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A lysogen with vt2 gene was obtained after transfecting MC1061 by VT2 phage SHФW1 isolated from sewage.

将噬菌体分离SHφW1感染MC1061后,经PCR鉴定获得一溶源菌(MC1061/SHφW1)。

The results showed that the optimal temperature for the growth of BY-3 was 30~ 40℃. And the optimal pH was 6~6.5. Besides, the optimal cultivated time was 36~52 h. The favorite carbon source included maltase, sucrose, mannitol, glucose, lactose, and so on.

拮抗菌BY-3菌的研究 2.1 拮抗菌BY-3菌生长特性的测定本研究确定了不同的温度、pH、营养条件及盐浓度对菌BY-3生长的影响,并对其生长曲线作了测定。

Phylogenetic amalysis of the long terminal repeat and env gene of HTLV-I gave a phylogenetic classification of HTLV-I genotypes into five major molecular subtypes~ Cosmopolitan, Japanese , West African , Central African , and Melanesian.

为了解我国HTLV-Ⅰ流行区毒的主要基因型别,本研究从福建莆田地区克隆出6HTLV-Ⅰ的env基因和一tax基因。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离和云南省盈江县农场CYJ/YN 分离基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增,将扩增产物分别经EcoRI 和KpnI 双酶切后,回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体,按Sanger 双脱氧链终止法进行DNA 序列测定,并与MAD20、K1 和Wellcome 原型基因进行同源性分析比较。

Staphylococcus epidermidis (25.0%), and Micrococcus luteus (25.0%), which were all susceptible to cefazolin, chloramphenicol and enrofloxacin.

2与2,且这些菌对多数抗生素皆具敏感性,以cefazolin、chloramphenicol与enrofloxacin等抗生素敏感性较佳。

A strain designated S7 which was isolated from the traditional dairy products was obtained using the agar-diffusion method employing Micrococcus flavus as an indicating strain.

以从传统乳制品中分离得到的7乳酸菌为出发菌,应用琼脂扩散法,以黄色微球菌为指示菌,筛选到了一具有较大抑菌作用的乳酸菌。

Aeromonas hydrophila BA6 was identified as a nonpathogenic agent,while the other seven strains (BA1,BA3,BA10,BAH,BA15,BA16 and XA23) were pathogenic. However,there would be difference in virulence among the strains of the same or different species.

结果表明嗜水气单胞菌BA6为非致病,其余7嗜温气单胞菌为致病,但细菌的毒力大小在不同表型种间和不同菌间都存在差异。

Experimental results indicated tobacco seedlings became more vigorous while leaves exceeding 5cm were cut by 1/3, showing more obvious overcompensation responses, and these seedlings grew quickly after transplanted, there quality and production value were increased.

试验表明,剪叶时剪去5cm以上叶片的1/3有利烟苗产生超越补偿效应,可明显提高烟苗的成苗素质,促进烟的早生快发,提高烟叶的烤后产值和质量;剪叶程度增加,明显降低烟苗成苗素质,抑制烟的生长;不剪叶则导致烟严重早花。

Isolates CNR-1 and CNR-2 were from Brassica napus; CNA-1and CRW-1 from Rhododendron simsii and Oxalis corymbosa respectively. All isolates grew well in R-2 liquid medium and exhibited contractive movements. The colonies of all isolates were circinal and grain-like in solid medium. Through electron microscopy, all isolates exhibited helicity during their growth phase. All isolates could pass through a 0.22 μm filtrate membrane and resist to penicillin (2000 U/mL). They must grow in medium with serum.

这4螺原体在R-2液体培养基中生长良好,都能通过孔径为0.22 mm的微孔滤膜;在R-2固体培养基上呈圆形或颗粒状菌落,菌落直径约50~600 mm;在生长的某个阶段可呈典型的螺旋状,菌体直径为37.04~370.40 nm,长度约0.89~11.88 mm;它们都能利用葡萄糖作为碳源,不能利用尿素;在不含胎牛血清的R-2培养基中,它们都不能生长;菌CNR-1、CNA-1能强烈代谢精氨酸,而CNR-2和CRW-1不能代谢精氨酸;在氨苄青霉素钠浓度高达2000 U/mL的R-2培养基中,分离菌生长良好。

The present study investigatated the effect on the diversification of weed community, including weed abundance, dominance, evenness, biomass, and mean height after mulching straw and intercropping white clover in the tea plantation from 2005 to 2006. The results were as follows: 16 families and 31 species were found in total. In April, the dominant species characterized with slow growth, lower biomass, and less than 30 cm height; in July those who grow faster with higher biomass and height became dominant; In September, these malignant communities reduced, while the oxalis and diffusa characterized with lower biomass and low height gradually increased. In the three seasons, the weed abundance, community diversity and evenness increased, and the dominance, the biomass and average height of weeds remarkably reduced after intercropping white clover and muching straw.

结果表明:茶园共有杂草16科31属31种,春季(4月)以生长速度较慢、生物量小、高在30 cm以下的杂草为主,夏季(7月)以生长快、植高和生物量大恶性杂草为主,秋季(9月)恶性杂草开始减少,酢浆草、白花蛇舌草等生物量小、高矮的杂草逐渐增多;稻草覆盖处理茶园春、夏和秋季3个观测时期杂草物种丰富度、多样性、均匀度等指数均高于清耕对照,优势集中性指数低于清耕对照;间种白三叶草处理春、夏和秋季3个观测时期杂草总数量和生物量均显著低于清耕对照,夏、秋季杂草平均高显著低于清耕对照;稻草覆盖和间种白三叶草处理春季茶芽重和产量显著高于清耕对照,鲜叶中游离氨基酸含量高于清耕对照,茶叶感官评审得分高于清耕对照处理。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。