株

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中，我们在相同条件下，将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells，HEL)和3株传代细胞株：人肝癌细胞(Human hepatic carcinoma 3B cells，Hep-3B)；人肺腺癌细胞(Human lung carcinoma A549 cells，A549)；鼠正常成纤维细胞(Normal mouse fibroblastscells，NIH 3T3)。

An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 ( Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子色谱、Phenyl-Sepharose疏水色谱等步骤获得了凝胶电泳均一的酰胺酶。

A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子层析、Phenyl-Sepharose疏水层析等步骤获得了凝胶电泳均一的酰胺酶。

Results The seven isolates were rod-shaped, Gram-negative, obligate anaerobe, and spores formation was not observed.

7株菌株细胞形态均为杆状，不产芽孢，革兰氏阴性，严格厌氧，在70℃生长良好。

Futher results showed that: the optimum growth temperature for the 4 lactic acid bacteria was 30℃; and the pH 3.5~4.0; all 4 lactic acid bacteria could grow at the medium with 10% NaCl, and the optimum range was 2%~6% They were aerotolerant anaerobe; They showed certain inhibition on E coli..

进一步测试表明：4株乳酸菌在30℃培养时，生长最好；在pH3.5~4.0时生长良好；4株乳酸菌在10% NaCl的环境中均能生长，其中以2%~6%的NaCl浓度内生长最适宜；属兼性厌氧菌；对大肠杆菌产生一定的抑制作用。

Results The pigment is proved to be hypocrellin A.The isolate was determined as the stable anamorphosis strain 0258 of S.bambusicola.

结果从竹黄子实体分离筛选到一株无性型菌株0258，其能产生稳定的竹红菌甲素。

More metabolites could be detected when the TLC was carried out in system II (chloroform∶methanol=9∶1) and system III (butanol∶acetic acid∶water=4∶1∶5) with anisaldehyde test solution.

对每一株菌而言，产生最多产物的发酵培养基各不相同，为了获得尽可能多的次生代谢产物，不同菌株选用的发酵培养基不一样。

Three developing systems were used for chromatography of TLC. UV 254nm, 366nm and anisaldehyde as a chromogenic agent were used for detecting compounds of these strains.

本研究设计4种内生菌发酵培养基，对89株分离自傣药植物且具有高抗菌活性的内生菌及5株潜在放线菌新种进行摇瓶发酵，发酵液的提取物用3种展层系统作薄层色谱展层，用UV(254nm、366nm)和茴香醛试剂进行检测。

Anise oil is a natural spice in Guangxi. Isolation of trans-anethole degrading bacterial strains and trans-anethole biotransformation process were performed using anise oil as the only carbon source. The target strains were expected to produce high value-added nature spice and medicine intermediate by biotransformation of trans-anethole—the main constituent of anise oil. Bacteria were isolated from soil under aniseed trees by enrichment culture.

以广西特色香料资源茴油为碳源，开展了反式茴脑生物降解菌株的筛选工作，并对反式茴脑生物转化过程进行了初步研究，以期获得的菌株能催化茴油中的主要成分反式茴脑，合成高附加值的天然香料或医药中间体。

Cultivating liquids of strain A and B couldcontrol Pepper Anthracnose according to filed test.

化感作用表明：菌株A和菌株B的培养滤液均能够促进辣椒种子的萌发和生长。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。