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In order to enhance the trehalose production, Y7 was used for mutation with EMS. Through primary screening and compound screening, strain L61 with high-yielding trehalose and deep neutral trehalase activity was screened.

实验对出发菌Y7进行了EMS诱变处理,通过初筛、复筛,从中选育出一能高产海藻糖同时具有较低海藻糖分解酶活性的菌L61。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35气单胞菌分离进行了脂酶的表型检测和PCR检测,结果分别为83%和100%,特别是嗜水气单胞菌无毒W1和Ah4332也能检测到脂酶活性或基因,表明脂酶在该菌的致病机制中不是决定性因子。

There are not only separate male and female but also trees with flowers of both sexes: ash is "trioecious".

不仅有分离的雄和雌,而且也有两性花的树木;白蜡是&单全异&的。

To study the taxonomy position of two Trypanosoma evansi strains from buffalos in Guangxi and Hubei provinces, the ITS (ITS1-5.8S-ITS2) gene of the T. evansi were amplified by PCR and sequenced.

为研究从水牛分离到的伊氏锥虫广西和湖北的分类学地位,对2伊氏锥虫的核糖体基因内转录间隔区(ITS1-5.8S-ITS2)基因进行了克隆和测序分析。

A study was done to isolate and identify yeasts from apple, which (15 samples) were collected from the North Andre Apple Juice Co. Ltd. The yeasts were isolated with unpasteurized samples in the media of glucose-yeast extract-tryptone media. The identification of yeasts were done with the physiological characteristics and the API 20C AUX system. The Candida spp., Kloeckere spp. and Saccharomyces spp.

利用酵母粉-葡萄糖琼脂培养基对15个批次苹果原料样本中的酵母菌进行分离,并用API20C AUX菌种鉴定系统进行鉴定,得到的25酵母菌分别属於假丝酵母菌属、酿酒酵母菌属和克勒克酵母菌属的6个种:克柔/平常假丝酵母菌 13,酿酒酵母菌 5,克勒克酵母菌属酵母菌Kloeckera spp。

Coli. Methods Among 95 unrepeated strains isolated from clinic, 38 positive strains tested by ESBLs confirmatory test were collected from HAI and CAI subjected to detection for drug resistant gene TEM, SHV, CTX-M-1 and for disinfectant resistant gene qacE△l-sull by polymerase chain reaction.

收集从临床标本分离的大肠埃希菌95,经ESBLs确证试验将其中38阳性菌分为医院与社区获得性感染2组,用聚合酶链反应检测耐药基因TEM、SHV、CTX-M-1以及耐消毒剂基因qacE△1-su l1。

The bactericidal, fungicidal and virucidal activity of chlorine dioxide (CIO2) against 79 bacteria, 31 yeasts and 4 viruses were evaluated under clean and dirty conditions. The safety of CIO2 to chickens given by drinking water or skin spraying was also evaluated.

本试验进行二氧化氯(CIO2)在乾净水中和污浊水中分别对79细菌和31酵母菌和4病毒的杀灭力及以饮水或喷洒方式投予鸡只CIO2之安全性评估。

A total of 442 BC〓F〓 and 9 BC〓F〓 plants were obtained by backcrossing F〓 and BC〓F〓 to 02428, the chromosome number of 42 BC〓F〓 and all BC〓F〓 plants so far examined varied from 24 to 38, from which 21 plants with 2n=25 and 21 diploid plants were isolated.

进一步用02428对F2和BC1 F1回交共获得442BC1F2和9BC2F1,已确定其中42BC1F1及所有BC2F1植的体细胞染色体数目,其变幅为24-38,2n=25及2n=24的植各有21个。

Results By MSHA/MRHA, the fimbriae of 4 of the 5 isolates were identified as type Ⅲ, and the rest one as undefined type. However, by PCR, all the fimbriae of 5 isolates were identified as type Ⅲ.

结果 MSHA/MRHA方法鉴定其中的4菌具有Ⅲ型菌毛,另外1无法鉴别,而PCR方法鉴定5菌均具有Ⅲ型菌毛。

The Etest is an altenmative to agar dilution method for gonococci susceptibility testing to ceftriaxone,penicillin and ciprofloxacin due to its rapid,effectiveness and significant relationship with agar dilution method.

来源实验菌均从我所STD门诊患者的泌尿生殖道分泌物中分离得到,所有菌经革兰染色、氧化酶试验和糖发酵试验证实为淋球菌传代一次,洗于脱脂牛奶中,于- 78℃低温冰箱冻存备用。2 。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。