核丝

Henan Xinxiang 453007 Abstract The karyotype and the number of Humulus scandens

采用传统的核型分析技术，分析了葎草茎尖有丝分裂中期染色体的核型。

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

This means that the ACA antiserum can actually and specifically recognize the protein components of centromere/kinetochore.

这看来是着丝粒蛋白在单细胞真核生物间期核中的分布上的特点。小眼虫细胞质中有一些阳性反应的颗粒，可能为线粒体或叶绿体的成份。

Methods: The total polysaccharide was extracted with distilled water from mycelia of paecilomyces cicadae. The colorimetric MTT was used to analyze the proliferative activity of PBMC, U937 and K562 which were treated with PCPS of 7 concentrations respectively in vitro. ELISA was used to detect the content of hTNF-α, hIFN-γ from supernatant of culturing PBMC that was treated with PCPS for 44 h.

用水提取蝉拟青霉菌丝体总多糖，以MTT法测定不同浓度总多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的影响，ELISA法测定多糖作用44h后，外周血单个核细胞培养上清液中hTNF-α、hIFN-γ的含量。

Endomitosis The duplication of chromosomes without division of the nucleus, causing polyploidy and leading to an increase in nuclear and cytoplasmic volume.

核内有丝分裂：在细胞核不分裂的情况下进行染色体复制，产生多倍体并使细胞体积增大的一种有丝分裂形式。

The erasion of the mark likely was crucial in the process of the genome epigenetic reprogramming.

总之，TBP在有丝分裂中期染色体上的残留可能是分化细胞特有的细胞记忆标记；这一标记在核质重组胚的核重序过程中被去除。

Effect of Different Sowing Date and Densities to the Yield of Avena nuda L .Variety Huazao No.2;2. The paper analyses the chromosome karyotype of Avena nuda L .

对莜麦染色体的核型进行了分析，结果表明其染色体数为42，为六倍体，包括11对中部着丝粒染色体，9对近中部着丝粒染色体和1对近端着丝粒染色体，其中有3对染色体带有随体。

Yantai Normal College,264025,ChinaABSTRACT Most epithelial nuclei of Nuttallia olivacea are elliptic in shape and the chromatin is filiform.Most nuclei of connective tissue are round and have Feulgen positive granules.

摘 要 紫彩血蛤的鳃上皮细胞核多为椭圆形，核内染色质呈丝状；结缔组织细胞的核多为圆形，部分核内有块状Feulgen阳性颗粒。

X chromosome was a large submetacentric chromosome. Y chromosome was a small metacentric chromosome.The relative length of chromosome was measured by AutoCAD software. The relative length of autosomes ranged from 1.545% to 4.921% in Yanbian yellow cattle and from 1.637% to 5.855% in Liyan hybrid cattle.

结果表明，延边黄牛和利延杂交牛二倍体染色体数目2n=60，公牛核型为2n=60，XY，母牛为2n=60，XX，其中29对常染色体均为端着丝粒染色体，X染色体为较大的亚中着丝粒染色体，Y染色体为较小的中着丝粒染色体。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