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Results Isolated from 500 pneumonic patients were 334 strains of bacteria,of which 73 strains were isolated from patients with the community acquired pneumonia and 265 strains were isolated from pateints with ...

结果500例患者共分离出细菌334株，其中社区获得性肺炎患者分离菌株73株；医院获得性肺炎患者分离菌株265株。

Firstly,the result of the morphological character shows that all strains are short bacillus and gram-negative bacteria except DL7 strain;40%strains have gemma,and the strains which isolated from the root nodule of Podocarpus macrophyllus Thunb.

首先从形态特征的结果来看，所有菌株的菌体均为短杆状，革兰氏染色仅DL7菌株是阳性，其余菌株是阴性，有40%的菌株具有芽孢，其中从罗汉松根瘤分离的菌株形态与根瘤菌的有更多的相似性。

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

A virus strain was isolated from the arthrosis of broiler chickens.The virus was shown to have spherical particles with isometric symmetry,no envelope,double deck capsids,with outer capsid of 75nm,inner one of 50nm in diameter by electron microscopy.The virus was not sensitive to aether,acid and heat.The virus could multiply on the chick embryo fibroblastcell,and could cause syncytial pathologic change with the appearance of cytoplasm inclusion bodies.The challenged chickens of one-day old by nasal,oral,intramuscular and pulvillus injection displayed obvious clinical symptom of severe arthritis and accompanied with pathological changes of malabsorption and necrosis.Meanwhile,four avian reovirus isolates CL,S1133,YH and YB were examined in the cross-neutralization test.

从临床患病鸡关节病料中分离到一株病毒，在电镜下观察到病毒粒子无囊膜，有双层衣壳，外衣壳直径约75nm，内核约50nm；分离病毒株对酸、热有较强的抵抗力，对乙醚不敏感；病毒感染鸡胚成纤维细胞上能产生以融合细胞为特征的CPE；爪垫接种1日龄雏鸡表现出明显的病毒性关节炎临床症状，并伴有吸收障碍型和坏死型的病理变化；血清中和交叉试验表明，该病毒分离株与国内的2株番鸭源呼肠孤病毒分离株和国外S1133呈不同程度的交叉反应。

Different biological and ecological characteristics, virulence, sporulation, conidia bourgeon, Protea-Bse activity leve and colony growth of 6 strains of Beauveria bassiana were measured respectively. The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics. Two strain's have a potential of appling in mass production, BD-B015 and BD-B038 were abtained. The revisory mortality to Laphygma exigua Hubner were 88.33% and 86.70%.The infected rate were 86.67% and 85.27%.

测定初筛获得的6个球孢白僵菌菌株的毒力、产孢量、萌发率、胞外蛋白酶活性及菌株生长速度等多个生物、生态学指标，从专业角度构建最优序列，经灰色分析和不同菌株毒力效价显著性比较，筛选出了两株具有较大生产潜力的优良菌株BD-B015和BD-B038，对甜菜夜蛾的校正死亡率分别为88.33%和86.70%，僵虫率分别为86.67%和85.27%。

Five kinds of bacteria were isolated from disease Meretrix meretrix and culture water (two isolated from the M. meretrix, three from the culture water) in Lvsi harbor, Jiangsu province. The results of infection showed that strain WG1702 can cause disease with the mortality rate 100 percent and the same symptoms as original. It was cued that strain WG1702 was the pathogenic bacteria and caused the death in large scare, a preliminary research was done on the bacteria including morphological, physiological, biochemical characteristics, phylogenetic position and pathogenic. Strain WG1702 was a straight-rod gram negative bacterium, the physiological and biochemical indicators were lysine decarboxylase, ornithine decarboxylase, reduction of NO3- to NO2-, mannitose, oxidase and methylred reaction positive, arginine decarboxylase, arginine dihydrolase, salicin and voges-proskauer negative.

本文从江苏吕泗文蛤养殖区发病文蛤和养殖水体中分离到5株优势菌(病蛤中分离到3株优势菌，养殖水体中分离到2株优势菌)，人工感染实验结果显示，菌株WG1702能引起供试文蛤发病，死亡率为100%，且发病症状与原发病症状相同，提示该菌是引起文蛤大面积死亡的主要致病菌，对其形态、生理生化特征、分类地位及致病性进行了初步研究，菌株WG1702为革兰氏阴性菌，直杆状，生理生化指标为：赖氨酸脱羧酶、鸟氨酸脱羧酶、硝酸盐还原、甘露糖、氧化酶、甲基红阳性，精氨酸脱羧酶、精氨酸双水解酶、水杨素、V.P阴性。

The ratios of the dry weight of stolon and ramet/the dry weight of genet in plot 1 and 5 are the highest, Dry weight of L.

结果表明，除人为干扰比较严重的生境外，基株大小与匍匐茎数量、单位长度匍匐茎干重之间存在极显著的正线形相关（P〈0.01），基株大小对分株的影响可能与匍匐茎数量有关；匍匐茎与分株总干重与基株干重的比值在海拔最高和最低两个样地中最大。

It is indicated that MRV and street viruses have close phylogenic relation by analyzing the phylogenic tree of eachgene of MRV, and it is short evolutionary time between MRV and DRV by analyzingthe phylogenic tree of L gene. It is illuminated that MRV and street virus in China andAfrica have close phylogenic relation by analyzing phylogenic trees of other genes.Therefore, we can speculate that MRV is a Chinese street rabies virus isolated, whichis a new virus isolated because of variance and evolution of the rabies virus after itinfected mouse.

MRV的各个基因进化树表明，其与野毒株具有较近的亲缘关系，通过L基因的进化树分析，两个毒株的进化时间较短，其他基因进化树分析表明，MRV与亚洲和中国流行的野毒株具有较近的亲缘关系，所以推测MRV应该是中国固有的野毒株，由于某种原因感染野鼠后病毒进化变异而产生的毒株。

The results showed that both CDV strains could induce apoptosis of infected cells. However, there was significant difference in the percentage of apoptotic cells between the two strains (P＜0.01). The strain resulted in the CPE type of cell-rounding induced much higher ratio of apoptotic cells than the strain resulted in the CPE type of syncytium.

结果发现，2种毒株感染引起的细胞病变类型不同，分为圆缩型与合胞体型；2种毒株均可诱导感染细胞凋亡，但毒株间的凋亡细胞比例存在差异，即产生合胞体型细胞病变的毒株凋亡出现较晚，凋亡细胞比例显著低于产生圆缩型细胞病变的毒株(P＜0.01)。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法，结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察，观察结果表明：不育株与可育株在花粉母细胞时期差别不大，不育株的花药小孢子母细胞进入减数分裂期后，可以正常的形成四分体，四分体前期发育正常，而在单核小孢子期绒毡层细胞出现液泡化，绒毡层细胞向中间挤压小孢子。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。