株

The results were as followed: 1 Mutagenesis Breeding of High Tannase Activity Strains and preparing of Tannase As a original strain, Asp. niger CY was mutated by UV treatment. One strain was obtained from the mutants.

全文实验结果如下： 1 单宁酶菌种的选育和单宁酶的制备以一株产单宁酶的黑曲霉为出发菌株，进行了菌株的诱变选育，得到了一株单宁酶高产菌株。

Hemagglutinin thermostability and hemagglutination spectrum tests: Large variations in hemagglutinin thermostability of 29 SIV isolates from various provinces were detected, with 22 being thermolabile, 2 moderately thermostabile and 5 thermstabile. Two human Influenza Virus strains were either thermal stable or non-stable.

血凝素热稳定性和血凝谱测定结果证实，不同地区SIV分离株的血凝素热稳定性差异较大；22株表现为热不稳定性，2株为中等热稳定型，5株为热稳定型；2株人流感病毒分别表现为热不稳定型和热稳定型。

Of 549 isolates, 20 isolates (about 3.6% of the total isolates) showed significant activity against P. ultimum and 15 isolates (about 2.8% of the total isolates) against R.

结果表明，对终极腐霉、立枯丝核菌的半抑制稀释倍数（ID50）在10以上的活性菌株分别为20株（占总菌株的3.6%）和15株（占总菌株的2.8%）。

In this study, the author used a molecular marker SCW-37 that was specific for BYDV resistant gene Bdv2, and two molecular markers that were specific for powdery mildew resistant genes Pm13 and Pm21 to analyze 54 candidate plants. As a result, 1 plant pyramided Pm13+Pm21+ Bdv2 resistant genes, 1 plant pyramided Pm13+ Bdv2, 11 plants pyramided Pm21+ Bdvi, were selected for further breeding practice.

本研究利用黄矮病抗性基因Bdv2特异的SCAR标记SCW-37，及对白粉病抗性基因Pm13和Pm21特异的标记，对54株杂交回交后代进行扩增，检测选择到Pm13+Pm21+Bdv_2抗病优质材料1株1484-16；Pm13+Bdv_2一株1484-9；Pm21+Bdv_2基因聚合株11株。

Results We isolated 2 strains of Bdellovibrio sp.(5#-12 and 5#-sh06) from sea mud of Shenzhen bay. Both strains grew between 20℃ and 35℃, with 25℃ and 30℃ as optimal temperature for 5#-12 and 5#-sh06, respectively. They grew between pH 6.1 and 8.6, and the opti℃mal pH for both was 7.2. Lysis experiments on 58 strains of pathogens were conducted and the results showed that 5#-12 and 5#-sh06 lysed 46 and 48 strains, corresponding to 79.3% and 82.8% of lysis abilities. Taken both two Bdellovibrio strains together, they lysed 96.6%(56 strains) of tested pathogens and 100% of tested vibrios (39 strains).

从深圳湾海泥中分离出2株蛭弧菌，分别命名为5#-12和5#-sh06，它们可在20℃~35℃范围内生长，最适温度分别是25℃和30℃；生长pH范围6.1~8.6，最适pH均为7.2；2株蛭弧菌可分别裂解46和48株试验菌，各占总试验菌株数（58）的79.3%和82.8%；联合2株蛭弧菌，可裂解56株试验菌，占总试验菌株数的96.6%；同时，它们一起能将所有试验弧菌裂解。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

The microorganisms was domesticated by adding a certain amount of lead in the soil, and three lead-tolerant strains including Pb-R-1, Pb-R-2 and Pb-H-3 accordingly originating from 300 mg/L, 750 mg/L and 900 mg/L lead medium were isolated with a series of PDA medium of different lead concentrations. Through the identification, Pb-R-2 was confirmed as Aspergillus sp., Pb-R-3 as Penicillium sp. The trials of transferring culture showed that all strains had the stable heredity of lead tolerance in different composition PDA medium.

通过向土壤中添加一定量的铅来驯化土壤微生物，然后利用一系列不同铅浓度的PDA培养基分离耐铅微生物，结果获得一株耐300 mg/L铅的细菌Pb-R-1和二株分别耐750 mg/L和900 mg/L铅的真菌Pb-R-2和Pb-R-3，通过鉴定得知，Pb-R-2菌株为曲霉，Pb-R-3菌株为青霉；不同成分培养基传代培养实验表明，3个菌株有稳定的耐铅遗传性；通过液体悬浮培养获得：Pb-R-1、Pb-R-2和Pb-R-3对Pb(上标 2+)的吸附率依次为35.5%、75.5%和48.2%。

During the first cross, 4 different white monokaryotic strains were hybridized with 14 kinds of yellow monokaryotic ones to obtain light-yellow strains, which were then used to fruiting experiment. In the second cross, five kinds of yellow monokaryotic strains, which were observed monokaryotic fruit body, and 12 types of white monokaryotic ones were crossed to acquire hybrid strains, which were also to the experiment of fruit body formation.

第一轮杂交，运用4个不同的白色单核菌株和14个不同黄色单核杂交，获得系列浅黄色杂合子菌株，用于出菇实验；第二轮杂交，运用5个能够获得单核子实体的不同黄色单核菌株与12个不同的白色单核菌株相互杂交获得杂合子菌株，用于出菇实验。

In this thesis, eight cold-adapted producing bacterium strains were screened from the mud of Parece Vela basin in Pacific Ocean of 5010m deep.

本论文通过琼脂平板透明圈法从太平洋帕里西维拉海盆5010m深的底泥中筛选出八株具备低温脂肪酶活力的菌株，其中的两株生理生化特征相近的菌株D1、D2上清中的酶活力最高，其它菌株所产的酶很大一部分粘附在细胞壁上，上清中的酶活力很小。

The purpose of the research was to obtain bacterial polyhydroxyalkanoic acids producers with good characters. With bottom sludge from Donghu Lake, bum soil from hillside, fertile vegetable soil and oil-soaked soil as initial samples, the bacterial strains with ability of synthesizing PHAs were screened not through enriching, isolation and purification, staining with Sudan black B and qualitative and quantitative analyses by infrared spectroscopy and gas chromatography. The results of staining with Sudan black B showed that there were 3 strains with ability of synthesizing PHAs among the 12 isolated strains and their numbers were Zj-5, Zj-8 and Zj-9 resp. All the 3 strains took on adsorption peaks at the points near 1741 cm^(-1) and the infrared spectrums of the strains Zj-8 and Zj-9 were similar.

［目的］获取性状优良的聚羟基烷酸合成菌［方法］以东湖底混、山坡裸地土、肥沃菜园土和油脂浸润土为初始样品，经富集、分离纯化、苏丹黑B染色、红外光谱及气相色谱定性定量分析筛选出具PHAs合成能力的细菌菌株［结果］苏丹黑B染色结果表明，在分离出的12株细菌中有3株细菌具有PHAs合成能力，其编号分别是Zj-5、Zj-8和Zj-9.3株细菌均在174cm^(-1)附近呈现吸收峰，菌株Zj-8和Zj-9的红外谱图相似。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。