株

The age class structure of Salix oritrepha population in alpine, cloudy and wet area of east Qilian Mountian is studied ,on two levels of genets and their ramets and sprouting branches .The results show that genets population is very short of the first and second class seedlings,and most of seedlings belong to the third and forth classes, and the development trend of population on this level is stable;on the other hand,the age structure of remets and sprouting branches population is more complete,and its age class distribution chart likes triangle, belonging to increasing type.In general,Salix oritrepha population in this area is increasing, but its reproduct way have changed in community succession process in order to accommodate varying circumstances.

从无性基株个体种群和无性克隆分株及萌生枝种群两个层次上对东祁连山高寒阴湿地区山生柳种群年龄结构进行分析，结果表明：无性基株个体种群 0~10 龄级幼苗贮备严重不足，11~20 龄级苗木所占比重最大，种群动态呈稳定型；无性克隆分株和萌生枝种群年龄结构比较完整，结构分布图呈正三角形，种群动态表现为增长型；总体上来讲山生柳种群呈增长趋势，只是在演替过程中为适应环境其繁殖方式有所改变，导致现阶段山生柳种群两个层次结构状态。

Pestis pathogenicity. Methods: We utilized "whole genome shotgun" approach to get the genome sequence of 91001. Based on the finished and annotated genome sequence of 91001, as well as previously published genome sequence of C092 and KIM, we performed detailed comparative genomics analysis on their chromosome and plasmids. For the important phenotypes - nitrate reduction and glycerol fermentation, we carried out large scale screening among 257 strains isolated in China to clarify the genetic mechanism of these two phenotypes.

采用全基因组鸟枪法测定91001菌株的全基因组序列，利用比较基因组方法对91001与另两株鼠疫耶尔森氏菌(CO92和KIM)的全基因组进行比较研究；同时针对一些有意义的基因在多株鼠疫耶尔森氏菌的中国分离株中进行了验证；并在基因组研究的基础上开展了初步的蛋白质学研究。

Four strains which could effectively degrade dye were separated from bark with brown or ivory white locus ferrugineus.One strain with the strongest MnP activity was selected by measuring enzyme activity with natrium lacticum,which is

本研究从长有褐色或者乳白色锈斑的树皮上分离出4株可以降解染料的菌株，结合乳酸钠法测酶活，筛选出1株产酶能力较高的菌株W3，其MnP活力为8.59Wg，经形态鉴定为黄孢原毛平革菌。

CG strains were isolated from the mango fruits with anthracnose symptoms in different locations on Guangdong and Hainan Province, China. The pathogenicity and sporulation capability of these isolates were compared, and the isolate LCG5 from CATAS, Danzhou City, Hainan Province, China were Chosen for further use in this study for its high virulence on mango fruits and also good sporulation capability.

分别从海口、湛江、两院三地采集到CG菌株进行纯化培养，通过芒果接种实验，对发病率、发病指数及菌株的产孢能力三项测试，最终确定选用接种发病率为100％，病情指数为71.1，菌株产孢能力达1.2×10~6个／ml的两院第5号菌株(缩写为LCG5)，其致病力比较强，产孢能力可以保证了转化体系的成功建立，方便对突变体库的致病和产孢相关突变体的筛选。

Three standard and two isolated strains of E.coli were used for drug sensitivity test on Chloramphenicol, Ciprofloxacin Lactate, Rifampicin, sulfamonomethoxine, Florfenicol, Ceftiofur Sodium as well as the antimicrobial synergists sulbactam sodium and TMP. The bacteriostasis results of the antibiotics against E.coli In vitro were compared when they were used separately or combined with the synergist.

试验用3株大肠杆菌标准株和2株地方分离株，选择氯霉素、乳酸环丙沙星、利福平、磺胺-6-甲氧嘧啶钠、氟苯尼考、头孢噻呋和抗菌增效剂舒巴坦钠、TMP进行药敏试验，比较几种药物单一或与增效剂合用对大肠杆菌的体外抑菌效果。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Lentinula edodesPegler, the shiitake mushroom, is one of the most widely cultivated mushrooms worldwide. The purpose of the project is to investigate the characteristics of 48 isolates or cultivated strains and 10 foreign countries strains.

香菇是广为世界栽培的主要食药用菌之一，亦为台湾主要栽培的食药用菌，本研究收集目前台湾各地主要栽培香菇菌株48株及外国菌株10株。

The average rate of volume shrinkage of oven-dried in Larix gmelini was 6.34%, the average rate of radial shrinkage of oven-dried in Larix gmelini was 1.98%, the average rate of tangential shrinkage of oven-dried in Larix gmelini was 4.23%. Variance of volume shrinkage of oven-dried in Larix gmelini was more significant among trees, and its value ranged from 4.48% to 7.59%. Variance of radical shrinkage of oven-dried in Larix gmelini was not obviously among trees, and its value ranged from 1.27% to 2.69%. Variance of tangential shrinkage of oven-dried in Larix gmelini was more obviously among trees, and its ranged from 2.73% to 5.00%. Difference of value of / among trees was obvious, and its value ranged from 2.15% to 3.58%.

兴安落叶松木材全干体积干缩率的平均值为6.34%，全干径向干缩率的平均值为1.98%，全干弦向干缩率的平均值为4.23%；全干体积干缩率的株间差异较显著，变异范围为4.48%-7.59%，全干径向干缩率株间差异不显著，变异范围为1.27%-2.69%，全干弦向干缩率株间差异较显著，变异范围为2.73%-5.00%；全干弦向干缩率与全干径向干缩率的比值株间差异较显著，变异范围为

The bacterium was characterized by routine bacteriology and apxⅣ-PCR with purified bacteria, which included 4 strains serotype 4, 1 strain serotype 3 and 1 strain no serotyping by GDT.

结果从患病猪的肺、关节分离到胸膜肺炎放线杆菌6株，其中，4株4型，1株3型，1株未定型。

Methods The serotyping, hybridization and RAPD typing methods were used. Seven strains of GBS were detected in 2 pairs of mother-baby, which included 2 strains from the 2 mothers and 5 strains from their babies with early and late-onset infections. Eighty-eight strains of GBS were detected in vaginas of pregnant womens.

采用血清学、核酸杂交和PARD方法对从2例诊断为早发型GBS肺炎和晚发型GBS脑膜炎患儿体内分离的5株GBS和其母阴道分离的2株GBS菌株作母婴配对分析，对88株孕妇生殖道携带GBS进行RAPD分型研究。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。