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Results:12 strains of streptococcus were isolated from 4 sera and 1 CSF of the infected men,and 7 viscus samples were isolated from pig ill death.

结果 :从 4例病人血液、1例脑脊液和 7例病死猪中分离到 12病原菌,经鉴定 9编码为 0 6 4145 3,2为 0 6 40 45 3,1为 16 4145 3,12菌均与猪链球菌 2型血清凝集。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌的16S rRNA基因序列进行分析,发现其中5细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232), 1细菌属于杆菌目的芽胞杆菌d属(KD178),另外1细菌属于黄色单孢菌目的寡食单胞菌属(KD237);同时我们构建了系统进化树,确定分离菌的相对进化地位。

Methods Two arrays of isolates were used to evaluate the reliability of ChromID ESBL. One array contained 285 clinical isolates in which the ESBLs-producing feature was screened by confirmatory tests recommended by Clinical and laboratory Standards Institute. The other array contained 28 enterobacterial isolates with their ESBLs gene and producing enzyme feature being confirmed previously.

用2组肠杆菌科细菌进行评估,一组为285上海地区检出的临床分离,这些菌已用美国临床实验室标准化研究所推荐的确证试验确认了是否产ESBLs,另一组28产ESBLS肠杆菌科细菌,在以前的研究工作中已确认了这些菌的产酶特性和ESBLs。

Results 159 bacterial strains were isolated. Gram-negative bacilli (101) were 63.52%,fungi (42) were 26.42%;Gram-positive cocci (16) were 10.06%.

结果 分离的159细菌菌,主要以革兰阴性杆菌为主(101)63.52%,真菌(42)26.42%,革兰阳性球菌(16)10.06%。

Results Totally 494 strains of bacteria were isolated, 307 strains of Gram negative bacilli and 187 strains of Gram positive cocci account for 62 1% and 37 9% r...

革兰氏阴性杆菌中铜绿假单胞菌 12 2,肺炎克雷伯氏菌和大肠埃希氏菌 5 2,鲍氏不动杆菌 4 4,嗜麦芽寡养单胞菌 2 7,阴沟肠杆菌 2 3

Gram-negative bacilli (101) were 63.52%,fungi (42) were 26.42%;Gram-positive cocci (16) were 10.06%.

结果 分离的159细菌菌,主要以革兰阴性杆菌为主(101)63.52%,真菌(42)26.42%,革兰阳性球菌(16)10.06%。

Based on the higher alcohols and lactic acid metabolism theory, a method was developed to low higher alcohols capability Saccharomyces cerevisiae strains by screening high lactic acid dehydrogenization enzyme activity in this study.

本研究对Y1110菌及其出发菌、突变等16不同来源的酵母菌进行全基因组RAPD分析,建立并优化了酵母RAPD扩增体系和分型系统,从20条随机引物中筛选出了3条具有较好的扩增多态性,共扩增出34条RAPD谱带,多态性为85.3%;获得了稳定清晰的RAPD指纹图谱。

Results The whole genome of CVB4 jlu06 strain consisted of 7 395 nucleotides encoding 2183 amino acids, which showed high homologies to other CVB4 strains, with the mutations of 10 amino acids. Phylogenetic analysis proved that CVB4 jlu06 strain showed close relationship to HumanCB4 and POLG_CXB4 strains while showed remote relationship to CXB2 strain. Unlike other non-diabetogenic strains, CVB4 jlu06 strain showed common amino acids with diabetogenic strains at 4 sites in 3A region of non-structural protein.

结果 CVB4 jlu06基因组全长7395个核苷酸,编码2183个氨基酸,与其他CVB4的同源性较高,且发生了10个氨基酸的变异,与HumanCB4和POLG_CXB4亲缘关系较近,与CXB2亲缘关系较远;CVB4 jlu06与具有潜在致糖尿病性病毒,在非结构蛋白3A区4个位点有共同的氨基酸,与非致病毒不同。

The serotypes of 16 pathogenic salmonella stains isolated from different swine farms of Sichuan proveince were detected,and there were 6 strains of Salmonella typhimurium,5 strains of Salmonella choleraesuis ,3 strains of Salmonella enteritidis and 2 strains of Salmonella derby.

中文摘要:本研究应用标准单因子血清,对四川不同地区规模化猪场分离的16猪源致病性沙门氏菌进行了血清型鉴定,鉴定出鼠伤寒沙门氏菌(O:1,4,5,12;H1:i;H2:1,2)6、猪霍乱沙门氏菌(O:6,7;H1:c;H2:1,5)5、肠炎沙门氏菌(O:1,9,12;H1:g,m;H2:-)3、德尔俾沙门氏菌(O:1,4,[5],12;H1:f,g;H2:[1,2])2

Analysis is antagonist on the virulence of Erwinia carotovora showed that either the strain culture broth.or the crude enzymes of the strains have the ability to quench the quorum-sensing and attenuate the virulence of Erwinia carotovora.

它们的粗酶液也都具有很强的AHL降解能力,且菌R1的能力要优于菌X1。菌对欧文氏胡萝卜软腐致病菌的拮抗研究表明,无论是菌X1和R1的菌液本身还是菌的粗酶液,对致病菌都具有拮抗作用,都能比较明显的抑制软腐病病害。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

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