核仁的

Compared with the corresponding BEAS-2B cells, the shapes of cells and nuclears, the shapes and quantity of cell organs were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The 10th, 15th passage BEAS-2BNNK cells, which had swelled cell and cell organs and abnormal nuclear and enlarged the nucleoli gradually and increased the quantity of the cell organs and activated their function, showed the characteristic of transformation cells. The 20th, 25th passage BEAS- 2BNNKcells, which had obvious aberration of the cell and nucleoli and had cataclasm of nucleoli and decreased cell organs, showed the characteristic of tumor cells.

与BEAS-2B相比较BEAS-2BNNK第1、2、5代细胞及胞核形态、细胞器形态及数量无明显差别；第10、15代细胞出现肿胀，细胞核逐渐变形，出现核畸形，核仁明显增大，边集，细胞器肿胀，数量明显增多，功能活跃具明显的转化细胞特征；第20、25代细胞及胞核明显畸变，出现明显的核碎裂及多个核仁，细胞器明显减少，具明显的肿瘤细胞特征。

Nucleolus disappeared, but some plants in the early diakinesis nucleolus remains high.

核仁消失，但有的植物在终变期的早期核仁仍然很大。

Fig 3 The PG cells untreated with VCR had more nucleoli, nucleolar margination and false intranuclear inclusion.

图3 未经VCR处理的PG细胞，多核仁，核仁边集，核内假性包涵物×10000

The karyotype of the two species of Nelumbo were roughly the same,double chromosome with satellite were observed.

杂种F_1间期核的最多核仁数是其亲本间期核的最多核仁数之和，证明莲属两个种之间不存在NOR竞争或抑制现象。

Nucleophosmin is a protein that shuttles between the nucleus, nucleoplasm and cytoplasm.

核仁磷酸蛋白是一类穿梭于核仁、核质和胞质的蛋白质。

ResultsMorphological features with diagnostic value were established as follows:①Irregular or round-mesh basket type nucleolus;②Malformed nucleus and lobate nucleus with fine bridge;③Perinuclear microfilaments;④Nuclear bodies;⑤Nuclear inclusion bodies;⑥Marked cell pleomorphism arranged irregularly,like a disordered stone pavement;⑦Strange cell and/or giant cancer cell were found;⑧Peculiar papillae or cell clusters were formed;⑨Specific function structure on the surface of cell differentiated poorly.

结果 确定了具有诊断意义的形态学特征：①不规则奇形核仁或网孔状核仁；②畸形核，分叶核伴细桥－核间桥形成；③核旁微丝；④核体；⑤核包涵体；⑥细胞重度多形性，排列紊乱，似乱石铺堆；⑦出现奇形细胞和/或癌巨细胞；⑧形成奇形乳头和奇形细胞簇；⑨细胞表面特异功能结构分化不良。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The incompatible results have been reported even with the same methods But these studies were carried out on the basis that the structure of nucleolus is regarded as a static state. However, the nucleolus is a highly dynamic structure during the cell cycle. It disassembles in the late prophase and reassembles at the end of mitosis.

不过以前对核仁结构和功能的研究大多只是对细胞间期中某个时期细胞的核仁进行静态研究，然而核仁是一个高度动态变化的结构，在细胞分裂前期解体，在细胞分裂末期又开始重建。

The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17 b -estradiol, testosterone and progesterone antibodies.

在卵巢发育早期，卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应；在大生长期和成熟期，卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱，而对雌二醇则显著增强。

Results of study on the behaviour of pine nucleolus during the cell cycle suggested that part of the nucleolar substance, at least, entered the chromosome or attached to the chromosome surface during metaphase and was librated from chromosome to form a new nucleolus during anaphase in higher plants.

对松核仁细胞周期中变化的研究发现，在有丝分裂中期，至少部分核仁成分进入染色体内部或附着在染色体的表面，而在后期从染色体中出来形成新的核仁。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。