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Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1/HN的己糖激酶编码基因,构建其原核和真核表达重组质粒,测定其序列,比较它及它推导的蛋白质与恶性疟原虫其他和人之间的差异。

Methods: Minimum inhibitory concentrationvalues of three antibiotics were determined in Mueller Hinton broth by the microdilution technique.

用微量液体稀释法测三种抗生素单用时对31铜绿假单胞菌的最低抑菌浓度,并用微量液体平板棋盘法测出联合用药时对这些菌的MIC,计算FIC指数。

Methods A κ-carrageenan-degrading bacterium AJ5, capable of utilizing κ-carra-geenan as sole source of carbon and energy, was isolated from the intestine of holothurian Apostichopus japonicus by enrichment culture technique.

通过富集培养技术从刺参肠道分离出一卡拉胶降解菌AJ5,该菌能利用卡拉胶作为惟一碳源和能源。

At the same time,the antagonism were confirmed by filter paper method.According to the classification standard of Raper(1965),NO.1 strain is generally in good agreement with the standard of holotype Aspergillus niger V.Tieghen 1867,therefore,we identified and named the type culture as Aspergillus niger xj.

Tieghen 1867的标准形态基本相同,鉴定该菌属于曲霉属黑曲霉群的一黑曲霉,记为黑曲霉xj,并将该菌种保藏在中国典型培养物保藏中心。

The G93 2 strain is different, the homology of this part of sequence with them is only 79.9%, but it ha...

并对G93 2病毒的生物学特性进行了研究,结果与 87A一致。

The homology between them and 6 of 13 foreign trypanosoma were 99%—100%,62% between them and 7 foreign trypanosome strains.

与国外的6锥虫的同源性为99%—100%,与另外7的同源性为62%。

The mutant decreased in growth rate, and lost in pathogenicity to wheat seedling and in homothallism as compared with those of parent isolate.

该突变与亲本菌相比,生长速率下降、致病性和同宗配合能力丧失。

Although the conservative termed secondary homothallism inherit mode and absence of wild resources, the commercial strains encompass extremely limited genetic variation, in this paper we revealed some variations of phenotypic and genorypic characteristics among the tested strains in mycelium growth rate, field performance, esterase isozyme, soluble protein and AFLP fmgerprint.

双孢蘑菇由于自身保守的次级同宗配合遗传方式以及长期野生种质资源的匮乏,造成现有栽培菌之间相似性程度较高,尽管这样,本试验采用不同方法,从菌丝生长速度、农艺性状、蛋白质以及基因组各方面系统地反映了双孢蘑菇主要商业栽培菌间的表型及遗传差异性,为双孢蘑菇种内杂交育种提供了科学依据。

The effects of different host plant on the stability of plasmid in HA 12-1-12 and HA 7-2-2 were also assessed.

进一步考察了其中两个菌HA 12-1-12和HA 7-2-2的质粒在四种宿主体内的稳定性,结果表明HA 12-1-12在不同宿主体内高度稳定,HA 7-2-2却发生了不同程度的变异,得到10个变异

Comparing 20 strains of the PSB in the study, R. azotoformans Y6 was chosen for producing the most lycopene output, which is 8.02×10~(-3) mg/L. Then the effects of pyridine, Triton-x100 Span-20,β-ionone, Isonicotinic acid hydrazide was studied.

利用上述获得的光合细菌类胡萝卜素提取方法和检测方法,对实验室保存的光合细菌菌产生番茄红素能力进行了比较,得到了番茄红素产生量较高的菌R.azotoformans Y6,其番茄红素产量为8.02×10~(-3)mg/L。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。