株

The mutants were further screened through repeated fermentations as well as the measurement of their physical properties. The results showed that some major fermentation indexes of strain HJ1615-d4 such as alcoholicity, fermenting power,total acids and flavoring components etc. were almost the same as those of the original strain. However, its urea yield was only 15.41 mg/L, louer 50 % than that of the original strain.

对这4株突变株进行发酵栓实验，并测定其发酵综合性能，其中菌株HJ1615-d4在发酵力、总酸、酒精度以及风味物质等综合指标方面保持了亲株的优良性状，同时产尿素的量为15.41 mg/L，低于出发菌株产尿素量50 %以上。

In a commercial nursery using the peat moss-plug system, these three strains also showed their ability to promote seedling growth following either seed bacterization or peat moss drenching, and their populations in peat moss maintained at high levels (except strain RS65 for 30 min seed soaking ) within 21 days after seeding. The above 14 strains that were capable of promoting tomato growth were tested for their ability to control bacterial wilt of tomato.

自台湾中部不同地区数种作物分离得到之396株根栖细菌，被覆於番茄种子后，利用套袋塑胶培养皿之系统及含泥炭土栽培介质之穴盘系统於生长箱筛选，共有 14株菌株在重复筛选过程中均能提高种子发芽率及促进根生长之效果，其中RS4、RS65及RS70三菌株表现最佳，可增加根长度、茎长度、植株鲜重及乾重(RS65菌株除外)。

Among these,there were 432 strains of Gram positive coccus,accounting for 21.7%;445 strains of Enterobacteriaceae,accounting for 22.4%;621 strains of Non-ferment bacillus,for 31.2%;488 fungus,making up 24.5%.

结果：2002年共分离获得1989株病原菌，其中革兰阳性球菌432株，占21.7%；肠杆菌科细菌445株，占22.4%；非发酵糖革兰阴性菌621株，占31.2%；真菌488株，占24.5%。

The columna soil study showed that strain NBT had the ablity of potassiumuptake ,making losed potassium from the soil inoculated with the bacterium wasdecreased by

通过土柱试验发现，硅酸盐细菌NBT菌株还具有保钾功能，供试土壤接菌处理后的土壤滤液中流失的钾比对照减少35.5-56.5％，经方差分析，差异达显著水平；另外，硅酸盐细菌NBT菌株与农药的相容性试验表明，无论在大田农药施用浓度和残留浓度内的丁草胺、绿黄隆、三唑酮粉、多菌灵、马拉硫磷等农药对NBT菌株没有抑制作用；乐果、甲基1605在大田施用浓度下，对NBT菌株有一定抑制作用，而在残留浓度下，对NBT菌株无明显抑制作用。

It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示，菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三种葡聚糖蔗糖酶，其中前两者同时存在于上清液中和细胞表面，而后者只存在于上清液当中；菌株DM1-2可以产生两种葡聚糖蔗糖酶，分子量分别为183kDa和142kDa，前者同时存在于上清液中和细胞表面，而后者仅能存在于细胞表面；菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三种葡聚糖蔗糖酶，前两者属于胞外酶，而后者与细胞相连；与菌株6055相似，菌株L4可以产生两种同时存在于上清液和细胞表面的葡聚糖蔗糖酶，分子量分别为145 kDa和136kDa，而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

According to their individual shapes, they are Gram positive, catenulate rod-shaped bacteria that can produce elliptical endospore in the middle position. These characteristics are similar to Bacillus cereus' described in Bergey's Manual of Systematic Bacteriology. And the colony States, physiological and biochemical characteristics of the two strains also accord with Bacillus cereus', which suggests that they might be identified as Bacillus cereus.

通过菌株的个体形态观察，发现这两株菌是可以在细胞中间产生不膨胀的椭圆形芽孢的革兰氏阳性杆菌，且相互成链，这与伯杰氏手册中对蜡状芽孢杆菌的描述相似，另外对这两株菌的培养特征、生理生化特征的研究，也表明这两株菌符合蜡状芽孢杆菌的特性，故初步鉴定这两株菌为蜡状芽孢杆菌。

Interestedly, the clinical diagnosis of this isolate was from a patient with menigoencephalitis. The new strains of the influenza B virus which were isolated in VGH-Taipei, during 1999 winter to 2001 spring were then used for further analysis.

此株病毒株是由一位患有脑膜脑炎的病童所分离到的，这株B/Taiwan/3143/97在HA基因的演化分析中远独立於当时其他的病毒株（B/Taiwan/21706/97）；且其在核酸及胺基酸序列上与其他的分离株比较也有很大的不同。

Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.

用三种不同毒力的钩体（致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株）分别感染体外培养的豚鼠腹腔巨噬细胞，并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min，通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数，检测钩体对巨噬细胞吞噬功能的影响；感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化；用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。

The two can cause ultrastructural pathogenesis widely in many organs and tissues such as hepar, pancreas, lien, ren, pulmo, cerebral, cerebellum, cor, muscular and so on, of them ,caused by i~olates YG97 and Y98 more obvious in epithelial cells of digestive tract and unobvious in epithelial cells of respiratory tract ,but ultrastructural pathogenesis caused by NDV strain F48E8 ,more obvious and more serious in glandularis and muscularis ventriaculi and in epithelial cells of respiratory tract .

将鹅源分离株YG_(97)、鸡源分离株Y_(98)和NDV强毒株F_(48)E_8感染SPF鸡，运用扫描电镜和透射电镜观察该二株病毒对机体细胞的影响，结果显示三株禽副粘病毒均引起机体肝、胰、脾、肾、肺、小脑、大脑、心和肌肉等实质器官组织细胞的超微病变，其中YG_(97)和Y_(98)引起消化道粘膜上皮细胞发生微绒毛脱落、纤毛脱落、分泌颗粒增多、细胞游离面破溃等显著的超微病变，引起气管粘膜上皮细胞的不显著超微病变；F_(48)E_8强毒株引起胃粘膜显著而严重的超微病变；还引起气管粘膜上皮严重的超微病变。

The VVA0326- mutant carrying a deletion of the GGDEF domain displayed smaller colony morphology on LB plate while compared to the wild type. No difference in colony forming units was found indicating that growth rate is not likely a major factor to confer the phenotype of small colony. In addition, VVA0326- appeared to swim better than wild type bacteria on the swimming plate. VVA0326- grew better, while VVA0328- mutant, which carried a deletion of the EAL domain, grew worse than wild type when the bacteria were cultured on CAS plate at 30°C. This implied that changes in surface structures of the mutants affected the bacterial susceptibilities to HDTMA contained in CAS plate.

缺损了包含一个GGDEF功能区域的突变株 VVA0326-，培养在LB的洋菜胶上，呈现出较小的生长菌落，在测定单位菌落数的结果发现，VVA0326-在生长速度上，相较於野生株并没有不同，因此我们推论生长速率并不是造成菌落变小的主要因子，除此之外，VVA0326-在游泳的分析培养皿中游的比野生株快，在CAS的分析培养皿上，与野生株比较，VVA0326-有比较好的生长状态，而缺损了包含一个EAL功能区域的突变株VVA0328-，却有被抑制生长的现象，可能是因为突变株影响了表面的结构，进而改变了细菌对於HDTMA的敏感层度。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。