株

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Another two field container experiments were conducted to study intraclonal spatial division of labour in Fragaria vesca and Lysimachia christinae with different branching types and Lysimachia christinae at different altitudes in response to reciprocal patchiness of light and nutrients.

结果显示：克隆整合显著增强蛇莓和野草莓胁迫分株的生长，耗-益分析表明对未受胁迫分株没有显著损耗，故整个克隆片段的生长得到显著提高，克隆整合对于克隆植物在高山环境中的种群扩展、生境开拓可能是非常重要的。

The cowpea number per pod, weight per pod, length per pod, number of inflorescence; and the height of main stem, number of branches and node of main stem at seedling-pulling at each single plant were investigated for the yield-calculating, and meanwhile, the analysis of relevant factor and path analysis of the component of yield/plant was conducted.

方法］定株调查每株美国无架豇豆的豆英数、英重、英长、花序数，拉秧时测定主茎高、分枝数及主茎节数，并计算单株产量，进行单株产量构成因素的相关分析和通径分析。

Finally,the WISCONSIN Sequence Analysis Package (GCG,10.2 version),Phylogenetic Analysis Using Parsimony (PAUP,4.0 version) and Treeview (1.5 version) software were used for data analysis,sequence translation,phylogenetic tree drawing and molecular evolution analysis.

本研究结果证明，H9N2亚型人体分离株属于G1-like亚分支的G1-HK小分支，该小分支毒株之间具有很高的同源性，因此该小分支AIV可能具有感染人的可能性，是具有引起人流感暴发潜能的病毒。

By using cluster analysis, the tested cultivars are classified into two groups (bush vineⅠand semi-bush vine Ⅱ) and four sub-groups (Ⅰ1,Ⅰ2,Ⅱ1 and Ⅱ2). Plant type has a more important effect on C. pepo yield formation.

结果表明：反映西葫芦株型的主要指标是株高、茎节长、平均叶面积、功能叶片数和叶片倾角；通过聚类分析把参试品种分为两类4个亚类，即矮蔓Ⅰ（Ⅰ1和Ⅰ2）和半矮蔓Ⅱ（Ⅱ1和Ⅱ2）。

The nitrate non-utilizing mutantas an another added genetic marker was used to select the out-crossed perithecium from self-crossed perithecia. Seven crosses were tested in all, including cross between S×S, MR ×S, MR×MR,HR ×S, as well as HR ×MR. The results showed that no recombinant phenotype was found among all progeny of seven crosses. Progeny segregation of crosses between the parents with different sensitive levels (i.e. MR×S, HR×S,HR×MR) clearly fit a 1:1 ratio of two parental phenotypes, and no segregation was found in the crosses of S×S and MR × MR.

从25个敏感菌株和31个抗性菌株中随机挑选了2个S、3个MR和1个HR，并以硝酸盐营养缺陷型突变体作为另一个遗传标记，按照S×S、MR×MR、MR×S、HR×S、HR×MR等共设计了7个杂交组合，对各杂交后代对多菌灵的敏感性测试发现，在所有杂交后代中均未出现除双亲表现型以外的重组型个体，MR×S、HR×S及HR×MR的杂交后代出现了1∶1的分离比例。

Nature sent him into the world strong and lusty, in a thriving condition, wearing his own hair on his head, the proper branches of this reasoning vegetable, till the axe of intemperance has lopped off his green boughs, and left him a withered trunk, he then flies m art, and puts on a periwig, valuing himself upon an unnatural bundle of baits, all covered with powder, that never grew on his head, but now should this our broomstick pretend to enter the scene, proud of those birchen spoils it never bore, and all coveted with dust,through the sweepings of the finest lady's chamber, we should be apt to ridicule and despise its vanity Partial judges that we are of our own excellencies, and other men's defaults!

当大自然送他入世之初，他是强壮有力的，处于兴旺时期，满头的天生好发；如果比作一株有理性的植物，那就是枝叶齐全。但不久酗酒贪色就像一把斧子砍掉了他的青枝绿叶，只留给他一根枯株。他赶紧求助于人工，戴上了头套，以一束扑满香粉但非他头上所长的假发为荣。要是我们这把扫帚也这样登场，由于把一些别的树条收集到身上而得意洋洋，其实这些条上尽是尘土，即使是最高贵的夫人房里的尘土，我们一定会笑它如何虚荣吧！我们就是这样偏心的审判官，偏于自己的优点，别人的毛病！1.pretend："自认为有能力"的意思，根据上下文的情况可以翻译为"想"

Nature sent him into the world strong and lusty, in a thriving condition, wearing his own hair on his head, the proper branches of this reasoning vegetable, till the axe of intemperance has lopped off his green boughs, and left him a withered trunk; he then flies to art, and puts on a periwig, valuing himself upon an unnatural bundle of hairs, all covered with powder, that never grew on his head; but now should this our broomstick pretend to enter the scene, proud of those birchen spoils it never bore, and all covered with dust, through the sweepings of the finest lady's chamber, we should be apt to ridicule and despise its vanity.

当大自然送他入世之初，他是强壮有力的，处于兴旺时期，满头的天生好发；如果比作一株有理性的植物，那就是枝叶齐全。但不久酗酒贪色就像一把斧子砍掉了他的青枝绿叶，只留给他一根枯株。他赶紧求助于人工，戴上了头套，以一束扑满香粉但非他头上所长的假发为荣。要是我们这把扫帚也这样登场，由于把一些别的树条收集到身上而得意洋洋，其实这些条上尽是尘土，即使是最高贵的夫人房里的尘土，我们一定会笑它是如何虚荣吧！

The baumanii resistance shows upgrade tendency overall, and the persister increases rapidly as well as multi-drug resistance because of widely use even abuse of antibiotics.

由于抗菌药物的广泛使用甚至过度使用，鲍曼不动杆菌的耐药性呈总体上升趋势，耐药株迅速增加，并出现了多重耐药株。

The lag growth phase, as well as the stationary phase was delayed with the increase of salinity.

随着盐度的增加，三株菌株生长的延迟期增加，到达生长稳定期的时间也相应增加。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。