株

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列，运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析，根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增，检测沙门氏菌和大肠杆菌Ⅰ类整合子；扩增出了相应基因片段并测序；61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子，Ⅰ类整合子的检测率分别为：81.97％和66.67％。

A fungus CTB430-1 with resistance to copper and zinc was isolated from soil at a copper and Zinc Tailing of Linxiang in Yueyang city, Hunan province. The fungus was identified by analyzing the morphology and measuring the ITS sequence. The effects of pH, initial concentration, co-ions on the growth of CTB430-1 and bioaccumulation of Cu2+ and Zn2+ were studied. The protein and glutathione in the cells were determined by using the coomassie brilliant blue method and iodometric method, respectively.

摘 要：从湖南省岳阳市临湘铜锌尾砂坝土壤内分离、纯化得到1株抗铜和锌的菌株CTB430-1，分析该菌株形态和测定菌株ITS基因序列鉴定菌种，研究不同pH值、金属离子初始浓度和金属离子共存对菌体生长量和富集重金属的影响；分别采用考马斯亮兰法和碘量法测定菌体内可溶性蛋白和还原性谷胱甘肽含量。

Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起流行的稻叶1d为同 RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

Different from the importation characteristic before 90s, strains after 90s were highly isogenous, suggested that the cholera epidemics in Sichuan province after 90s were endemic source. Inaba 1d strains from national wide epidemic in 2001 and 2002 were the same RT. Strains typed as different phagetypes (phagetype 6, phagetype 13 and phagetype 1) might be from a same clone.

除82-007、95-001外，所试菌株均具有致病性；20世纪90年代以前四川省发生的霍乱为输入性，90年代后四川省流行的菌株高度同源，具有地方疫源性；2001、2002年在四川省乃至全国引起大流行的稻叶1d为同一RT型，为传入性霍乱；噬菌体分型6型、13型菌株和1型菌株可能来自同一克隆系。

Results］ Pass to solve the curvilinear analys is to the liquate linear , examine the 2 examples :YMDD variation stub in earlier in liver in B that had not yet any treatment sufferer, occupy 6.67%;Pulling the rice man settle the sufferer who cure to examine the 13 examples :YMDD variation stub in a year, occupying 26.00%;Pulling the rice man settles two years in treatment to examine the 25 examples :YMDD variation stub in the sufferer, occupying 50.00%, pulling the rice man settle to examine the 9 examples :YMDD variation stub with interferon in the sufferer of uniting the treatment, occupying 45.00%.

结果］通过对熔解曲线的分析，在未经任何治疗的乙肝初发患者中检出YMDD变异株2例，占6.67%；拉米夫定治疗1年的患者中检出YMDD变异株13例，占26.00%；拉米夫定治疗两年患者中检出YMDD变异株25例，占50.00%；拉米夫定和干扰素联合治疗的患者中检出YMDD变异株9例，占45.00%。

Analysis and fitting of growth curves in Liyang chicken;2. The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra,3 strains isolated from manganic nutrient agar plate had advantage on growth.

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌，进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线，其中NAC平板上筛选的4株拮抗菌的抗菌范围较广，锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

In order to discover acid-tolerant Rhizobium meliloti that can nodulate alfalfa high-effectively on acidic soil, researches were explored to isolate rhizobia from alfalfa nodules on acidic soil (pH5.2), methods based on the thesis of adaptive acid-tolerance response of the Rhizobia were adopted, six strains were isolated, which could grow on solid medium with pH4.8 steadily. In addition, the nodulation characteristics, growth curves and mean generation time under different acidity of the strains were examined.

为获得能够使紫花苜蓿在酸性土壤中高效结瘤的耐酸根瘤菌，该研究从酸性土（pH5.2）上生长的紫花苜蓿根瘤中分离到若干菌株，利用根瘤菌对酸的适应性耐受反应筛选到六株能够在pH4.8的固体培养基上稳定生长的菌株，并检测了这六株菌的结瘤特征、不同酸度下的生长曲线和平均代时。

The new plant type Indica and Japonica varieties showed somewhat check heterosis whencrossed with one of the four sterile lines. America Japonica varieties showed few fillers andlower seed set ratio and Africa Japonica varieties, Nuda varieties, Javanica varieties have fewtillers and taller plant height, so they could not be used directly in Japonica hybrid ricebreeding program.

美国稻的分蘖少且结实率较低，产量优势不大；非洲稻的分蘖少，实粒数也少，株高太高，无法直接利用；新株型粳稻与培矮64s、N422s配组有一定的优势，新株型籼稻与N422s、108s配组的优势也较大，在华北两系杂交粳稻育种中也有一定的利用价值；光壳稻和爪哇稻的株高偏高，无法直接利用，可间接利用这两个生态型千粒重大的特点。

Through DOT-blot hybridiztion the result indicated SA215 and SA215 were of the same distribution on animal ,simply planting the following trigeminal ganglion .21-day-old SPF pigs inoculated intramuscularly SA215 strain could be protected from being affected at day 28 after oronasal challenge-exposure with 106 PF.U high-dose virulent PRV Fa strain and at day 42 after i.m.

SAZ巧株lml接种21日龄健康仔猪(伪狂犬病和猪瘟检测阴性)，在接种后28天用10毕FU的PRV Fa强毒株进行滴鼻攻毒，42天用猪瘟强毒SM株血毒lml肌肉注射攻击，结果表明接种猪能抵御两次病毒攻击，而同条件下对照结果成立，表明SAZ 15株具有良好免疫原性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。