核仁

While under natural low temperature (10-20℃), a great deal of abnormalities, including micro nucleoli, scattered and lagged chromosomes and anaphase bridges were detected during PMC meiosis, even though the abnormalities were different between the two cultivars; the ratio of micro nucleoli was 22.88% of average, which was obviously higher than that of control (4.56% of average under normal temperature); and the ratios of univalent and multivalent in diplotene, which increased seriously, were 8.82% and 17.65% of Tainong 1, and 14.52% and 24.19% of Irwin under natural low temperatures.

低温（10~20℃）条件下，减数分裂过程中核仁的行为发生大量异常，微核仁的平均检出率高达22.88%，明显高于常温条件下的平均4.56%；双线期单价体和多价体的发生频率有明显增加，其中台农1号为8.82%和17.65%，Irwin为14.52%和24.19%；减数分裂中期Ⅰ、Ⅱ及后期Ⅰ、Ⅱ均观察到落后染色体，其中台农1号异常率为12.73%、5.63%、15.79%和4.76%，Irwin为11.11%、7.61%、9.52和6.38%。

Fig 3 The PG cells untreated with VCR had more nucleoli, nucleolar margination and false intranuclear inclusion.

图3 未经VCR处理的PG细胞，多核仁，核仁边集，核内假性包涵物×10000

The karyotype of the two species of Nelumbo were roughly the same,double chromosome with satellite were observed.

杂种F_1间期核的最多核仁数是其亲本间期核的最多核仁数之和，证明莲属两个种之间不存在NOR竞争或抑制现象。

The studies on nucleolus focus on the sites of transcription and distribution of rDNA, assembly of ribosomes, and proteomics of nucleolus.

核仁内rDNA的分布、转录定位、rRNA前体的加工及核糖体的装配和核仁的蛋白质组学等都是细胞研究中的重要问题和方面。

Nucleophosmin is a protein that shuttles between the nucleus, nucleoplasm and cytoplasm.

核仁磷酸蛋白是一类穿梭于核仁、核质和胞质的蛋白质。

The materials were first fixed in different fixatives, embedded in paraffin, sectioned serially into 4fi thickness and stained with dyes of safranin and orange G, Heidenhain's iron haematoxylin combined with Mallory's connective tissue stain, buffered Magn-Lillie eosin methyl blue, Feulgen's reaction and methyl green-pyronin.

3凡有核仁外移的细胞，绝大部分为嫌色细胞，而在嗜色细胞中，尤其是在嗜酸性的细胞中极少见到这种现象，因此核仁外移和细胞的种类之间存在着一定的关系。

ResultsMorphological features with diagnostic value were established as follows:①Irregular or round-mesh basket type nucleolus;②Malformed nucleus and lobate nucleus with fine bridge;③Perinuclear microfilaments;④Nuclear bodies;⑤Nuclear inclusion bodies;⑥Marked cell pleomorphism arranged irregularly,like a disordered stone pavement;⑦Strange cell and/or giant cancer cell were found;⑧Peculiar papillae or cell clusters were formed;⑨Specific function structure on the surface of cell differentiated poorly.

结果 确定了具有诊断意义的形态学特征：①不规则奇形核仁或网孔状核仁；②畸形核，分叶核伴细桥－核间桥形成；③核旁微丝；④核体；⑤核包涵体；⑥细胞重度多形性，排列紊乱，似乱石铺堆；⑦出现奇形细胞和/或癌巨细胞；⑧形成奇形乳头和奇形细胞簇；⑨细胞表面特异功能结构分化不良。

hALP is a nucleolar protein, and the nucleolar localization is mediated by its carboxy terminal domain, and hALP could be detected in the nucleoli of many tumor tissues, which is worthy of further investigation.

hALP蛋白具有明显的核仁定位性，并由其特异性的功能域介导，hALP可表达于多种肿瘤细胞的核仁，其意义值得进一步探讨。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17 b -estradiol, testosterone and progesterone antibodies.

在卵巢发育早期，卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应；在大生长期和成熟期，卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱，而对雌二醇则显著增强。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。