样品鉴定

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Methods: The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitosis active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity.

采用无限稀释和平板划线法分离微生物样品，利用稻瘟霉模型进行活性筛选；并对其中的活性菌株32-11-2-2的形态、培养特征以及生理生化特征进行研究，使用16S rDNA序列分析的方法对菌株32-11-2-2进行了分类鉴定。

More information about their structure and function relationship will be meaningful in understanding the molecular mechanism of ion pump and conformation alteration during ion transport and sensory transduction. In this paper, we report screening strains from samples collected from Ebi lake and Altun moutain Aqik lake, Xinjiang Province, and got 60 clonies from them. We choose 8 of them for further investigation and confirmed that they all belong to the Halobacteriaceae genus and through comparasion of several minute diffrerence medium we had the optimal medium for their growth.. Through this procedure we had established a set of methods for screening clonies from wild environment.and isolation and purification of these archaerhodopsin. Furthermore we used PCR method to identify their DNA sequence.

我们从新疆艾比湖和海拔4250米的阿尔金山阿其克库勒湖中采集到水样、土样和冰样，从中分离出60个单菌落，挑选了8个单菌落进行活化和扩大培养，确证了它们属于嗜盐菌属；确定了它们的最适生长条件；建立了一套完整的从野生环境样品中筛选嗜盐菌菌株的完整方法；摸索出了提取它们中的古紫质蛋白的方法；进行了初步PCR鉴定，为后续研究奠定了良好基础。

The experiment indicated,Using the anthrone color method observable knowledge sample sugar content is 8.8%,According to paper chromatography, Appraises its principal constituent using the anthracene for the glucose, the fructose, the mannose

实验表明，利用蒽酮比色法可测知样品的含糖量为8.8%，根据纸色谱法，鉴定出其主要成分为葡萄糖、果糖、甘露糖。

Five species belonged to five genera were extracted from soil samples which were Helicotylenchus digonicus, Tylenchorhynchus maximus, Psilenchus hilarulus, Filenchus vulgaris and Aphelenchus avenae. Except A.

结果表明：从洋葱根际土壤样品中共分离鉴定出线虫5个属5个种，包括喜悦平滑垫刃线虫、普通丝尾垫刃线虫、双角螺旋线虫、最大矮化线虫和燕麦真滑刃线虫。

With the observation of the outward appearances, it can be known roughly that if the sample was added with adulterations. Since the beeswax dissolves in the benzene but the adulterations, such as gypsum, starch and so on do not, these can be determined. By the verification of acid value and alcohol dissolve ability, the acid or non acid added can be determined. By the change of saponification value, the ester or non ester added can be determined. By the carbonification degree in the concentrated sulphuric acid, the mineral wax, such as paraffin wax added can be determined.

通过样品外观的观察初步定性是否掺有杂质；利用纯蜂蜡在苯中溶解，而掺入的石膏、面粉和淀粉等杂质在苯中不溶而定量鉴定这类杂质；由酸值变化和乙醇溶解物的量，测定掺入的硬脂酸等酸性物质或其它的非酸性物质；由皂化值变化，定量测定掺入的动物脂等酯类物质或其它的非酯类物质；根据蜂蜡中的烃类在浓硫酸中可被炭化，加入的石蜡等矿物蜡不被炭化来测定此类掺杂物。

The percentages of insect fragment occurrence of various body parts were: antenna, 34.5%; mandible, 8.8%; legs, 43.4%; wings, 5.3%; cerci, 1.8%; and ovipositors, 0.9%.

昆虫碎片经鉴定分析后，各目昆虫碎片样品前三目之百分比分别为，直翅目47.8%、鞘翅目21.7%、革翅目15.2%。

Thirteen species of Chaetognatha were identified, and the species composition in the collected specimens was simple.

结果表明，该海域毛颚类种类组成较为简单，样品中共鉴定出13种，其组成无明显季节变化。

Through study on ecological diversity of myxobacteria in some areas of Hebei, Yunnan, and the Qinghai Tibet Plateau, more than 150 strains of 10 genera ( Archangium, Myxococcus, Cystobacter, Corallococcus, Melittangium, Sorangium, Polyangium, Chondromyces, Angiococcus and Stigmatella ) of Myxococcales were isolated from 42 samples, including some special strains that have never been described before.

通过对河北、云南、青藏高原不同地区的粘细菌进行生态多样性研究，从 42个样品中分离得到了 10个属(Archangium、Myxococcus、Cystobacter、Corallococcus、Melittangium、Sorangium、Polyangium、Chondromyces、An giococcus、Stigmatella)的 15 0余株粘细菌，其中包括一些尚未有描述的菌株，有待鉴定。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征；利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类；对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增，克隆到pGEM-T载体并进行测序；应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。