株

Results 761 strains were isolated from 722 patients (50.1%) of which, 170 (22.3%) were Klebsiella pneumoniae, 130 (17.1%) were Escherichia coli, 89 (11.7%) were Streptococcus pneumoniae, 63 (8.3%) were Staphylococcus aureus, 60 (7.9%) were Hemophilus influenzae and Hemophilus parainfluenzae.

结果 1441例病人中，722例检出细菌共761株，分离阳性率为50.1%，分离菌依次为肺炎克雷伯菌170株(22.3%)、大肠埃希菌130株(17.1%)、肺炎链球菌89株(11.7%)、金黄色葡萄球菌63株(8.3%)及流感和副流感嗜血杆菌60株(7.9%)。

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

Results Five hereditarily stable strains with high lysine production were obtained by four cycles of genome shuffling. A high lysine producing strain, F4-36, was obtained, which produced 16.95 g/dL lysine, 37.1% higher than that of the starting strain.

通过四轮基因组重排成功选育出了5株遗传稳定的高产赖氨酸菌株，其中1株重排菌株赖氨酸产量达到16.95 g/dL，比原始菌株Corynebacterium pekinense 1赖氨酸产量提高了37.14%，比亲本菌株赖氨酸产量提高了17.46％~31.19%。

According to one conserved sequences of phage GIL01 from Bacillus thuringiensis,a pair of primers was designed.With overnight cultivations of 35 B.t standard strains and one fermentative strain MZ1 as templates,PCR results showed that 16 strains,which may be lysogenic strains,produced the product with the size about 750 bp as anticipated.

根据苏云金杆菌(Bacillus thuringiensis， B.t)溶原性噬菌体GIL01(GenBank Accession Number： AJ536703)的同源序列设计一对引物，用35株B.t标准菌株和生产菌株MZ1(血清型H3a3b)的过夜培养物作模板，结果有16株菌株包括MZ1能扩增出大小约为750 bp的预期产物，推测这16株菌为溶原菌。

Methods Targeting the most common mutation types at codon 526 and codon 531 of Rifampiein Resistance Determining Region of rpoB gene from Mycobacterium tuberculosis ,labeled probes （526CAC, 526TAC, 531TCG and 531TTG） were designed to detect 38 Rifampin-resistant clinical isolates with known RRDR sequence, 24 Rifampin-sensitive clinical isolates with wild type sequence of RRDR and 5 nontuberculous mycobacteria isolates, then a method using real-time PCR was established.

方法针对结核分枝杆菌rpoB基因利福平耐药决定区（Rifampicin Resistance Determining Region，RRDR）526密码子和531密码子常见的突变形式设计探针（526CAC，526TAC，531TCG和531TTG），应用已知rpoB基因RRDR区序列的38株利福平耐药临床分离株和24株利福平敏感临床分离株以及5株非结核分枝杆菌菌株建立荧光定量PCR检测基因突变的方法。

Results After 4 hours of test, the non-epidemic strains became positive and the average growth density of the non-epidemic strains was higher than that of the epidemic strains; however, some were still lower than the epidemic strains. In contrast, at the eighth hour oftest, when epidemic strains got positive, they showed higher average growth density.

结果 在甘露醇发酵实验第4小时非流行株出现阳性反应时，非流行株整体生长趋势较流行株迅速，但单个菌株生长速度有快有慢；第8小时流行株出现阳性反应时，其生长高于非流行株。

To control tobacco black shank disease rationally, reduce resistance, 34 strains of Phytophthora parasitica var. nicotianae were isolated from 6 region of Yunnan province and all isolates were determined their resistance to metalaxyl. The results indicated that of all tested isolates, there were 12 high resistance, 5 middle resistance and 17 low resistance isolates respectively.

为合理防治烟草黑胫病，减少抗药性产生，从云南省6个地州分离烟草黑胫病菌菌株34个，就分离菌株对甲霜灵的抗药性进行了测定，结果表明，供试34个菌株中中高抗菌株12个，中抗菌株比例较高5个，低抗菌株17个。

The allomatric scaling for the wet clonal plants including Phalaris arundinacea Scirpus planiculmis Phragmites australis and Phragmites Jeholensis did not show "1/4-power Rule". The variation of the allometric exponents between vegetative and reproductive ramets was mainly due to the different regulation mechanisms in different development stages. Moreover, the variation of the allometric exponents between plantsin wet or drought environments was the result of the long-time divergent response for ramets to moisture environments.

虉草、紧穗三棱草、芦苇和热河芦苇4种湿地根茎植物分株异速生长规律并不表现为&1／4或1／4整倍数&规律，营养株和生殖株异速生长幂值的差异是不同发育类型的分株生长调节机制不同的结果，水生生境和旱生生境分株异速生长幂值的差异则是分株对生境水因子差异长期趋异响应的结果。

The purpose of this study is to screen microorganisms with growth inhibition against plant fungal pathogens. The plate-dilution method was used to isolate the bacteria from salt marshes at Dafeng, Jiangsu. The antifungal activity of the bacterial broth filtrates against 5 phytopathogens was determined using mycelial disk method. The antifungal compositions of four active bacteria were analyzed primarily with chromatography techniques. Two hundred and thirty-five aerobic (92) and anaerobic (143) bacterial strains have been isolated from the coast salt marsh and sixty-five strains, of which 39 are aerobic and 26 are anaerobic showed greater than 30% of inhibition rate against the growth of at least one of the phytopathogenic fungi on petri dish.

为了开发对植物病原真菌生长有抑制作用的药源微生物，本实验采用平板稀释法从江苏大丰盐沼湿地土壤中分离微生物，并通过菌丝块法检测这些微生物代谢产物对5种植物病原真菌生长的抑制活性，进而利用柱层析分析其活性成分；结果共分离得到235株细菌，其中好氧细菌92株，厌氧细菌143株；体外活性测试结果显示有65株至少对1种测试植物病原真菌的抑制率在30%以上，其中好氧菌39株，厌氧菌26株。

Moreover, the damage of ionic etching and phenomenonof dilapidating cell wall could be seen. When implanted dose increased continually, thedamage to cells became more and more serious. Biological characteristics of the original strain ANO_1 and the mutant strain ANO_2were researched. Colonies appeared after ANO_2 had been cultured on standard medium for35h and ANO_1 for 25h.

通过比较原菌株 ANO_1和变异菌株 ANO_2生长特性的研究发现：原始菌株 ANO_1和变异菌株 ANO_2同时接种单菌落于含有麸皮汁的平板培养基中，变异菌株 ANO_2在平板培养基上生长缓慢，35 h 才出现针尖状菌落，而原菌株 25 h 就出现了针尖状的菌落。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。