株

S024 -strain, which was screened from soil, inhibits Rhizotonia cerealis intensely which can cause Wheat sharp eyespot.

S024菌株是从土壤中分离出来的对引起小麦纹枯病的禾谷丝核菌具有较强抑制力的生防放线菌菌株。

Strain, which was screened from soil by Plant Pathology Lab. ofHenan agricultural university, inhibits Rhizotonia cerealis intensely which can cause Wheat sharp eyespot. Based on previous research, it was found that 024 strain showed its inhibitory effect by producing antibiotic.

024菌株是河南农业大学植物病理研究室从土壤中分离出来的对引起小麦纹枯病的禾谷丝核菌有着强抑制力的放线菌菌株。

In conclusion, strains ZW23 and ZW27 are two typical facultative aerobic denitrifiers of high efficiency in the removal of N from water.

菌株ZW23和ZW27是两株典型的高效兼性好氧反硝化细菌，具有重要的应用价值。

We collected eight field data sets of potatoes farms in five years (2004~2008) from Taiwan Seed Improvement and Propagation Station. Besides, generalized linear models were employed to estimate the proportion of the individual plant infected. By mean square error as criteria. optimal group sizes were changed from 20 to 40 plants per group so that the cost was reduced and the testing process was improved efficiently. Not only can the group-testing design advance the accuracy of testing, but it can reach to the purpose of optimization.

本研究收集行政院农业委员会种苗改良繁殖场所栽种共5年(2004~2008)8个马铃薯用间试验资料，并应用广义线性摸式来估计单株马铃薯病毒病感染率，进而以均方误差为标准，来计算每群集最佳之植株数，结果显示可将原来每群集20株检测植株扩增至40株，这不仅能降低成本且不影响检测之精确度，可达到最佳化之目的。

Result The fimbria of isolates and standard strains had a good expression by shaking culture at 37℃ for 24h on TSB medium.

结果］采用TSB培养基，37℃摇床培养24h，分离株和标准株菌毛均获得了良好的表达。

The strain X_6 was a non-flocculence strain with higher fermentation degree and the strain N_1 was a strain with lower fermentation and stronger flocculence.

以发酵度较高的非絮凝性的啤酒酵母菌株X6和发酵度较低、絮凝性较强的啤酒酵母菌株N1为亲本进行原生质体融合。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

The multiple alignment and phylogenetic analysis demonstrated that each genewas a member of a multigene family. 3. The function of 3GT gene was confirmed by Agrobacterium-mediatedtransformation of arabidopsis thaliana. Arabidopsis thaliana was transformed by floral dip method with AgrobacteriumGV3101 carrying expression vector pG3GT. Four transformants were selected for theirgrowth ability on 1/2MS medium containing 50mg/L kanamycin.

马铃薯野生种3GT基因的功能验证为了验证马铃薯野生种3GT基因的功能，构建带CaMV 35S启动子的表达载体pG3GT，转化农杆菌GV3101，用茵液浸泡花序法对拟南芥进行遗传转化，在含50mg／LKan的1／2MS培养基上筛选，得到4个抗性幼苗，转化率为0.13％，对移栽成活的3株进行PCR检测为阳性，Southern blot分析2株表现为阳性并为单拷贝整合。

Genomic PCR of T0 generation plants revealed that the DNAj gene from foxtail millet was integrated into one wheat plant by repeated assay.

对转化获得的703株T0小麦植株提取DNA进行分子检测，有1株小麦稳定扩增到了预期的1 260 bp的目的基因片段。

In fungal diversity analysis, thirty six of 40 clones were identified as Neocallimastix frontalis and two clones as Aspergillus penicillioides and Pacecilomyces sp., repectively. And the other two clones were recognized as uncultured fungi.

在真菌多样性分析中，40个株系中有36个属於 Neocallimastix frontalis 。，二株各分属於 Aspergillus penicillioides， Paecilomyces sp。，另二株则属於未培养的真菌。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。