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A highly effective screening model was built, in which enrichment was conducted by adding a definite amount of cyanide into the innutrient medium. Forty-nine kinds of cyanide-resistant strains were isolated from twenty samples of soil, among which twenty-nine kinds of strains showed the cyanide-degradation ability and the bacterial strain numbered DC02 was the best.

构建筛选模型,通过施加选择压力,即在一相对贫乏的培养基中加入一定量的氰化物来进行富集,从20份土壤中筛选出49耐氰菌,29具有降氰活性,其中菌DC02降氰效果最佳。

It is shown that G gene has the highest speed during the evolution and has uniqueinquilinous body in order to escape immune pressure from inquilinous body byanalyzing phylogenic trees of five genes in the whole genome. In the evolution of Ggene, DRV and MRV are in different groups. DRV, Chinese vaccine isolated used inhuman and Japanese vaccine isolated used in human are in the same group. MRV,CVS and street viruses isolated from dogs are in the some group. It is shown thatDRV is vaccine isolated compared with phylogenic trees of other genes, and it istestified previous conclusion that unqualified vaccine used for human was used nearthe infected beer cote resulted in spotted deer infected.

对狂犬病毒基因组5个基因的分子进化树分析表明,为了逃避宿主的免疫压力,G基因的进化最快速,而且具有宿主的特异性,在G基因的进化中,DRV和MRV分别位于不同的组群,DRV与中国人用疫苗和日本疫苗在一个组群,MRV与CVS以及犬脑分离的街毒归在一个组群,结合其他基因的进化树表明,DRV与疫苗具有较近的亲缘关系,从分子水平验证了以前对DRV流行病学调查得出的推测,在狂犬病疫情发生的鹿场附近曾使用过人用狂犬病疫苗,导致梅花鹿感染狂犬病毒。

Screening 37 strains which are of insecticidal activity from 2251 strains, the rate is 1.2%.

本论文通过微型平板法从2251微生物中筛选出具有杀虫活性的菌37,阳性率为1.2%。

After freezing and thawing,the ability for itaconic acid formation of Asp.terreus AS 3.2811,AS 3.2822,...

所测定的液氮保存8菌种的生理活性,除两糖化酶活力稍有降低外,其它菌没有明显的变化。

In contrast to the RplJ, Opa, H.8 antigen, and Laz, which were detected in all seven strains, Opc was only detected in one strain confirming the specificities of the antibodies towards the denatured proteins.

结果在7都可侦测到RplJ、 Opa、H.8以及Laz,但只有一菌表现Opc,证明抗血清对变性后的NM表现目标蛋白质具有特异性。

This study has discussed that Lactic acid bacteria are isolated from 752 samples of milk and other dairy products, collected from 500 household in pastoral areas in Inner mongolia, and its biological properties are analyzed and studied.765 strains of lactic acid bacteria, including 28 species of Lactobacellus,2 species strains of Bifidobacterium, 9 species strains of Enterococcus, 3 species strains of Lactococcus, 1 specie of Streptococcus, 6 species of Leuconostoc,1 specie of Pediococcus , are identified.

从代表内蒙古牧区不同地域特点和独特生态环境的9个盟市18个牧业旗500多户牧民家庭,以目的采样和层次分类随机采样相结合的方法,共采集鲜乳及乳制品样品752份,分离到947乳酸菌,并用形态、染色、培养特性及生化反应等实验对其进行了鉴定,有765实验菌分别归于7个属50个种和亚种,其中归于乳杆菌属的有28个种和亚种;归于双歧杆菌属的有2个种;归于肠球菌属的有9个种;归于链球菌属的有1个种;归于乳球菌属的有3个种和亚种;归于明串珠菌属的有6个种和亚种;归于片球菌属的有1个种。

Convey albumen immunity with rarefied LTb 8 Zhou Ling BALB/c rat 3 hind, after taking lienal cell and cell of SP2/0 bone myeloma to undertake confluence, undertake filtration with indirect ELISA and finite and attenuant law, obtain 4 individual plant to be able to stabilize the individual plant of cross tumour cell that secretes antibody of Chan Kelong of LTb of bacili of bowel of the Chinese People's Anti-Japanese Military and Political College, antibody of odd clone of this individual plant names for 1F2, 2C2, 3D6, 4H6 individual plant.

用纯化的LTb表达蛋白免疫8周龄BALB/c鼠3次后,取脾细胞和SP2/0骨髓瘤细胞进行融合后,以间接ELISA和有限稀释法进行筛选,获得4能稳定分泌抗大肠杆菌LTb单克隆抗体的杂交瘤细胞,该单克隆抗体命名为1F2,2C2,3D6,4H6

Eight lipase-producing bacterium strains were screened from 40 cold-adapted strains, these strains isolated from the mud of Parece Vela sea basin in the Pacific Ocean of 5010m deep, of which two similar strains have the highest lipolytic activity and are positive to olive oil.

通过三丁酸甘油酯平板法从太平洋帕里西维拉海盆5010m的底泥中共筛出八可产脂肪酶的菌,其中的两生理生化特征极其相近的菌脂肪酶活性最高,并且对橄榄油平板产生荧光。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌的过夜培养液与指示菌ZK1(血清型H25)混匀并在双层琼脂上培养12 h后出现了滴度为2×107 pfu的噬菌斑,从而证明生产菌MZ1确为溶原菌;又以GIL01同源性较高的其它四个基因设计引物,分别以MZ1的培养物及其溶原性噬菌体基因组为模板进行PCR扩增,结果两者得到几乎一致的带型,进一步证明了生产菌MZ1为溶原菌,说明利用PCR法检测鉴定苏云金杆菌的溶原性是可行的。

Of planted seedlings and conserved seedlings/saplings, 51.6% and 60.7% survived respectively. Thirteen months later, the seedling heights of Ficus septica, Mallotus repandus, Maacaranga tanarius, Melanolepis multiglandulosa, and Broussonetia kaempferi, and those of Hibiscus tiliaceus, Acacia confuse, and Mellettia pinnata increased more than 130 cm and 50 cm, respectively. The densities of the planted seedlings and conserved seedlings/saplings were 1610 and 680 per hectare, respectively. On the other hand, the density of Leucaena seedlings significantly decreased; and the height of Leucaena stayed steadily during the 13 months.

试验分经银合欢药剂注射处后,采用原生树苗下栽植及保天然新苗木方式进相旧,期间经过2次植栽周边除草抚育作业,栽植苗木及天然新苗木在13个月后之存活分别为51.6及60.7%,密分别为1610/ha及680/ha,其中果榕、扛香藤、血桐、虫屎、构树之苗高平均生长在130 cm以上,黄槿、相思树、水黄皮之苗高平均生长在50 cm以上;相对的,银合欢新幼苗在分经药剂处前之平均高为12.3 cm,密为236,000/ha,药剂处后13个月,幼苗平均高为15.0 cm,幼苗密低至43,000/ha。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。