株

Methods The selected microorganisms were cultivated at 37℃ in culture media that was put different concentration of sodium arsenite,and detected arsenic accumulation in cells by ASA.

各菌株接种在不同浓度含砷培养基中，观察其对砷的耐受性及砷对各菌株的生长影响；利用超声波细胞粉碎机震碎菌体后，用超低温捕获原子吸收分光光度仪检测砷含量，用荧光光谱检测器检测砷形态。

S extract had an inhibition effect against Sphaerotheca fuligenea,The inhibition rate was 100% when extract concentration was 20mg/mL and the inhibition rate got to 67.66% even at the concentration of 2.5mg/mL. The extract of E. humifusa had different preventive effects against four strains of S. fuliginea from four districts in Shanghai. The preventive effects on the Qibao and Baoshan were better than those of Nanhui and Qingpu. The microorganism inhibition experiment by filter papers indicated that the extract of E. humifusa had higher inhibition effect against bacteria than on pathogenic fungus. Diameters of the zone of inhibition of salmonellas, Echerichia coli and Staphylococcus aureus were 15.77mm, 12.27mm and 10.53mm respectively. The extract of E. humifusa had a certain effect against Colletotrichum lagenarium, Botrytis cinerea, Rhizoctomia solan and Pyricularia oryza but no effect against Helminthosporium maydis and Gibberella zea. The 4 cation exchange resins and ethanol deposition were used to isolate the active fractions from supernate after alkali deposition.

提取液对黄瓜白粉病菌体外分生孢子萌发抑制实验表明地锦提取液对黄瓜白粉病菌分生孢子的萌发有很好的抑制作用，抑制率随着地锦提取液浓度的增大而增高，当浓度为20mg/mL时，抑制率达到100%，即使在浓度为2.5mg/mL时，抑制率也达到了67.66%；室内盆栽苗期预防性实验表明了地锦提取液对上海4个不同地区的黄瓜白粉病菌均具有不同程度的抑制效果，对七宝和宝山地区的菌株抑制效果优于南汇和青浦地区的菌株；用滤纸片法测定了地锦提取液的抑菌谱，实验结果表明地锦提取液对供试的4种植物病原真菌：黄瓜炭疽病菌、黄瓜灰霉病菌、水稻纹枯病菌和水稻稻瘟病菌均具有一定的抑制效果，而对玉米小斑病菌和小麦赤霉病菌没有抑制效果，对供试的3种人类致病细菌沙门氏菌、大肠杆菌和金黄色葡萄球菌均具有很好抑菌效果，抑菌圈直径分别为：15.77mm， 12.27mm和10.53mm。

A total of 29 isolates were selectively obtained from these rhizosphere soli samples based on their growth on nitrogen-free medium and their resistance to 100℃ for 10-15 min.

首先利用芽孢杆菌中芽孢的抗热性将土壤溶液在100℃沸水中煮10-15分钟，然后用选择性无氮培养基进行初筛得到29株菌落形态不同的菌株

This selection strongly inhibits the growth of arg-13 under stringent sorbose/glucose condition but allows arg-13 to grow under spot test conditions.

此法在严紧山梨糖/葡萄糖条件下能强烈抑制arg-13突变株生长，但在斑点试验条件下允许arg-13突变株生长。

Basing on twice surveys of HIV-1 molecular epidemiology, HIV CRF01_AE strain has been introduced into inland neighboring provinces from southeastern coast areas and southwester border area, the prevalence of HIV CRF01_AE strains are increasing.

据全国两次分子流行病学调查结果表明HIV CRF01_AE重组型株正在从南部及沿海省市向周围省市扩散，CRF01_AE重组型在全国所占比例也有所增加，在福建省HIV CRF01_AE重组型为主要的流行株。

As a result depart leads the 0.32% rises to 2006 0.81%; from 2000 to guard ill area in order to weigh disease detached bacterium is most, 68.9% what take individual plant of total detached bacterium, it is ill area of kidney internal medicine next, occupy 13.5%; to be in example sending check 62.3% what with uric fluid specimen individual plant counts detached bacterium to hold total detached number at most, it is phlegmy fluid next 10.2%.

结果分离率从2000年的0.32%上升到2006年的0.81%；以重症监护病区分离菌株最多，占总分离菌株的68.9%，其次为肾内科病区，占13.5%；在送检样本中以尿液标本分离菌株数最多占总分离数的62.3%，其次为痰液10.2%。

Eighteen isolates of Phytophthora were isolated from diseased sponge gourd in Shenyang. Two typical Phytophthora strains that caused sponge gourd wilt were chosen in this experiment.

从沈阳地区丝瓜疫病的病组织及土壤中分离18株疫霉菌，从中选取2个代表菌株进行分类鉴定。

Results Four hybridoma cell lines stably secreting McAbs against sporozoites were obtained: Easp-3G3 and Easp-5G10 belonging to IgG1, Easp-3H6 belonging to IgG2b, Easp-5H4 belonging to IgG2a. All four McAbs bound with E. acervulina sporozoite protein, but the Easp-5H4 McAb showed cross reactivity with E.

结果 获得4株可稳定分泌单克隆抗体的杂交瘤细胞株，其中Easp-3G3、Easp-5G10为IgG1 类，Easp-3H6为IgG2b 类，Easp-5H4为IgG2a 类。4株单克隆抗体均能与堆型艾美球虫子孢子的抗原蛋白反应，但仅Easp-5H4能与柔嫩艾美球虫子孢子抗原蛋白反应。

This muant strain also lost the ability of sporulation and production of crystal. At the same time, the production of ICPs is severely decreased by SDS-PAGE analysis.

该突变株也丧失了产生芽胞和晶体的能力，SDS-PAGE分析表明突变株G03的杀虫蛋白产量也非常低。

These results suggest T. asperellum TA strain have an excellent potential to be used as biocontrol agents for the control of stem rot disease of Taiwan Anoectochilus.

本研究所提供之试验结果显示，研究中所用之Tasperellum TA菌株确为金线连茎腐病生物防治上极其应用潜力之菌株。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。