株

Kala-azarpatients were examined by ELISA with three Leishmania strains antigen, the results were 901 strain with 80.92%, 801 strain with 92.25% and 951 strain with 89.92%. The result was notable difference between 901 strain and 801 strain. 334 healthy persons serum of endemic areas were tested by ELISA with three Leishmania strains antigen, the positive rates were notable differenceamong three antigens.

结果：提取的三株抗原的蛋白浓度分别为：901株1000ug/ml、801株800ug/ml、951株1000ug/ml；工作浓度均为10ug/ml；三株抗原分别检测24份肺结核病人血清，均为阴性；三株抗原分别检测60份非疫区健康人血清，801株与951株的检测结果均为阴性，901株检测出一份阳性；三株抗原分别检测131份黑热病病人血清，阳性率分别为：901株80.92%、801株92.25%、951株89.92%。801株明显高于901株。

Results The distinctive PCR products of the 6 isolated strains from Liaoning and Jilin areas through restriction with Hinf I ,Hha I ,Rsa I appeared two different kinds of RFLP restriction map. The strains HCAa9202,HCAp9203 and HCM9501 had the similar restriction maps with reference strain Gilliam,but they did not have restriction site of Hinf I ;Strains KDCt9402,KDCp9403 and KDCt9404 had the simular testriction map with reference strain Karp.

结果辽宁、吉林地区6株分离株的PGR特异性产物经Hinf I、Hha I、Rsa I酶切后呈现2种不同的RFLP图谱：HCAa9202株、HCAp9203株及HCM9501株与Gilliam参考株具有相似的酶切图谱，但缺乏Hinf I的酶切位点；KDCt9402株、KDCp9403株及KDCt9404株与Karp参考株具有相同的酶切图谱。

Four hundred and twenty three ( 262 for HB×T, 49 for S×T, 111for R×T and 1 for B×T) out of 990 individuals exhibiting the trifoliate phenotype oftrifoliate orange were considered to be hybrids by morphology identification and SSRverification; Six hundred and fourteen of 756 plantlets derived from other crosses wereverified to be hybrids by SSR analysis. Of them, 244 for HB×\'Nova\' tangelo, 179 forHB×\'Page\' tangelo, 172 for HB×\'Fairchild\' tangelo, 1 for \'Bendizao\'tangerine×\'Nova\'tangelo, 7 for \'Bendizao\'tangerine×\'Page\' tangelo, 3 for \'Bendizao\'tangerine×\'Murcott\'tangor and 8 for satsuma mandarin×\'Nova\' tangelo.

为区分杂种苗和珠心苗，用形态识别和SSR分析，鉴定了990株枳的杂交后代，得到了423株三叶杂种(HB柚×枳262株，温州蜜柑×枳49株，红橘×枳111株，华农本地早橘×枳1株)；SSR分析鉴定了其它杂交后代的756个单株，结果共获得了614株杂种，其中HB柚×诺瓦244株，HB柚×佩奇179株，HB柚×费尔切尔德172株，华农本地早橘×诺瓦1株，华农本地早橘×佩奇7株，华农本地早橘×默科特3株，温州蜜柑×诺瓦8株。

The cultural condition of PGPR strains Temperature,pH,light,cultural method,acid or alkali producing,and salt tolerance of 9 PGPR strains isolated from Gramineous Forage were tested,the results showed that all the PGPR strains could grow in the temperature range from 5℃～45℃,and optimum temperature for 178,O-6,Dry6 strains was 35℃,for X5,173,Y5,C-6 strains was 30℃,for N4 strain was 25℃～35℃,for 86 strain was 25℃;most of PGPR strains prefered to neutral or alkaline condition,strains 178,O-6,N4 and X5 were preferable to alkali condition especially;light was beneficial to PGPR's growth;all of them produce alkali;most of PGPR strains were not sensitive to NaCl concentration;all the strains were aerobiotic bacteria.

结果表明：各供试菌株对温度的适应范围较广，在5℃～45℃范围内均能生长，178、O-6、Dry6 菌株的适宜生长温度为35℃，X5、173、Y5、C-6 菌株的适宜生长温度为30℃，N4 菌株的适宜生长温度为25℃～35℃，86 菌株的适宜生长温度为25℃；大部分菌株在中性或偏碱性的条件下生长好，特别是Dry6、N4 和X5 菌株对碱性环境适应性强，在pH 值8.0 时生长最好；光照有利于菌株的生长；绝大部分菌株在3%NaCl浓度下生长良好，在5%～7% NaCl 浓度下除173 和86 菌株外，其它菌株都能生长，即对盐份的耐受性较好，且9 个菌株均为产碱菌；除173 菌株是兼性厌氧细菌外，其它都是好氧性细菌。

Enteritidis were obtained from poultry and its meat products.The antibiotic resistance of food isolates was higher than clinical isolates. The drug resistance spectrum of diarrhea patients isolates in 2006 was different from that in 2007.76 strains including 13 food-borne strains belong to 21 PFGE pattern combinations,and their genetic similarly was higher than 80%.

