株

The titres of erythromycin in original strain and recombinant were 3.98 and 5.15g/L, respectively, i.e.

红霉素效价，原始菌株和重组菌株分别为3.98和5.15g／L，相当于重组菌株提高红霉素体积产率约29％。

The total protein content of recombinant was lower than that of original strain by 27%. The titres of erythromycin in original strain and recombinant were 3.98 and 5.15g/L, respectively, i.e.

在两个菌株中生物物质浓度［以总蛋白质表示］存在不同，重组菌株比原始菌株约低27%。

A dwarf mutation 99CDAM with plant height about 85 cm was discovered from a Brassica napus line which had selfed for many years. 99CDAM has good characters of early flowering and rich branches, as well as better yield and quality traits, which can be inherited stably, so 99CDAM has important value in Brassica napus breeding.

在甘蓝型油菜自交多代品系中发现了一个株高85cm左右的矮秆突变株99CDAM，该突变株具有开花早、分枝多等优良特性，产量性状和品质性状较好，各性状都能稳定遗传，具有重要的育种价值。

Among them 3 plants wereselected by the resistance to Ss in adult stage and the result of backgroundanalysis with another 63 PCR markers. So the seeds of the 3 selfed andtestcross were sowed.

成株期抗性鉴定发现其中3株终花期抗性最好，遂将这3株自交和与不育系授粉测交的种子播种，用于下一轮分析。8 在3个单株产生的株系中，有一个株系测交群体无育性分离。

Through the selection of culture medium and the mutant of mycelium that has a high production of PUPAS oil , the oil containing mycelium can be obtained with fermentation, and the dry mycelium contains 42.75挆47.78% oil.

本课题通过研究，选出了深黄被孢霉Mortierellaisabellina株作为摇瓶小试菌株，该株生长快，菌丝多，利用培养基原料能力强，易于形成孢子，生物量49g干菌体/L发酵液，含PUFAs46.61％，其中GLA2.3％。

The cell cultures were monitored by provirus DNA PCR, RT-PCR, reverse transcriptase activity assay and real-time RT-PCR, The results of RT activity and DNA PCR, RT-PCR were positive in the supernatant of cell cultures and viral particles were also clearly observed under electron microscope.

本试验基于代次毒分析结果，选取LTR 变异率较高的U3 区，以驴胎皮肤弱毒疫苗感染性分子克隆株pLGFD3-8 和驴强毒株感染性分子克隆株为父本操作系统进行基因替换，构建EIAV 非编码区强弱毒嵌合的全基因分子克隆，将阳性重组质粒命名为pLGFD9-12。

Go out to choose agree with microzyme of barmy of alcohol of sugar cane juice individual plant, optimized microzyme of two individual plant (of jl2008 and ggfs16) ferment condition, considered to optimize fungus of below the condition two plant to reach its hydrolyze production with sucrose the alcohol when the content that it is a bottom ferments circumstance.

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Due to potential instability of the prophages carrying the stx gene, in this study, the prophage of O157:H7 86-24 strain was cured with serial passages of bacteria, confirmed by PCR and DNA sequencing. The cultural supernatant of O157 prophage curing mutant was inoculated into Vero cells, indicating that the prophage curing mutant of O157 lost the toxigenicity to Vero cells.

由于原噬菌体本身具有潜在的不稳定性，本研究以O157：H7 86-24为始发菌株，将其进行传代培养，筛选原噬菌体消除菌株，对原噬菌体在O157基因组中的整合位点进行分析，并用Vero细胞对原噬菌体消除菌株的细胞毒性进行鉴定。

Four isolates, SP4, SP5, BEG 167 and BEG 141. were selected from the 17 tested isolates. The effectiveness of the isolates was related to ecological attributes such as the number of propagules formed in the soil.

以宿主植物的生物量、产量及吸磷效应作为筛选高效菌株的主要标准，在所用的17个菌株中，选出SP4、SP5、BEG167和BEG141为高效菌株。

In the cells of the fifth generation however, the formation of polyhedra could not be observed.

为了探索获得宿主域扩大的重组病毒的途径，本研究利用G3及BmNPV均能在SpIm细胞中形成病毒多角体的特点，将2株病毒共感染SpIm细胞，经空斑法培养获得一株新的病毒株，其DNA的酶切电泳图谱与BmNPV DNA相似，在前4代感染Se301和BmN4细胞均可形成多角体，但第5次继代感染Se301细胞时，不再观察到多角体的形成。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。