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PART ONEInvestigation on Hp CagA+ strain infection condition and pathogenesis risk analyze in patients of carotid atherosclerosisObjective To observate pathological changes of carotid atherosclerosis as well as Hp infection situation, examinate blood fats, blood serum hs-CRP, blood plasma YKL-40, blood serum sCD14, analyze relations between Hp or Hp CagA+ strain and carotid atherosclerosis, and explore possible mechanism of carotid atherosclerosis caused by infection.

然后进行基础动物实验，应用Hp CagA+菌株干预高脂血症大兔，建立Hp CagA+菌株感染致兔颈动脉粥样硬化模型，进行血清Hp抗体、hs-CRP、血浆YKL-40水平检测、斑块内Hp DNA，进一步求证Hp CagA+菌株感染和颈动脉粥样硬化形成的相关性，Hp CagA+菌株感染是否可促进颈动脉粥样硬化的形成，深入探讨其可能机制，以期为颈动脉粥样硬化形成的病因学研究提供实验基础，为临床治疗颈动脉粥样硬化提供新的策略，从而完善其防治模式。

Characteristics of engineering S.aureofaciens growth and metabolism were studied in 〓fermenter.

在溶解氧充足的条件下（≥30%的空气饱和度），透明颤菌血红蛋白表达，对金色链霉菌生长代谢未产生明显影响，工程菌株与参比菌株的生长代谢特性基本一致，工程菌株和参比菌株金霉素最终浓度分别为22905u/mL、22896u/mL。

The plan area is 20,000 Chinese acres, in the area millennium baiera 3, 200 year above gingko tree 26000, hundred year above gingko tree 18500, Bears fruit gingko more than 100,000, Was continuous a kilometer gingko tree to compose the unique gingko natural forest style, Just likes the canopy converges, four seasons scenery each has his good points: Spring the layer illuminates green, A piece of light green; If the summer is green and luxuriant, the deep shade obstructs the date; The golden fruit is autumn countless, the full garden smells as sweet; A cold winter arrogant day, welcomes the wind scoop snow, pleasant, completely relaxed, Caused that America and Africa, the European and American 16 country's experts and the regional tourists stream in the sightseeing inspection, the concerned experts reputation is "miracle of the nature, paradise of the leisure".

规划面积为2万亩，区内千年古银杏3株，200年以上的银杏树26000株，百年以上的银杏树18500株，挂果银杏10万多株，绵延上千米的银杏树组成了独特的银杏自然森林风貌，犹如华盖云集，四季景色各有千秋：春季嫩枝照绿，一片葱绿；如夏郁郁葱葱，浓荫遮日；秋天金果累累，满园飘香；寒冬枝傲天，迎风斗雪，令人赏心悦目，心旷神怡，引得亚、非、欧美16个国家的专家学者和各地游客纷至沓来观光考察，有关专家学者誉之为"自然之奇迹，休闲之胜地"。

In this study,we focus on the interaction between nuclear modifier gene MTO2 and aminoglycosides,and have received the following innovations.1,We here report first that the knockout of MTO2 significantly inhibited mtDNA p_(454)~R mutant's sensitivity to aminoglycoside antibiotics.Here are the results:(1)In neomycin GYP medium,when mitochondria genes were wild type,whether MTO2 knockout or not,the growth of yeast strains has no significant difference;when mitochondria genes were complete deletion,whether MTO2 knockout or not,the growth of yeast strains also has no significant difference;Interesting,when mtDNA P_(454)~R,wild-type MTO2 strains were hardly survival in neomycin GYP medium,but the growth of MTO2 knockout strains were only partly inhibited,there was a significant difference performance of neomycin sensitivity between them.

具体结果如下：(1)在含有新霉素的葡萄糖培养基上，mtDNA为野生型、MTO2敲除与否，相应的酵母菌株的生长没有显著差异；mtDNA完全缺失、MTO2敲除与否，相应的酵母菌株的生长也没有显著差异；然而，当mtDNA存在P_(454)~R突变时，MTO2野生型菌株几乎完全不能生长，而MTO2敲除菌株的生长只受到部分抑制，表现二者对新霉素的敏感性存在显著差异。

The phylogenetic trees revealed the GD3 strain was a intergrade virus strain evoluated from classical PRRV CH-1a strain to the PRRSV JXA1 and HUN4 mutant strains indicated by 97.1 %, and 97.2 % nucleotide homology with JXA1 and HuN4 strain, 94.9% nucleotide homology with CH-1a strain.

序列分析结果表明，GD3株在分子遗传演化上介于PRRSV变异毒株(HuN4 株、JXA1株)和CH-1a之间，与HuN4和JXA1之间的相似性为97.1 %和97.2 %；与CH-1a的核苷酸相似性为94.9 %。

Three mold stains, C122803、D081506 and D081513, which had higher ability of producing inulase were obtained by using transparent circle method as initial screening and rocker method as rescreening.

通过透明圈法初筛及摇瓶复筛，获得产菊粉酶能力较高的霉菌菌株3株，为C122803、D081506和D081513，这3株菌株的酶活分别达到：C122803：1.411 U/ml；D081506 ：1.895U/ml；D081513 ：1.792U/ml。

The thymidine kinase gene of infectious laryngotracheitis virus Beijing E2 strain was amplified by PCR, cloned into pGEM-T vector, and sequenced. Sequence analyses showed that the TK gene was very closed among sequenced ILTV strains.

以纯化的传染性喉气管炎病毒北京E2株基因组DNA为模板，通过PCR方法扩增了ILTV北京E2株的完整TK基因，将其克隆到pGEM-T载体上，并测定了序列，与其它ILTV毒株TK基因比较说明，ILTV不同毒株的TK基因高度保守。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

Leishmania promastigotes antigens have good stability and heat-resistant nature. The sensitivity of 901 and 801 antigen were better, so we thought it could be used for serologic epidemiology investigation in XinJiang.

利什曼原虫前鞭毛体抗原有较好的稳定性，耐热性极好；901株、801株抗原的敏感性、特异性较好，可做为新疆血清流行病学调查所用的抗原株；801株的特异性更好，可用于黑热病的特异性抗体的检测及研究。

The morphological analysis of strain EMZY-1 showed that it was a straight rod, about 0.3×1.7 in size, and spherical and terminal spore appeared. Results of physiological and biochemical test showed that strain EMZY-1 was a Gram-positive bacteria, and could not grow when temperature was lower than 10℃ or higher than 60℃, pH was lower than 5.0 or higher than 12.0, and the concentration of NaCl was more than 10%. The strain EMZY could grow on the medium as a of sugar, glucose and mannite. The strain could use saccharose, glucose and mannitol as carbon resource, and NH4(superscript +) and NO3(superscript -) as nitrogen resource.

通过对该菌株的形态、培养特征、生理生化特征的研究以及16S rDNA序列分析表明：该菌株为革兰氏阳性菌；细胞为棒杆状，端生孢子，大小约0.3×1.7；温度低于10℃、高于60℃无明显生长；pH低于5.0、高于12.0无明显生长；NaCl质量浓度达到10%菌株不能生长；能在蔗糖、葡萄糖、甘露醇为C源的培养基上生长；NH4、NO3为菌株良好N源。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。