核仁

The production technology of biodiesel from litsea cubeba kernel oil with solid acid-esterification and phase transfer catalyst-transesterification was studied.

摘 要：以山苍籽核仁油为原料，采用固体酸催化酯化-相转移催化酯交换反应合成生物柴油。

Objective:To study the significance of Ag-NORS of T lymphocytes in the diagnosis and mornitor of acute lymphocytic leukemia.

目的 ：探讨T淋巴细胞核仁组成区嗜银蛋白在急性淋巴细胞白血病的诊断及病情监测方面的临床意义。

Compared with the corresponding BEAS-2B cells, the shape of cell clone, the way of cell growth, the shape and quantity of cell and nuclear were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The shapes of cells and its nuclears of the 10th, 15th, 20th, 25th passage BEAS-2BNNK cells became round gradually, and the way of cell growth became from radio-form into membraniform and more than one nucleolus appeared.

BEAS-2BNNK细胞在第1、2、5代时细胞克隆形态及生长方式、细胞形态、细胞核形态及数目与相应代BEAS-2B无明显差异；第10、15、20、25代时细胞及胞核逐渐变圆，出现多个核仁，细胞由放射状生长逐渐变为膜状生长。

Volume of cell ill will color is larger, polygonal, afterbirth film is clear, afterbirth qualitative translucence is fine meshy, afterbirth nucleus shrinkage, visible nucleus channel and nuclear different, nucleolus is unidentified show; and acidity of qualitative be addicted to of afterbirth of acerbity cell of be addicted to submits fine grained form, apparent nuclear week sky is dizzy it is thus clear that.

嫌色细胞体积较大，多角形，胞膜清楚，胞质半透明细网状，胞核皱缩，可见核沟及核异型，核仁不明显；而嗜酸细胞胞质嗜酸性呈细颗粒状，可见明显的核周空晕。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型，胞质丰富，胞核成圆形或椭圆型，核仁清楚，甲苯胺蓝呈异染性，阿尔新蓝8Gx 染色阳性，细胞外基质Ⅱ型胶原免疫组化染色阳性，Ⅰ型胶原染色阴性。

Within these aggregates were large mononucleate cells and multinucleate giant cells with one to several nuclei, often with prominent nucleoli, and abundant granular eosinophilic cytoplasm.

肿瘤结节内含有单核细胞和多核巨细胞，常见明显核仁；胞浆丰富，含嗜酸颗粒。

The results showed that pendimethalin is a herbicide interfering cell division,The mito-sis of pendimethalin treated root was arrested at metaphase with contracted and condensed chromo-somes and produced a series of abnormal,polymorphic cells such as multinucleate,multinucleolus,micronucleus and polyploid cells.

结果表明，除草通是一种影响细胞有丝分裂的除草剂，其作用方式在于阻止分生组织细胞从前期向中、后、末期的过渡，引起染色体凝集、短缩以及多核、多核仁等一系列畸形与异常变化。

Senescent cells undergo a number of morphological changes, such as increase in cell size, increase in nuclear and nucleolar size, loss of the ability to utilize all the available growth surface, and appearance of multinucleated cells.

衰老细胞进行了一些形态学改变，如增加细胞大小，增加在核和核仁大小，损失的能力，利用一切可用的增长，从表面上看，和外观的多核细胞。

Key words〕 neoplasma;rDNA transcriptional activity;CD＋4;lymph cell;image analysis

关键词〕癌症；图像分析；Th(CD+4)；淋巴细胞；rDNA转录活性；核仁银染强度

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。