株

Finally, 30 of actinomycosis of the initial screening and re-screening, to find the biological activity of strain, strain through 16SrDNA activity and biological characteristics of the determination of the detection, a preliminary to determine their taxonomic status.

最后通过对30株放线菌的初步筛选和再次筛选，找到有生物活性的菌株，通过对活性菌株的16SrDNA的测定和生物学特征的检测，初步确定其分类地位。

In a summary,in this study,the entire provirus genomes of EIAV L and DLA-EIAVprovirus were cloned and sequenced.Speculation about the function and structure of EIAVgenome was drawn upon the comparison of EIAV L,DA-EIAV,DLA-EIAV and other alienEIAV strains.

总之，本研究首次获得了我国DLA-EIAV和L株前病毒全基因克隆，确定了DLA-EIAV和L株前病毒基因组核苷酸全序列，并对EIAV L株、DA-EIAV、DLA-EIAV及国外相关的EIAV毒株进行了比较分析。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

The result came to departed in all in December 2006 in January 2004 Bowman does not move bacillus 194 individual plant, check goes out rate from 2004 2.4% rise to the 7.8%; section office 2006 to distributing in order to weigh disease ICU most (80 individual plant, 41.2%); and see more at patient of old age of 60 years old of above (95 individual plant, 49.0%);2006 year and mixed 2004 compared 2005, to 12 kinds of antibacterial content be able to bear or endure medical rate shows different level to increase, detached bacterium trunk of a tree is green to carbon mildew Xi kind sensitivity is highest.

结果2004年1月至2006年12月共分离鲍曼不动杆菌194株，检出率从2004年的2.4%上升至2006年的7.8%；科室分布以重症ICU最多(80株，41.2%)；且多见于60岁以上老年患者(95株，49.0%)；2006年度和2004年和2005年比较，对12种抗菌药物的耐药率呈不同程度增加，分离菌株对碳青霉烯类敏感性最高。

The strain L-3 that could produce raw starch-digesting glucoamylase were isolated from soil and mildewed rice. It was identified to be a Monascus Purpureus.

从土壤及霉变淀粉质材料等样品中分离获得到产糖化酶活性较高的菌株L-3，经鉴定为一种紫红曲霉；通过紫外线和硫酸二乙酯分别进行诱变，得到4株性能良好的糖化酶变异株，其中菌株7性能最好，命名为M.F7。

Results Five of the PCR amplifications were discovered and sequenced with 300 bp ...

结果对 5个阳性标本的 30 0bp部分M基因组片段序列分析表明均为SEO型汉坦病毒；毒株核苷酸序列比较表明：与其他SEO型毒株同源性均达 94%以上，而与其他HNT型毒株同源性均低于 72 %；毒株序列所构建的系统发生树显示，北京地区SEO型汉坦病毒可能至少存在 4个不同的进化分支和两个不同的汉坦病毒亚型。

And then re-use the nitroso muscle strain mutagenesis, in order to collect acid analog of GABA as a selection factor has been a 18% increase in production of new strains 42,, this new high-yield flask under the conditions of strain in the process were studied.

然后又对该菌株再采用亚硝基肌诱变，以撷氨酸结构类似物氨基丁酸作为筛选因子又得到一株新的产量提高18%的菌株42，，对此新的高产菌株在摇瓶条件下的工艺进行了研究。

At transcription level, the trap of ATCC 12228 and other biofilm positive clinic strains SE1457 and SE671 were very low compared to ATCC 35984, but the RNA Ⅲ was relative excessive in all strains, except in SE1457 △agr.

结果 比较分析形成生物膜的表皮葡萄球菌ATCC 35984株与不形成生物膜的ATCC 12228株生长中期的蛋白质组，发现ATCC 35984株的TRAP蛋白量明显高于ATCC 12228株。

The sequences analysis indicated that complete open read frame of F gene of 3 isolates was 1662bp and encoded F protein of SS3 amino acids/There were 6 identically potential asparagine-linked glycosylation sites and the deduced amino acid sequence of the cleavage site region of F protein of the 3 NDV isolates that had the same amino acids pattern as velogenic strain was ~(112)R-R-Q-K/R-R-F~(117), which was accordant with the results of mean death time of chicken embryos and intracerebral pathogenicity index flCPI.

序列分析结果表明，所获得的3株分离株F基因完整的开放阅读框长度为1662bp，共编码F蛋白的553个氨基酸：QH3、HU和A4株均有相同的6个潜在的糖基化位点，其F基因的裂解位点为~(112)R-R-Q-K／R-R-F~(117)，符合强毒株裂解区氨基酸组成的特征，且与MDT和ICPI的测定结果相吻合。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。