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Inan attempt to select more efficient biocatalysts ,the hydantoinase and carbamylase genes fromBurkholderia pickettii were cloned in Escherichia coli, achieve a co-experssed strains. The geneswere assembled to give the carbamylase gene preceding the hydantoinase gene. The recombinantstrains stably and constitutively produced the two enzymes and efficiently converted thecorresponding hydantoins onto p-hydroxyphenylglycine and phenylglycine .

我们试着寻找更快更有效的转化菌,把来源于皮氏伯克霍尔德氏菌的表达该两种酶的两个基因同时克隆到大肠杆菌中,获得共表达两种酶的基因工程菌,两个基因的顺序是 Dcase 基因在 Dhase 基因的前面,同一个启动子。

To scientifically control and prevent Newcastle disease,this study researched the molecular evolution characteristics of Phosphoprotein gene of Newcastle disease viruses.

对1997~2007年国内分离的13个NDV毒的P基因进行扩增测序,与15个已发表的国内外不同时期的NDV毒P基因进行核苷酸和推导的氨基酸遗传变异分析及分子特性研究。

The major gene of CTXΦ receptor Tcp (toxin-coregulated pilus), tcpA, was detected by polymerase chain reaction analysis.

然而在本实验室研究中,于不含ctxAB基因的El Tor型霍乱弧菌中发现部分菌含有RS基因同源序列,为此对这些菌作CTXΦ基因组的进一步研究。

Furthermore, pistillate trees entered the dormant phase earlier than staminate ones.

而且,雌进入各个休眠阶段的时间都比雄早。

To make comparison of adaptability of the plastid-less mutant and the wild type strain, we studied their growth characters under different culture conditions, found that the difference of their growth in a medium rich in organic substances are hardly detectable and that the loss of plastid exerts only a weak effect, if any, on the heterotrophic growth.

为比较完全失去质体结构的突变和野生型的适应力,我们对二者在不同条件下的生长特性进行了研究,结果发现野生型与突变的生长在有机质丰富的培养液中差别微弱,质体的丢失并不产生显著影响。

Losing competence might affect their β-lactam resistance, and the consequences were different in different strains.4. To further understanding when and where pneumococci formed transformation in vivo so as to study the influence of pneumococcal transformation to its virulence, we constructed a pneumococcus 22G itstransformation forming could be reported by EGFP.

肺炎链球菌的感受态形成可诱导ciaH和cpoA的表达增加,其感受态缺陷影响到其对β-内酰胺类抗生素的敏感性,但不同菌影响的结果不同。4、为进一步了解肺炎链球菌在宿主体内转化发生的情况,便于深入研究体内细菌转化对其毒力形成的影响,本研究构建了绿色荧光报告细菌转化发生的肺炎链球菌菌22G。

Isolates from different geographic areas and even some isolates in the same area showed considerable amount of DNA polymorph ism.

不同地区的分离甚至同一地区的某些分离存在相当大的DNA多态性。

The research was performed to select certain typical bacterial strain as the vaccine strain to produce polyvalent inactivity vaccine for the local prevention and counterchecking of the disease on the basis of the separation and identification of streptococcosis suis, in the southern part of Xinyang region of Henan Province.

本研究是在对信阳地区猪链球菌病病原分离、鉴定的基础上,挑选具有一定代表性的菌作为疫苗,研制多价灭活疫苗用于本地区该病的免疫防制。

A primrose by a river's brim, a yellow primrose was to him, and it was nothing more.

河床边那报春花,对他而言就是一淡黄的报春花,不可能是其他东西。

Using TaKaRa LA PCR? in vitro Clonging Kit, a 2.3kb DNA fragment from the mutant was amplified and identified. Sequence analysis indicated that the fragment was composed of a complete proE gene and a partial proA gene.

根据枯草芽孢杆菌93151野生菌proB基因的序列设计并合成引物,利用TaKaRa LA PCR~ in vitro Clonging Kit试剂盒,克隆突变菌的proBA基因,得到一个约2.3kb的DNA片段,序列分析表明该片段包含一个完整的proB基因和不完整的proA基因。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。