株

Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

Results In 585 staphylococcus, there were 406 staphylococcus aureus, which including 387 methicillin sensitive staphylococcus aureus and 19 methicillin resistant staphylococcus aureus. And there were 179 coagulase negative staphylococcus, including 20 methicillin sensitive coagulase negative staphylococcus and 159 methicillin resistant coagulase negative staphylococcus.

结果 585株葡萄球菌中，金黄色葡萄球菌406株，其中甲氧西林敏感的SA387株，甲氧西林耐药的SA19株；凝固酶阴性的葡萄球菌179株，其中甲氧西林敏感的CNS20株，甲氧西林耐药的CNS159株。

As showed above, we could draw the conclusion that H3 viruses could cause outbreaks or local epidemics in Shanghai for the immune of population to the new emerging antigenic drift was still unknown though there was strong immune barrier to the previous vaccine strain, H1 viruses also may caused epidemics as the population had a weak immune protection to the previous reference strains A/New Caledonia/20/99-like and part of the recent isolates were distingwishing from them and continued to evolute on the new reference strain, and H5N1 HPAI activities has expanded its geographical range thus increasing the size of the population at risk as the continuing emergence of human cases which give the virus an opportunity to evolve towards a fully transmissible pandemic strain. We should keep eyes on the human cases of avian influenza virus an its variation for the H5 isolates in Shanghai also has the possibility to infect human directly and the infection of H5, H9 viruses had previously occurred in the population especially in those contacting with animals.

综合上述分析结果，我们认为上海地区人群虽然对原有的H3亚型参考病毒株有较高的免疫保护，但H3亚型病毒HA已出现新的抗原漂移，人群现在对新变异株的保护水平尚未知，有可能在未来引起局限性暴发甚至地方性流行；人群对H1亚型流感病毒免疫保护水平低，同时H1亚型流感病毒株部分与原推荐疫苗株抗原性差别较大，并在现推荐疫苗株基础上继续变异，需警惕发生流行的可能性；H5N1亚型高致病性禽流感地域范围扩大，人间病例不断发生，存在演变成大流行株的威胁，上海市H5亚型禽流感病毒株存在跨宿主屏障感染人类的可能性，而且已发现人群尤其禽、畜类动物接触人群中存在H5、H9亚型流感病毒的既往感染，应警惕禽流感病毒的变异和人间病例的发生。

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株，没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA，作PCR检测。D1代获得5株阳性转化植株，分别是90035的4株，85—593的1株，转化率为3.8％。6、将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

Methods One hundred and fifty strains of Malassezia yeasts from pityriasis versicolor (29 strains), Malassezia folliculitis (16 strains), seborrheic dermatitis (49 strains), onychomycosis (33 strains), and healthy individuals(23 strains) were studied, according to physiological and morphological features in culture, and compared to standard strains.

方法以标准株作对照，用生理生化学及形态学方法将150株来源于花斑癣（29株）、马拉色菌毛囊炎（16株）、脂溢性皮炎（49株）、甲真菌病（33株）及正常人皮肤（23株）的马拉色菌进行分类及描述，并分析了各菌种在一些皮肤病的分布情况。

Results: Total extracted 75 bacterial trunks from the secretions of the nasal and sinus cavity when chronic nasosinusitis: 23 staphylococcus epidermidis trunks, 11 cor-ten steel staphylococci trunks, 8 flu hemophilus trunks, 7 pneumococcus trunks, and 17 other aerobes trunks and 9 anaerobe trunks.

结果：64例慢性鼻窦炎患者中鼻道、窦腔分泌物中共分离出75株细菌：表皮葡萄球菌23株，金黄色葡萄球菌11株，流感嗜血杆菌8株，肺炎球菌7株，其余各种需氧菌共17株，厌氧菌9株。

We extracted membrane protein of Mycoplasma hyopneumoniae MY-99 strain from its different passages strains MY-99l,MY-993 and rejuvenescence strain MY-992, then analyzed them by SDS-PAGE, the results show that the membrane protein of Mycoplasma hyopneumoniae MY-99 strain mutated during artificial culturing . According to the electrophoretic profiles, the membrane proteins of MY-991 include 7 protein bands: 94.4KD,57.5KD,46.0KD,36.0KD,32.4KD,20.4KD and 16.6KD.

采用0.4％TritonX-100结合KI提取猪肿炎支原体MY-99株不同代次的传代株MY-991、MY-993和复壮株MY-992的膜蛋白，并通过SDS-PAGE技术对其膜蛋白进行比较分析发现，在人工培养条件下猪肺炎支原体MY-99株的膜蛋白发生了一定的变异，其中MY-991株的膜蛋白有七条蛋白条带：94.4KD、57.5KD、46.0KD、36.0KD、32.4KD、20.4KD和16.6KD，MY-993株的膜蛋白有七条蛋白条带：94.4KD、57.5KD、46.0KD、41.0KD、36.0KD、32.4KD和19.5KD，MY-992株的膜蛋白只有两条蛋白条带：46.0KD和32.4KD。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

PART Ⅱ IMMUNOPATHOLOGICAL EVIDANCE OF SIALIC ACID STRUCTURE IN CAMPYLOBACTER JEJUNI AS THE CRITICAL ANTIGEN TO INDUCE PERIPHERAL NEUROPATHY To demonstrate the critical role of NANA structure in the pathogenesis of allergic peripheral neuropathy induced by Campylobacter jejuni and provide immunopathological evidence to confirm the supposition of molecular mimicry and cross-immunity between CJ LPS and gangliosides in nerve.

LPS免疫后第2周实验豚鼠的免疫血清中，抗LPS IgG抗体滴度均明显增高，第3周达高峰，第5周仍维持在峰水平，野生株和突变株LPS免疫血清中的抗-野生株LPS IgG抗体滴度较各自免疫前分别增高8倍和4倍，抗-突变株LPS IgG抗体滴度则较各自免疫前分别增高6.5倍和15倍；(2)全身免疫后第3周和第5周，野生株LPS免疫血清中检测到较免疫前增高6倍的抗-GM1 IgG抗体，而NANA缺失的突变株LPS免疫血清中一直未检测到抗-GM1 IgG抗体；(3)野生株LPS免疫组中有17.3％的坐骨神经原纤维发生以轴索变性为主（占65％）的免疫性损伤，与突变株LPS免疫组和对照组比较均有显著性差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。