包括13株食品株在内共76株肠炎菌株可分为21种PFGE带型，遗传同源性接近80%，优势带型依次为3型（34株）、1型（12株）、2型（5株）和5型（5株），除2型外均发现有与腹泻株同型的食源株。2006和2007年的PFGE1/2型病例分别为4例和12例。

From 94 soil samples and 9 bark samples in these areas, 259 Myxobacteria strains were isolated and 140 strains of them were purified. These 259 Myxobacteria strains were primarily identified in genus or species level by their fruiting bodies, myxospores and vegetative cells on the base of Sergey's manual of determinative bacteriology and they belonged to 9 genera(Myxococcus, Corallococcm. Angiococcus. Archangium, Cystobacter. Stigmatella . Hapoangium. Nannocystis. Polyangium) of Myxococcales.

根据菌株的特征性子实体形态和其营养细胞、粘孢子形态，以《Bergey's manualof determinative bacteriology》第九版为依据，将分离纯化出的菌株初步鉴定到属，这259株粘细菌菌株经过初步的分类鉴定，分属于9个属，即粘球菌属139株、珊瑚球菌属20株、囊球菌属18株、原囊菌属21株、孢囊杆菌属5株、标桩菌属36株、单囊菌属9株、小囊菌属3株，和多囊菌属8株。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究，於宜兰五结垃圾场和台中市环保局厨余堆肥样品中共分离出 23 株具脂肪酶活性菌株， 23 株菌株中经过三油酸甘油酯的洋菜培养基於 50℃培养 5 天后，发现有 2 株菌的脂肪酶活性较强，分别为分离株 B61 和 M63；以本实验室先前自台大女九餐厅蔬果厨余堆肥中所筛选出的耐高温脂解菌 Brevibacillus borstelensis SH168作为本实验对照菌株，将分离株 B61、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液态培养基培养并测定其脂肪酶活性，发现分离菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，认为分离株 B61、M63 适於产生脂肪酶的菌株，分离株 B61 在 pH 5~9 皆可生长，生长温度介於 20 oC ~ 60oC；分离株 M63 在 pH 6~10 可生长，生长温度介於 30 oC ~ 50oC； 2 株分离菌株以 16S rRNA定序结果分离株 B61 为 Bacillus circulans，分离株 M63 为 Bacillus 属。

A total of 200 strains (z50% gentamicinresistant) from the United States were used for the cross-resistance study including Staphylococcus aureus (110), coagulase-negative staphylococci (CoNS; 50), Pseudomonas aeruginosa (10), Escherichia coli (20), and other Enterobacteriaceae (10) tested against TAO, bacitracin, polymyxin B, neomycin, amikacin, gentamicin, streptomycin, tobramycin, and mupirocin. Fifty gentamicin-resistant isolates from each year (1997–2002) were used to determine the activity of TAO over time. Baseline resistance rates of TAO among 300 Australian isolates (AGARS Program, 2002–2003) were also studied.

来至美国的200株菌株（ z50 ％对庆大霉素有耐药性）用于交叉耐药性研究，其中包括金黄色葡萄球菌（ 110株），凝固酶阴性葡萄球菌（ CoNS ； 50株），铜绿假单胞菌（ 10株），大肠埃希氏菌（ 20 株）和其他肠杆菌属菌（ 10株），检测这些菌对于以下抗生素的耐药性，抗生素为TAO，杆菌肽，多粘菌素B ，新霉素，丁胺卡那霉素，庆大霉素，链霉素，妥布霉素，莫匹罗星。1997到2002年期间每年分离出五十株对庆大霉素有抗药性菌株来确定随时间的变化TAO对其的活性是否有所改变。300株澳大利亚分离的菌株(AGARS Program， 2002–2003)也对TAO的耐药性基准进行了研究。

Separated cultures were isolated from 4 different koumiss examples, the complete 16SrRNA gene of these strains were sequenced, and phylogenetic trees were constructed. The isolateswere identified to the most related strain which had the highest affinity. The identified strains weresix L. lactis, five L. plantarum, five E. faecium, four E. faecalis, two L. casei, two L. helveticus, twoS. thermophilus, one L. delbrueckii subsp, bulgaricus, one Leu. mesenteroides, one Leu. garlicum.

本研究中从4份酸马奶中分离得到了29株乳酸菌，测定了这些乳酸菌的16S rRNA基因全长序列，并构建了乳酸菌属的系统发育树，根据亲缘关系对乳酸菌进行了鉴定，其中L.lactis(6株)，L.plantarum(5株)，E.faecium(5株)，E.faecalis(4株)，L.casei(2株)，L.helveticus(2株)，S.thermophilus(2株)，L.delbrueckii subsp.bulgaricus(1株)，Leu.mesenteroides(1株)，Leu.garlicum(1株)。

In conclusion, the mutations of rpoB gene in this research are consistent to the other research repots from internal and external coutry.

与DNA测序结果比较，168株中有164株被判定为耐药株，4株判定为敏感株；132株测序未发现突变的菌株中129株被判定为敏感株，3株被判定为耐药株。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。