株

Except S1, the homology between D2-04 and other type 2 viruses was higher than 94%.

D2-04株除与S1株的同源性略低外，其余株的同源性均在94%以上。

Bacterial strains were isolated from humus mud by employing the dilution methods. Seven cellulose-degradation Strains marked as B-37, B-35,B-31, B-25, B-17, Z-a, and Z-b were selected according to the hyaloid rings on the medium of carboxymethylcellulose sodium Congo red and their efficacy of decomposing filter paper. Z-b had the strongest ability in decomposing filter paper among the seven strains and 16S rDNA analyzed indecated that it was Stenotroplaomnonas maltophilia owning to 99.8% homology. Comparing clony size of the seven strains in media with different carbohydrates and inorganic salt, we found that the potato extract and inorganic salt which contained CaCl2 0.20, MgSO4 1.25, NaCl5.00,(NH4) SO4 1.30, KH2 PO4 1.35, FeSO47H2O 0.015, Na-EDTA 0.02 were suitable for the bacteria growth.

用稀释法从腐殖泥中分离菌株，根据它们在梭甲基纤维素钠-刚果红培养基上的透明圈直径及对滤纸的崩解能力，获得7株纤维素分解细菌（编号：B-37、B-35、B-31、B-25、B-17、Z-a和Z-b），其中菌株Z-b的纤维素崩解能力最强，分子鉴定结果表明它与Stenotrophomonas maltophilia的16S rDNA序列有99.8%的同源性，初步确定为嗜麦芽窄食单胞菌。

A high-yielding mutant Rhizopus arrhizus R4 producing γ-linolenic acid was successfully obtained when the original strain Rhizopus arrhizus R0 was treated by UV,and by the screening methods of resisting maleic hydrazide.

以少根根霉R0为出发菌株，利用紫外诱变结合失水苹果酰肼筛选的方法，选育出突变株R4，摇瓶培养菌体油脂含量35.55%，其中GLA占油脂的12.5%，比原始菌株油脂含量提高了93.94%，GLA提高了276.5%。

Result Four bands in the DGGE profiles of total bacterial DNA of Tibetan kefir were not recovered by cultivation.

DGGE指纹图谱显示混合菌群中细菌有4条条带无对应的纯菌株，而纯菌株中有2株在混合菌群的图谱上没有相应的条带。

First,126 samples were gathered in the natural environment in which grew to the lactobacillus .

首先在各种适合于乳酸菌生长的自然环境中采集了126个样品，然后进行了乳酸菌的筛选试验，优选出2株L-乳酸产量较高且遗传性比较稳定的菌株，对该两株菌的种子培养条件及影响L-乳酸发酵的因素进行了系统的研究，同时对玉米芯L-乳酸发酵进行了初步的探讨。

Six overlapping DNA fragments of the Us region of infectious laryngotracheitis virus WG strain were amplified by PCR using primers based on the complete genomic sequence published in GenBank.

根据GenBank中的传染性喉气管炎病毒全序列(NCBI登录号：NC-006623)，利用Oligo6.2分析序列并设计6对引物，以ILTVWG株基因组DNA为模板，PCR扩增了长度为13.1kb的区域，得到了完整的WG株的Us区序列，初步鉴定了WG株的Us区基因结构。

Ten strains of adult specific pathogen free chickens are inoculated with Laryngotracheitis virulent virus,to in vestigate the clinical signs and the pathological changes by autopsy.Inoculate SPF embryonated eggs of the ten strains with virulent infections bronchitis virus and vaccine ND virus respectively.

用传染性喉气管炎强毒株感染10个家系150日龄BWELSPF鸡，观察比较临床症状和剖检所见病变；用传染性支气管炎强毒株和新城疫疫苗株分别接种这10个家系的鸡胚，收获胚液并测定其毒价。

The results showed that when the proportion of the resistant subpopulation within the h-VRSA was low, te level of the toxic factor and its pathogenicity to animals had no difference from that of the standard strain. If proportion of the resistant subpopulation increased the level of coagulase and lecithinase changing from positive from negative and the ring of hemolysis disappeared. The speed of proliferation of the organisms in the animal slowed down and the LD50 increased.

结果 当试验菌株中耐药亚群的比例较少时，其毒力因子及动物致病性检测结果与金葡菌标准菌株无显著差异；随着试验菌株中耐药亚群的比例增加，表现为血浆凝固酶、卵磷脂酶由阳性转为阴性，血琼脂平板上溶血环消失，细菌在动物体内增殖速度减慢，对动物的半数致死量(LD50)增加。

The recombinant RSV vaccinia virus VMS11G75F was constructed strictly according to the rules of biological products and all tests for contamination by bacteria, Chicken leucosis virus and mycoplasma gave negative results, so that vaccinia recombinant VMS11G75F could be a candidate of recombinant RSV live vaccine for human test.

HSV重组痘苗病毒VMS11G75F是严格按照人用疫苗株程序构建而成，并对多种外源因子污染(包括：细菌、鸡白血病病毒、支原体等)进行了检查，均为阴性，因此是一株可供人体实验观察的RSV基因工程活疫苗候选株。

Results Fifty-eight chronic liverish patients and 13 ASC persons were detected, in which HBV DNA levels with A83 mutations (log10) were (5.37±0.60),(4.02±0.51) copiesml^(-1) respectively. Ten blood donors were not found HBV A83 mutations.

结果 有HBV DNA前C区A83位点突变的58例慢性肝病患者血清中病毒变异株DNA水平为(5.37±0.60)copiesml^(-1)，13例无症状病毒携带者血清中病毒变异株DNA水平为(4.02±0.51)copiesml^(-1)，两者比较，t=7.54，P.001，差异有高度显著性；10例献血员血清中未检测到A83病毒变异株。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。