异核体菌丝两种核的数量比例可能不是1:1的关系。

Generally speaking, the reaction types involvingα-oxoketene--acetals mainly include the regioselective addition-aromatization with organometallic reagents, conjugate addition-cyclization with nitrogen nucleophilicspecies, nucleophilic reactions ofα-carbon, acting as odorless dithiol equivalentsin thioacetalization, Michael additions and [5+1] annulation, as well as the synthetic applications based on the above reactions.

一般来说,基于α-羰基二硫缩烯酮化学的反应主要为:与金属有机试剂的选择性加成反应,与氮亲核体的共轭加成反应,α-碳原子的亲核性及相关反应,烷硫基作为硫醇的替代试剂的应用,α-羰基二硫缩烯酮在碳环、杂环化合物合成中的应用等。

Some of them undergone sustained division at a density as low as 102/ml or even in a single heterokaryon culture.

培养24—48小时后用稀释法挑选出异核体,部份异核体可在低密度(10~2/ml)甚至单细胞条件下持续分裂。

ResultsMorphological features with diagnostic value were established as follows:①Irregular or round-mesh basket type nucleolus;②Malformed nucleus and lobate nucleus with fine bridge;③Perinuclear microfilaments;④Nuclear bodies;⑤Nuclear inclusion bodies;⑥Marked cell pleomorphism arranged irregularly,like a disordered stone pavement;⑦Strange cell and/or giant cancer cell were found;⑧Peculiar papillae or cell clusters were formed;⑨Specific function structure on the surface of cell differentiated poorly.

结果 确定了具有诊断意义的形态学特征:①不规则奇形核仁或网孔状核仁;②畸形核,分叶核伴细桥-核间桥形成;③核旁微丝;④核体;⑤核包涵体;⑥细胞重度多形性,排列紊乱,似乱石铺堆;⑦出现奇形细胞和/或癌巨细胞;⑧形成奇形乳头和奇形细胞簇;⑨细胞表面特异功能结构分化不良。

Results: PC12 cells fragmentation exhibited various evolving phases including totipotential protoplast, endopolyploid,polykaryon, multi-nucleo-endoplast, multinucleocyte, syncytium and multicells. The PCNA-IR was strongly positive in the earlyphase samples of cell fragmentation.

结果:PC12细胞呈现包括全能原生质体、核内多倍体、多核体、核-内质多聚体、合胞体和多细胞裂殖球的一系列演化阶段。

The invention concerns an artificial block comprising a central core (1) having first (11) and second (12) opposite surfaces each provided with an anvil-shaped side leg (21, 22) with a table (21A, 22A), two sloping sides (21E, 21G; 22E, 22G) and two side surfaces (21B, 21C; 22B, 22C), the tables of said side legs being arranged parallel to said first and second opposite surfaces of the core, the inclined sides of said side legs resting on third (13) and fourth (14) exposed opposite surfaces of said core, said core having fifth (15) and sixth (16) surfaces each provided with side legs and front legs shaped like a truncated pyramid with four side surfaces and one front surface (33) parallel to said fifth and sixth surfaces of the core and to the side surfaces of the side legs.

一种保护层块料,包括一个中心核体(1),有相对的第一面(11)及第二面(12),每一面上各有一个砧状侧凸起(21,22),这两个侧凸起各有一个台面(21A;22A)、两个斜面(21E,21G;22E,22G)和两个侧面(21B,21C;22B,22C),所述台面相互平行,而且平行于所述中心核体的相对的第一面及第二面,所述侧凸起的斜面支撑于所述核体的相对的可见的第三(13)和第四面(14)。所述核体还有相对的第五(15)和第六面(16),其上各有一个锥台状的前凸起,该前凸起有四个侧面和一个前表面(33),该前表面平行于所述核体的第五和第六面,还平行于侧凸起的侧面。

Auricularia auricula is of the single-factor controlled mating system according to mating test among the F1 progenies and their parents, combining the method of dyeing the nuclei of the mycelia and observing the nuclear phase under the fluorescence microscope and the method of observing the clamp connection under the light microscope to determine whether the dikaryotic mycelia were formed.

摘 要:从黑木耳种内杂交子H2J3的子实体上单孢分离培养得到F1代52个单核体菌株,将F1子代及亲本(H2,J3)等所有供试单核体菌株的菌丝体两两配对培养,采用核荧光染色观察核相,和光学显微镜观察锁状联合,鉴别菌丝交配反应是否形成双核体菌丝,同时测定了亲本菌株所有F1子代的交配型。

Auricularia auricula is of the single-factor controlled mating system according to mating test among the F1 progenies and their parents, combining the method of dyeing the nuclei of the mycelia and observing the nuclear phase under the fluorescence microscope and the method of observing the clamp connection under the light microscope to determine whether the dikaryotic mycelia were formed.

从黑木耳种内杂交子H2J3的子实体上单孢分离培养得到F1代52个单核体菌株,将F1子代及亲本(H2,J3)等所有供试单核体菌株的菌丝体两两配对培养,采用核荧光染色观察核相,和光学显微镜观察锁状联合,鉴别菌丝交配反应是否形成双核体菌丝,同时测定了亲本菌株所有F1子代的交配型。

Plant protoplasts from several species of plant were fused successfully by electrofusion method, and a good procedure for electrofusion of plant protoplasts in wideinterval chamber was established according to the results.

在植物原生质体的电融合研究中,着重进行了甘蓝与大白菜原生质体的杂交融合和异核体培养,并建立了实用的电融合方法和操作程序

To understand the listed contents:the studies about component of nuclear pore complex; the main functions of gp210;the different configuration models of sequence specific DNA-binding proteins; karyotype and chromosome bandings; configuration of chromatin and dene transcription; nuclear bodies is many subnuclear domains which have different conformation between chromatin.

了解核孔复合体成分的研究;gp210主要的功能;序列特异性DNA结合蛋白的不同结构模式;核型与染色体显带;染色质结构与基因转录;染色质之间的空间发现的许多形态上不同的亚核结构域——核体

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