株

Biological characteristics of the avirulent strain 061, 61 - 2 and 61 - 3 isolated from the roots of glycine max inoculated with the avirulent strain 061 were studied.

对鼠疫弱毒株061及用该株菌接种大豆后不同时期从大豆根中分离的61－2、61－3株的某些生物学特性进行比较研究。

The 19 strains of bacilliform bacteria were identified by biochemical tests, part of their 16S ribosomal DNA were amplified, sequenced, and phylogenetically placed.

分离得到得19 株乳杆菌通过菌落、菌株形态观察以及多项生理生化等常规鉴定，并且通过16S rRNA 基因序列测序鉴定，确定这19 株乳杆菌分属于乳杆菌属内专性同型发酵类型中三个不同的种。

China, by using phylogenetic analyses based on gltA gene sequence comparisons. Methods The 32 variants of Bartonella isolated in Yunnan Province and published in GenBank from 1999 to today were taken as the target strains.

用云南省1999年至今分离到的并已在GenBank中公布的32个巴尔通体变异体为目标株，利用网上核酸序列比对程序BLAST进行核酸序列同源性搜索，调出相似性最高且有效发表种典型菌株为对比株，进行比对和系统发育分析。

Methods the 32 variants of bartonella isolated in yunnan province and published in genbank from 1999 to today were taken as the target strains. the most closely related typical strains searched by blast in genbank with sequencing program were taken as comparative stains and their glta gene sequences were compared each other.

用云南省1999年至今分离到的并已在genbank中公布的32个巴尔通体变异体为目标株，利用网上核酸序列比对程序blast进行核酸序列同源性搜索，调出相似性最高且有效发表种典型菌株为对比株，进行比对和系统发育分析。

Objective To investigate the phylogenic diversity of Bartonella in Yunnan Province,P.R.China, by using phylogenetic analyses based on gltA gene sequence comparisons. Methods The 32 variants of Bartonella isolated in Yunnan Province and published in GenBank from 1999 to today were taken as the target strains.

目的 基于gltA基因序列的系统发育分析对云南巴尔通体的样性进行分析方法用云南省1999年至今分离到的并已在GenBank中公布的32 巴尔通体变异体目标株，利用网上核酸序列比对程序BLAST进行核酸序列同源性搜索，调出相似性最高且有效发表典型菌株为对比株，进行比对和系统发育分析。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

In this paper, an efficient and easy biological screening method based on the sensitivity to lovastatin of Neurospora crassa was built to deal with the mutant strain rudely by UV mutation. A high-level strain was screened in the shaking flask culture incorporating with TLC and HPLC. A comparison between this two methods also was originally discussed while identifying dyeing using bromophenol blue as the better treatment of TLC, and offering a kind of simple, practical, fast and economical determination qualitatively and quantitatively.

本文有鉴于此，通过紫外诱变处理，并基于粗糙脉孢酶对洛伐他汀的生物敏感性，对诱变菌株进行了初筛；同时，采用摇瓶培养，结合TLC和HPLC的分析方法，复筛出一株高产菌株；对复筛过程两种分析方法分别进行了初步探讨，比较其优劣，确定TLC法经溴酚兰显色剂显色处理为较佳方式；从而，建立了一套简单、实用、快速、经济且有效的洛伐他汀生产菌诱变筛选方法。

A bacterial strain ADZ1 that demonstrated higher efficient in the aerobic denitrification was isolated from the sewage of urban rivers on the selective medium, Bromothymol Blue, after enriched in the shaking flasks. The reduction efficiency for nitrate and the total nitrogen reached 93.1% and 34.7% within 48 hours respectively. Sequence of 16S rRNA suggested that strain ADZ1 belongs to the genus of Pseudamonas, and further was identified as Pseudomonas putida by VITE K?

采用富集培养和BTB平板法从城市河道污水中筛选、分离获得了一株高效的好氧反硝化菌株ADZ1， 48 h内对硝酸盐的降解率为93.1%，总氮的去除率为34.7%。16S rRNA测序及系统发育分析结果表明该菌株属于Pseudamonas sp。，经VITEK？

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征：①形成英膜，②37℃3天内生长试验皆为阳性，③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落，④尿素酶试验96h皆呈阳性，⑤CGB培养基未见蓝色菌落生长，⑥ID32鉴定板可以鉴定到属，种的鉴定结果各不相同，⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种，⑧血清型仅见A和AD二型，以A型为多数(83.33%)，⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Roots of five species of Calanthe and two species of Phaius from north, midland and south of Taiwan were collected for the isolation of orchid mycorrhizal fungi. Total for 104 isolates of fungi were isolated. They are identified respectively by the traditional method or DNA sequence. Results showed thatTrichoderma and Mucor were most often isolated fungi. The DNA sequence results showed that only Xylaris was most common fungus.

由台湾北、中、南四处地方采集五种原生根节兰及二种原生鹤顶兰，进行此等原生兰花根部菌类分离，共分离出104株代码真菌菌种，分别再以菌落、菌丝和孢子等传统形态特徵鉴定菌种，结果发现以木霉菌及毛霉菌属内之菌株占优势，而利用核酸序列比对之鉴定结果则以炭角菌属内之菌株占多数。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。