株

Although env gene correlates with cell tropism, co-receptor usage and HIV syncytium inducing, it may have relationship with the whole genome, whether these phenotypes are present or not.

虽然HIV外膜基因与毒株的细胞嗜性、受体使用，与介导融合等特性有关，但这些表型的体现与否，还与毒株的整个基因组背景有关。

When EMS was applied, it was found that 2.0% EMS resulted the highest microkernel rate (15.6‰). Treating the materials with 2.0% EMS for 4 hours could lead to semi-lethal. Cytological observations found that chromosome aberration phenomena such as single microkernel, double microkernel, three microkernel, chromosome bridge, resort chromosome and syncytium were presented in the mutants.

EMS诱变途径中，浓度为2.0 %处理时间为4 h的组合获得最高的微核率（15.6‰），EMS浓度为2.0 %处理时间为4 h的组合使材料达到半致死；细胞学的观察看到了单微核、双微核、三微核、染色体桥、滞留染色体和合胞体等染色体畸变现象，再生苗中发现了1棵变异株。60Co辐射的诱变途径中，20Gy的剂量使不定芽半致死，再生苗中发现了1棵变异株。

PCR products of isolates that rpoB gene mutations were not found with PCR-SSCP were sequenced using double deoxidization chain terminating.

并对其中19株SSCP阴性的耐RFP株用双脱氧末端终止法进行测序分析。

PCR products of isolates that rpoB gene mutations were not found with PCR-SSCP were sequenced usˉing double deoxidization chain terminating.

并对其中19株SSCP阴性的耐RFP株用双脱氧末端终止法进行测序分析。

The analysis methods for the determination of micro or trace elements in high moisture jellyfish were developed. The fatty acid compositions in difderent parts of fresh jellyfish were determined by GC/MS method. Thirty-five fatty acids were identified, and most of them were found in R. esculentum jellyfish for the first time. Especially, two unusual very long chain polyunsaturated fatty acids that were never detected in the other jellyfish also were determined. Amino acids were abundant in R. esculentum jellyfish, especially containing sulfur amino acids, and could be supplied for human diet. The polysaccharide in umbrella part of jellyfish was composed of glucose, galactose and uronic acid, and its molecular weight was 40,000, but the polysaccharide of the oral arms part consisted of glucose, mannose and glycuronic acid, and its molecular weight was 43,000. Above-mentioned data were never reported. The ethanolic extract of oral arms part of jellyfish were extracted by different polar solvents (petroleum ether, acetic ether, n-butanol), and antibacterial activity was tested to these extracts by four species of terricolous pathogenic bacilli and three species of botanic pathogenic fungi. The result demonstrated that the petroleum ether extract had certain bactericidal activity for two species of pathogenic bacilli, and n-butanol extract had certain inhibited activity on apple rot pathogenic fungus.

建立了 高含水量的海蜇产品中微量、痕量元素成分测定的分析方法；采用 GC/MS 方法测定了新鲜海蜇不同部位的脂肪酸组成，共鉴定出 35 种脂肪酸，其中大多数脂肪酸是首次在海蜇中被检测到，尤其是两种不常见的 C24：5 超长链多不饱和脂肪酸的分析和鉴定在其它水母种属中也从未见报道；海蜇三个部位中氨基酸成分齐全，含量丰富，含硫氨基酸含量较高，可与其它食物蛋白质的氨基酸互补；其中海蜇皮多糖是由葡萄糖、半乳糖和糖醛酸组成，分子量为 40，000，海蜇头多糖是由葡萄糖、甘露糖和糖醛酸组成，分子量为 43，000，以上工作均未见报道；利用石油醚、乙酸乙酯、正丁醇三种不同极性溶剂分别萃取海蜇头乙醇浸提物，用纸碟法和生长速率法分别对四株陆源病原菌和三株植物病原真菌进行了抑菌实验，结果表明海蜇头石油醚提取物和正丁醇提取物具有一定的抑菌活性。

The analysis methods for the determination of microor trace elements in high moisture jellyfish were developed. The fatty acid compositions indifderent parts of fresh jellyfish were determined by GC/MS method. Thirty-five fatty acids wereidentified, and most of them were found in R. esculentum jellyfish for the first time. Especially,two unusual very long chain polyunsaturated fatty acids that were never detected in the otherjellyfish also were determined. Amino acids were abundant in R. esculentum jellyfish, especiallycontaining sulfur amino acids, and could be supplied for human diet. The polysaccharide inumbrella part of jellyfish was composed of glucose, galactose and uronic acid, and its molecularweight was 40,000, but the polysaccharide of the oral arms part consisted of glucose, mannose andglycuronic acid, and its molecular weight was 43,000. Above-mentioned data were never reported.The ethanolic extract of oral arms part of jellyfish were extracted by different polar solvents(petroleum ether, acetic ether, n-butanol), and antibacterial activity was tested to these extracts byfour species of terricolous pathogenic bacilli and three species of botanic pathogenic fungi. Theresult demonstrated that the petroleum ether extract had certain bactericidal activity for twospecies of pathogenic bacilli, and n-butanol extract had certain inhibited activity on apple rotpathogenic fungus.

建立了高含水量的海蜇产品中微量、痕量元素成分测定的分析方法；采用 GC/MS 方法测定了新鲜海蜇不同部位的脂肪酸组成，共鉴定出 35 种脂肪酸，其中大多数脂肪酸是首次在海蜇中被检测到，尤其是两种不常见的 C24：5 超长链多不饱和脂肪酸的分析和鉴定在其它水母种属中也从未见报道；海蜇三个部位中氨基酸成分齐全，含量丰富，含硫氨基酸含量较高，可与其它食物蛋白质的氨基酸互补；其中海蜇皮多糖是由葡萄糖、半乳糖和糖醛酸组成，分子量为 40，000，海蜇头多糖是由葡萄糖、甘露糖和糖醛酸组成，分子量为 43，000，以上工作均未见报道；利用石油醚、乙酸乙酯、正丁醇三种不同极性溶剂分别萃取海蜇头乙醇浸提物，用纸碟法和生长速率法分别对四株陆源病原菌和三株植物病原真菌进行了抑菌实验，结果表明海蜇头石油醚提取物和正丁醇提取物具有一定的抑菌活性。

Pf7-14 was chosen to study the spatial-temporal colonization patterns on both rice flag leaves and rice stems under greenhouse conditions by spraying 1×10〓 cfu/ml bacterial suspension of Pf7-14 at late booting stage, periodical sampling, and recovering the bacteria through diluting-plate method as well as graphic test and the Shapiro-Wilk test for normality of the data.

以菌株Pf7-14为代表，在温室条件下，在水稻孕穗后期喷雾浓度为1×10〓cfu/ml的菌悬浮液，然后定期取样，用稀释平板法在含抗生素Nal的培养基上回收细菌，并通过图形测试和Shapiro-Wijk正态测试，测定了该菌株在水稻剑叶和茎基部的时间、空间定殖型。

And two plants among fourty-six were the tetraploid.

在获得的46株再生苗中，有2株为四倍体植株。

The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Zhungeer had the lowest resistance and highest damage (88.03%). Relation analysis showed that the damage index was not significantly related to plant height or thrips density.

对不同品种苜蓿的受害指数与株高和虫口数量的相关分析表明，受害程度与株高及虫量无关。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。