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To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

Results:67(30.45%)strains of H.pylori were isolated from gastric mucosa;The positive of H.pylori rate was 20.15%(27/134),48.72%(38/78)and 25.00%(2/8)in gastritis,gastric ulcer and gastric cancer respectively.Among 67 isolates,23 strains 34.33%(23/67), were resistant to clarithromycin,and other stains were sensitive;No intermedial sensitive strain was found in these isolates.

结果：220例胃粘膜标本H.pylori培养阳性率为30.45%(67/220)；胃炎培养阳性率为20.15%(27/134)；消化性溃疡培养阳性率为48.72%(38/78)；胃癌培养阳性率为25.00%(2/8)；消化性溃疡组H.pylori培养阳性率明显高于胃炎组和胃癌组。67例H.pylori菌株克拉霉素耐药23株，占34.33%(23/67)；敏感株44株，占65.67%(44/67)；未发现克拉霉素中介株。

Two strains that are resistant to nisin with high levels (up to 2000IU/ml in MRS broth ) were screened from 362 strains. One strain Ls-5105 isolated from pickles was systematically identified as leuconostoc mesenteroides subsp. mesenteroides on the basis of morphology, physiological and biochemical features with modern analytical methods and classical methods.

经过分离筛选，从362株菌中得到两株能够耐受2000IU／ml乳链菌肽的高抗菌株，利用现代分析技术与经典分类法对其中一株菌Ls-5105进行系统细菌学鉴定，鉴定该菌株为明串珠菌属肠膜明串珠菌肠膜亚种Leuconostoc mesenteroides subsp。

T standard strains and one fermentative strain MZ1 as templates, PCR results showed that 16 strains, which may be lysogenic strains, produced the product with the size about 750bp as anticipated.

t标准菌株和生产菌株MZ1（血清型H3a3h）的过夜培养物作模板，结果有16株菌株包括MZ1能扩增出大小约为750bp的预期产物，推测这16株菌为溶原菌。

Six bacteria and five mildews have been separated from 119 strains, wliich grew well on the culture media contained lignin. The result of taxonomic identification is that XI, X2 and X3 belong to Pseudomonas, X4 and X5 belong to Xanihomonas, X6 belongs to Mycoplana. A preliminary study has been carried out on the capacity of lignin degradation of the 6strains of bacteria, and strains X2 and X4 were chose to carry out the experiments. The most favorable temperature for the 2 strains is 30癈, and the pH is 7; After being treated by ultraviolet rays, activity of all the three kinds of lignin-degrading enzyme produced by the strains can be increased greatly, the bacteria increasing rate is remarkably improved.

从119个菌株中分离筛选出了6株细菌和5株霉菌，它们可以在含有木质素的培养基上良好生长：细菌分类学鉴定结果为X1、X2和X3：假单胞菌属，X4和X5：黄单胞杆菌属，X6：枝动杆菌属；对6株细菌降解木质素性能进行了初步的研究，选定X2和X4为实验用菌种，两株菌适宜的生长温度为30℃，最适初始pH值为7；经紫外线照射诱变法处理，可使它们产生的三种木素分解酶活性均有明显的提高，细菌增长速率比诱变前明显提高。

Strains, which were white or light brown when grew on the lead acetate media plates, were selected. Then, the different differential medium plates containing NaiS and as the only sulfur sources were used to select 24 strains, which grew well on plates, but didn't grow well on Na2SO3 plates.

选择在醋酸铅显色平板上菌落颜色为白色或浅棕色的菌株(H_2S产生少的菌株)111株，分别对应点接于以Na_2S和Na_2SO_3为唯一硫源的两种鉴别培养基上，选择在Na_2S培养基上菌落较大，同时在Na_2SO_3培养基上菌落较小或不生长的突变菌株24株。

Phylogenic tree of SHZH03, SHZH98 and some other Enterovirus71 based on the genome sequences was analyzed.

目的 对SHZH03株、SHZH98株、亚洲流行株(台湾98年、日本99年、及新加坡2000和2001年流行株等)以及一部分欧洲流行株等的结构蛋白基因进行遗传进化分析。

Now it preserves more than 1750 domestic and international bacterial microorganism, including more than 380 bacteria, more than 750 saccharomycete, more than 620 chitomycetes, roughly cover all kinds of bacterial microorganism in this field, among them some good microorganism's production reach the international high level.

目前该中心保藏国内外各类工业微生物菌种1750余株，包括细菌380余株，酵母菌750余株，丝状真菌620余株，基本覆盖了食品与发酵行业各类生产和科研用微生物，其中许多优良菌株的生产性能具有较高水平。

It was showed that the growth speed of hypha, sclerotinia production and the pathogenicity of strains had little relation with host and geographical location. The ability of oxalic acid production of strains had positive relation with the pathogenicity. In order to study the nutrition aptness, 8 kinds of culture mediums were tested and the result showed that S.

sclerotinia不同菌株菌丝的生长速度、菌核产量、致病力与其地理来源和寄主来源关系不大，对12个代表菌株的草酸产生能力的测定表明，菌株的草酸产生能力和病菌的致病性基本呈正相关，致病力较强的菌株草酸产生能力较强；采用8种培养基对代表菌株营养条件进行了研究，结果表明核盘菌S。

The results showed that the sex ratio of trees was 1 m 2 f, and the natural T. cuspidata tree was able to sexually reproduce for many years. Male trees had fecundity when the diameter at breast height ranged from 1.5 cm to 92 cm, while female trees had fecundity when the DBH from 9.5 cm to 68.1 cm. Male trees typically flowered at the age about 20 years earlier than female trees. The numbers of microstrobili were 3 times more than that of megastrobili, and only 1/10 megastrobili developed into seed. The numbers of strobili and seed were correlated with DBH, crown diameter and height of trees; however, no significant correlations were observed with other site factors. Numbers of strobili decreased from the upper to lower canopy layers, while the quantity of seed decreased from the middle, upper to lower canopy layers. Seed-setting rate increased from the upper to lower canopy layers. Numbers of strobili and seeds in different directions of the canopy were not uniform, but the differences weren't significant.

结果表明：现实东北红豆杉天然种群中，♀♂性比为1：2；天然东北红豆杉有性生殖持续时间长，雄株胸径在1.5~92cm，雌株胸径在9.5~68.1cm保持生殖状态，雄株较雌株早约20年进入花期；天然东北红豆杉小孢子叶球数量平均为大孢子叶球数量的3倍，大孢子叶球转化为果实的数量不足1/10，孢子叶球数量、结实数量与样株的胸径、冠幅、树高显著相关，与其他立地因子相关不显著；孢子叶球数量在冠层间的分布呈现由上至下的递减规律，而结实数量在冠层间沿中、上、下递减，结实率沿冠层自上而下递增；孢子叶球与结实数量在树冠不同朝向上也呈现出一定的特点，但总体上差异不显著。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。