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Results Among 70 strains of Staphylococcus spp., 56 strains were positive Staphylococcus coagulase gene with PCR; the same 56 strains were also positive with latex agglutination test; 54 of which were positive with tube test method, 2 strains were false negative; strong positive were detected with slide method, all these 56 strains were identified as Staphylococcus aureus by VITEK-Ⅱ automatic analyzer.

结果 70株葡萄球菌属细菌中，PCR法检测葡萄球菌凝固酶基因56株为阳性；Slidex Staph Plus与PCR法相对应的56株为阳性；试管法检测与前2种方法相对应的54株为阳性，2株为假阴性；玻片法为强阳性；VITEK-Ⅱ全自动分析仪鉴定56株为金黄色葡萄球菌

Results Isolating 1 358 pathogenic bacteria from 4 665 samples,which include 1 217 bacilli and 141 fungus.Among the 1 217 bacilli,Gram negative bacilli and Gram positive coccus were 800(65.7 %) and 417(34.3 %) respectively.

结果4 665份标本中，分离到1 358株病原菌，其中细菌1 217株，真菌141株；1 217株细菌中，革兰阴性杆菌800株，占65.7%；革兰阳性球菌417株，占34.3%。

Strains could inhibit conidium germination, 5 strains made the pathogen grow vegetably and 9 strains inhibited its tube growth.M.grisea produce several swollen cells on the sites of the appressorium differentation and lost the ability to infect onion epidermis treated with the culture supernatant of h23 strain.

结果如下：获得21株对稻瘟病菌附着胞形成具有拮抗作用细菌菌株，其中3株诱导孢子在附着胞分化位点产生囊状物；其中4株抑制孢子萌发；5株使孢子呈营养生长；9株抑制芽管生长。

Seven bacterium fungus and four microzyme fungus were isolated from tuber mustard by plate counting. It showed that two bacteria fungus will induce tuber mustard corruption in counterevidence experiments.

采用平板划线法从榨菜中分离得到7株细菌菌株和4株酵母菌菌株，经过反证试验，确定其中的2株细菌菌株是引起榨菜腐败变质的微生物。

Twenty two strains(14 bacteria and 8 actinomyces strains) of antagonistic microorganisms against Fulvia fulva were isolated from vegetable field soil.

从蔬菜地田间土壤中筛选出对番茄叶霉病具拮抗作用的22株菌株，其中14株细菌，8株放线菌。孢子萌发抑制试验表明，细菌X14菌株和放线菌F15菌株的发酵液及其离心后上清液对番茄叶霉病菌孢子萌发抑制率均达100%。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

The antibiotic resistance of staphylococcus aureus was detected by K-B method. Of 33 staphylococcus aureus stains isolated from food, 28 were resistant to penicillium and ampicillin, the resistance rate was as high as 84.8%.21 stains were resistant to carbenicillin, the rate was 63.6%.5 stains were detected resistant to methicillin, which took up to 15.2%. The stains also were resistant to cephalosporin antibiotics, aminoglycoside, microcline antibiotic and tetracyclines in different levels, all strains were sensitive to vancomycin.

结果 33株食品中分离的金黄色葡萄球菌，有28株对青霉素和氨苄西林耐药，耐药率高达84.8%；21株对卡比西林耐药，耐药率达63.6%；检出了5株耐甲氧西林的菌株，耐药率为15.2%；对其他头孢类抗生素、氨基糖苷类、大环内脂类、四环素类等都出现出不同程度的耐药；所有菌株均对万古霉素敏感。

In 42 ACME-arcA-positive isolates, 8 isolates harbored SCCmec V, 8 isolates harbored class C1 mec complex and ccrAB_, 22 isolates harboring class C1 mec complex and 4 isolates harboring class C2 mec complex were negative for all known ccr allotypes.

本研究在ACME-arcA基因阳性菌株中发现整合酶基因ccr的一种新亚型ccrAB_(SHP.42株ACME-arcA基因阳性菌株，8株携带V型SCCmec，8株携带C1型mec基因复合体和ccrAB_，剩余26株携带C型mec基因复合体和未分型ccr。

Ata28 strain exhibited marked suppression of Tbs3 strain which is the pathogen of tobacco brown spot on PDA plates, and the Ata28 strain had the good control efficiency of tobacco brown spot.

采用紫外线诱变Ata28菌株时，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，20s，30s诱变；通过平板对峙培养，筛选出15个突变菌株在PDA平板上可明显抑制Tos3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.4 mm；在温室控病中，获得了对烟草赤星病有较好防效的突变菌株Uv-126，烟苗经Uv-126菌液处理3d后接种Tos3的防治效果达52.2％，比出发菌株Ata28的防治效果好。

Ata28 strain was mutated by UV with the most optimum mutant time, including 10, 20, 30s, that was chosen by the trial tests, considering the lethality rate and positive mutation rate of Ata28; it was found that 15 mutant strains were shown antagonism against Tbs3 strain by antagonism test on PDA plate, inhibition zone of mutant strains exceeded the original strain Ata28, and the maximum of inhibition zone reached 9.4mm; the mutant strain Uv-126 was found most effective in tobacco brown spot control and a rate of efficiency control reached 52.2% on tobacco plants three days after bacteria spraying.

采用硫酸二已酯诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间20min，40min，60min诱变；通过平板对峙培养，筛选出17个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Am28，抑菌带最大值达10.5 mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Des-66、Des-50，烟苗经Des-66、Des-50菌液处理3d后，接种Tbs3的防治效果达52.0％、56.6％，比出发菌株Ata28的防治效果好。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。