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Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Results The recovery rate of three representative bacteria determined by the culture media dilution method, were more than 70% and the recovery rate of two representative mold and fungus determined by the membrane filtration methods with 1000 ml washed, were more than 70%.

结果 采用平皿法中的培养基稀释法测定样品人工污染3株细菌代表菌株的菌回收率均于70%，采用薄膜过滤法在冲洗1 000ml时，测定样品人工污染2株真菌代表菌株的菌回收率均大于70%。

Strains of fungi were detected, among them, 62 (71.3%) were Monilia, 17 (19.5%) mold fungus, and 7 (8.0%) budding fungus. The most common Monilia were Monilia albicans, slick Monilia and Oidium tropicale.

感染菌谱中真菌87株，其中念珠菌62株(71.3%)，毛霉菌17株(19.5%)，酵母菌7株(8.0%)，念珠菌中常见的菌种依次为白色念珠菌、光滑念珠菌和热带念珠菌。

Methods Genomic DNA was extracted from 50 strains of S.schenckii, including 49 clinical isolates and 1 referrence strain, as well as from 1 strain of Mucor, Aspergillus fumigatus and Candida albicans, respectively, as controls. Four primers were designed according to previous studies, and screened by PCR.

以50株不同地区来源的孢子丝菌临床分离株及标准株的基因组DNA作为研究样本，以毛霉、烟曲霉、念珠菌临床分离菌株基因组DNA作为对照，通过特异引物PCR扩增的方法筛选国内外文献中报道的4对孢子丝菌引物。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

It also indicated that the oospore production of Mtr isolates was obviously less than those of the parental isolates, and that the mycelium production of Mtr isolates was a little reduced compared to those of the parental isolates in liquid culture.

研究结果还表明，抗性菌株的卵孢子的产生量也明显低于其亲本菌株，抗性菌株在液体培养基中的菌丝较其亲本菌株生长量有所减少。

12 Strains possessing higher peptidase activity were isolated from Chinese traditional fermented food.

试验从中国传统发酵食品中分离出12株具有较高肽酶活力的菌株，经进一步筛选，得到了两株具有较好促进干酪成熟能力的菌株，有望作为附属发酵剂应用于切达干酪生产。

In order to evaluate the capability of lignin degradation and pitch elimination from Masson pine chips by nature white-rot fungi, 20 white-rot fungus strains were obtained by petri dishes screening and petri dishes color-reaction screening. One G strain with the lowest Mycel/Col value was then inoculated onto the surface of Masson pine chip, and the degradation rates of lignin and pitch were measured after 3, 5, 7, 9 days, respectively.

为评价从野外筛选得到的白腐菌对造纸原料马尾松的木素降解和树脂脱除能力，将带菌样本经室内平板初筛及平板变色反应复筛，得到了20株纯白腐菌菌株，并对变色系数最小的一株菌株G进行松木片固体接种试验，测定3、5、7、9天后松木片的木素、树脂降解情况。

In order to evaluate the capability of lignin degradation and pitch elimination from Masson pine chips by nature white-rot fungi,20 white-rot fungus strains were obtained by petri dishes screening and petri dishes color-reaction screening.

为评价从野外筛选得到的白腐菌对造纸原料马尾松的木素降解和树脂脱除能力，将带菌样本经室内平板初筛及平板变色反应复筛，得到了20株纯白腐菌菌株，并对变色系数最小的一株菌株G进行松木片固体接种试验，测定3、5、7、9天后松木片的木素、树脂降解情况。

A tree whose hungry mouth is prest againsr the earth's sweet flowing breast; a tree that looks at god all day, and lifts her leafy arms to pry; a tree that may in summer wear a nest of robins in her hair; upon whose bosom snow has lain; who intimately lives with rain.

一株树，他的饥渴的嘴吮吸着大地的甘露。一株树，他整日望着天高擎着叶臂，祈祷无语。一株树，夏天在他的发间会有知更鸟砌巢居住。一株树，白雪躺在他胸上，他和雨是亲密的伴侣。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。