株

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

Based on morphological characteristics and sequence alignment analysis of rDNA ITS region, 16 strains isolated from diseased plants of Setaria viridis from Beijing, Hebei and Henan, two strains isolated from diseased plants of Chloris virgata and Eleusine indica from Henan, and one strain isolated from diseased plant of Avena fatua from Qinghai, were identified as Bipolaris setariae.

经形态学鉴定和rDNA ITS序列分析，16株分离自北京、河北、河南发病狗尾草的菌株、2株分别分离自河南发病虎尾草、牛筋草的菌株和1株分离自青海发病野燕麦的菌株被鉴定为狗尾草平脐蠕孢Bipolaris setariae。

The full F gene was sequenced and compared with the published sequences of virulent and avirulent strains of NDV in GenBank database.

根据分离株序列与GenBank中NDV相关毒株序列比较分析，结果表明，所分离的6个分离株归属为3个NDV基因型，其中有基因Ⅶd亚型（3株）、基因Ⅵ型（2株）和基因Ⅲ型（1株）。

When the mixture of bacteria strain MH and fungi strain Cladosporium is used, the extent of diesel biodegradation is significantly increased by 80% after five days; Diesel pollutants in aqueous solution can be completely removed by synergistic use of these MH and Cladosporium; The observed synergistic effect is closely related to the aromatics-resistance of the fungi strain Cladosporium, which in turn favors the growth of the MH.

当分支杆菌菌株和枝孢属菌株混合使用时，柴油在五天后的生物降解率高达80%；通过分支杆菌菌株和枝孢属菌株的协同降解作用可完全除去水体中的柴油污染物；协同作用主要涉及到枝孢属菌株对芳香烃的降解而促进了分支杆菌菌株的生长。

After treated by ultraviolet and EMS, a mutant M2-4-4 was produced from Cytophaga MCA-2, with a higher enzyme activity of 66U/ml.

经过多次富集和反复筛选，从海洋环境中筛选到69株具有卡拉胶降解活性的菌株，以产酶活力高、生物学性状优良的MCA-2菌株为出发菌株，进行复合诱变和自然选育，获得了一株酶活显著提高的突变株M2-4-4，发酵液产酶活力达到66U/ml。

The second part of research is analysis of the decolorization bacterial by anaerobic bioreactor, decolour experimental result: To decolour complete for 6 hour have 5 colonies, define for being high decolor bacterial; to decolour complete for 6~36 hour have 20 colonies, define for being medium decolor bacterial; And the person who greater thans 70% of decolouring rate have 25 colonies within 36 hours, define for being low decolor bacterial.

研究第二部份为厌氧脱色槽中之厌氧脱色菌种之分析，初步脱色实验结果为6小时内脱色完毕者有5株，定义为高脱色菌株；6~36小时内脱色完毕者有20株，定义为中脱色菌株；而在6~36小时内脱色率大於70%者有25株，定义为低脱色菌株。

The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bocillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis.

结果：分离得到421株细菌，包括活性菌株54株，其中活性菌株32-11-2-2是一株弱嗜盐菌，具有陆地芽孢杆菌的形态特点，能产生胞外淀粉酶、过氧化氢酶，液化明胶、石蕊牛奶反应阳性，16S rDNA序列分析该菌株与枯草芽孢杆菌同源性达到98%以上。

In our study, 3 genotypies have been respectively demonstrated in UL139 and UL149 for the first time, and the corelations between certain genotypical structure and certain diseases were also proposed; The existence of the 3 genotypes of UL144 was foremost verified in isolates from congenital infants; Furthermore, the UL140, UL141 and UL145 genes were observed to be greatly discrepant to those had been described previously: comparing with Toledo, 231 nt are inserted in UL140, 2 more ORFs are obtained in UL141, and the UL145 ORF moves upstream by 90 nt. Except UL144, the sequences of 18 else genes in clinical isolates were submitted for the first time, and 479 sequences were assigned by GeneBank in total. Relevantly, 9 papers were published.

我们在学术界首次证实了HCMV UL139、UL149两个基因在临床分离株中分别存在三种基因型，发现了其中某个基因型的基因结构与特定来源的分离株存在一定的对应关系；首次在先天感染分离株中验证了UL144三种基因型的存在；首次证实了UL140、UL141和UL145等基因与原先认识的基因结构不同，其中UL140基因较Toledo株增加了231个碱基、UL141产生2个新的基因编码区、UL145 ORF较Toledo株前移90个碱基；首次或最先提交了除UL144基因外其余18个基因的临床分离株序列，本项目组共有479个HCMV相关基因序列被GenBank收录；发表研究论文9篇。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis. The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1.S.agalactiae, 1 S. eguisimilis and 1 S. uberis, and 4 non- streptococus strains were negative. Emm6 was found not only in suppurative hemolytic streptococus, but also in S.uberis.

应用聚合酶链式反应方法对点滴型银屑病患者咽部培养出的19株细菌中的M6蛋白基因进行检测，结果，15株链球菌中6株为阳性，其中化脓链球菌3株，无乳链球菌、似马链球菌和乳房链球菌各1株，4株非链球菌均为阴性。M6蛋白基因不仅存在化脓性溶血链球菌中，在其它链球菌如乳房链球菌中也存在。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。