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In order to find new kinds of antibacterial agents, a primary screening model has been set with the DNA gyrase B subunit as a target, and then the active compounds of positive strain were purified. From Mycobacterium phlei, we isolated a novobiocin supersensitive mutant M.phlei SS12 and a resistant ~mutant M.phlei RR11 with NTG and UV.

实验采用Mycobacteriumphlei为出发菌株，利用NTG和紫外线复合诱变得到新生霉素特异性的超敏菌株M.phleiSS12和耐药菌株M.phleiRR11，建立了以DNA回旋酶B亚基为靶点的抗结核药物的筛选模型；并应用此模型对1600株真菌和100株放线菌发酵液进行了初筛，获得了38株阳性菌，阳性率为2.4%；对阳性菌株3277的发酵液进行了提取分离，得到X1和X2两个活性化合物。

Unsurprisely,These three microorganisms decreased as the storage time increases.84 strains lactobacillus were isolated during the detection period in the pickled gingers stored in low temperature.By physiology and biochemistry methods,32 strains L.plantarums,6 strains L.brevis,2 strains Lacto...

低温保存泡姜在整个检测期内，共分离到84株典型乳酸菌，经生理生化实验鉴定出植物乳杆菌32株、弯曲乳杆菌7株、短乳杆菌6株、肠膜明串珠菌属5株、乳球菌属2株、乳杆菌属32株；酵母菌分离鉴定出3个属，主要为酵母属以及少量的假丝酵母属和汉逊氏酵母属；只在保存的前2d检测到个别的青霉属和毛霉属；在整个检测期内，没有分离到大肠杆菌。

The character of flower colour and high nicotine conversion inherited steadily,the average nicotine content of their offsprings decreased,while nornicotine content increased significantly.

通过对发现的5株白肋烟B37花色突变株后代的连续观察与研究，结果表明，5株白花突变株当代均为高烟碱转化株；随着世代的增加，白花后代群体花色遗传稳定，高转化率性状更趋于纯合；烟株群体的平均烟碱含量明显下降，降烟碱则明显上升，烟株群体生物碱表现为以降烟碱为主。

There was significant allomatric growth of Phalaris arundinacea and Phalaris arundinacea between ramets height and biomass or constituent biomass, between ramets biomass and constituent biomass in wet and drought environments. And the relationship between ramets biomass or height and ramets quantitative characteristics also followed allomatric growth for Phragmites australis and Phragmites Jeholensis in wet and drought environments.

水生生境和旱生生境生长的虉草和紧穗三棱草，分株高度和分株生物量、组分生物量以及分株生物量和组分生物量间存在着极显著的异速生长关系；水生生境和旱生生境生长的芦苇以及水生生境生长的热河芦苇，分株生物量和分株数量性状以及分株高度和分株数量性状间存在极显著或显著的异速生长关系。

Soaking seed of rice and wheat with zymolytic liquid of Trichoderma harziaiarum, Trichoderma DWC and Trichoderma viride, comparing with germinate ratio, germinate force, root length, bud length and ratio of root to bud, the results show that 50 times dilute zymolytic liquid of Trichoderma harziaiarum has a relatively notable promote role for germinate of seed .The effect of Trichoderma viride to germinate of seed is not evidence. Trichoderma DWC for seed sprout has certain inhibition.

据此，采用哈茨木霉作为出发菌株，通过离子束诱变，从大菌落株中筛选出促进生长的高产突变株——h-13菌株，通过对用该菌株发酵液处理后的水稻及小麦的种子的各项生理生化指标的测定，发现该菌株比其它菌株更能促进水稻和小麦的活力和发芽率，三天时小麦的总根长为3.03cm，比对照的1.42cm的两倍还多，并且能够明显提高种子发芽后的淀粉酶和蛋白酶的活力，其活力都是对照的两倍左右。

Discover in Ping Yuanxin Su Tieye of lake of celestial being of 10 individual plant gives birth to plant; Bai Leshu to be in those who join an administrative division to plant among them group of amounts amount to 160 individual plant, give priority to; to be in with seedling benevolence change fine-toothed comb 3 pointed fir amount to; of 300 individual plant to make the same score Yuan Banfeng carry on one's shoulder to Ren Douxin of; of 100 individual plant sees Yu Qingxin is mixed only then promote, peace of; of above of 1000 individual plant, river source and broadleaf plant mountain discover the amount Su Tie brake is planted group, aggregate; of hundreds individual plant only then allow new discovery tree of silver-colored magpie of 57 individual plant.

其中在平远新发现10株仙湖苏铁野生植株；伯乐树在连州的种群数量达160株，以幼苗为主；在仁化篦子三尖杉达300株；平远半枫荷有100株；任豆新见于清新和始兴，数量1000株以上；和平、河源和蕉岭发现苏铁蕨种群，合计数百株；始兴新发现57株银鹊树。

By the pot experiment in nitrogen-free sand, the difference of the parental strain and marked strains with cfp gene on three cultivars was tested. One marked strain which was not significantly different with the parental strain for the competition stady with soil indigenous strains under field conditions .

通过无菌砂培试验，进行了标记菌株与出发菌株在三个品种上的差异性检测，选出一株在瘤数、植株干重、叶绿素含、全氮含量等各方面指标与出发菌株最为接近的标记菌株用于控制条件下的田间小区试验，比较标记菌株与土著菌株在三个品种上的竞争结瘤能力，结果表明

The illumination is of no effect on the germination rate of oospores of Phytophthora sojae.30 single-oospore isolates obtained from the test strain of Phytophthora sojae Ps-H2 (Race 1 ) were evaluated using hypocotyl-inoculation method for race phenotype on soybean cultivars. One clone phenotype ( race 1 )was selected randomly for further isolation and virulence identify .

将大豆疫霉菌供试菌株Ps-H_2(生理小种1号)每代挑取30个单卵孢株，进行生理小种鉴定，随机挑选1株1号生理小种的单卵孢株继续进行单卵孢株分离和毒性鉴定，连续两代试验结果表明大豆疫霉菌原始菌株并朱出现毒性分化，表明原始菌株即为1号生理小种的毒性纯系。

To control tobacco brown spot disease rationally, reduce resistance to fungicide and reduce pesticide pollution, 96 strains of Alternaria altemata were isolated from 11 region of Yunnan province and all isolates were determined their resistance to dimethachlon. The results indicated that of all tested isolates, there were 28 high resistance, 29 middle resistance and 29 low resistance isolates respectively.

为合理防治烟草赤星病，减少抗药性产生和农药残留，从云南省11个地州分离烟草赤星病菌菌株96株，就分离菌株对菌核净的杭药性进行了测定，结果表明，供试菌株中高抗菌株28个，中抗菌株39个，低抗菌株29个。

In this study, as a original strain, antagonistic bacterium Ata28 was mutated by UV, UV+LiCl, DES and Microwave irradiation; the very goal of the research is to improve the control efficiency of Ata28 strain against tobacco brown spot and obtain strains advanced both in antagonism and biological characteristics, further provides theoretics and practical experience to the production.

采用紫外线和氯化锂复合诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，15s诱变；通过平板对峙培养，筛选出8个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.7mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Uv1-190、Uv1-199，烟苗经Uv1-190、Uv1-199菌液处理3d后，接种Tbs3的防治效果达51.3％、54.9％，比出发菌株Ata28的防治效果好。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。