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METHODS: Rat models of focal cerebral ischemia were established by Zea Longa suture method in each group.

各组大鼠均采用Zea Longa线栓法建立局灶性脑缺血模型。

Methods The middle cerebral artery was occluded for different time with modified Zea Longa,s MCAO model.

采用改良Zea Longa线栓法大鼠MCAO模型，将大脑中动脉闭塞不同的时间。

The model of MCAO was made by occluding the MCA with thread according to Longa＇ s method.

采用Longa线栓法制成年sD大鼠大脑中动脉闭塞模型。

The middle cerebral artery occlusion/reperfusion rat model was established by the Longa occlusion method

参照Longa线栓法制作大脑中动脉阻塞再灌注动物模型

Methods32 Wistar male rats were used to induce focal cerebral ischemia according to Longa's method.

3 2只Wistar雄性大鼠，按照Longa的线栓法制作局部脑缺血模型，但在放入栓塞线时不结扎翼腭动脉，通过局部脑血流监测、动物行为学评分、脑含水量测定、红四氮唑染色和病理学检查等方法评价该模型的可靠性。

Methods: The string inserting method according to Longa＇s report was used to induce the model of rats with focal cerebral ischemia/reperfusion.

采用Longa报道的线栓法制备大鼠局灶性脑缺血-再灌注模型。

Staphylokinase is a 136-amino acid protein,secreted by certain strains of lysogenesis Staphylococci with thrombolytic potential.

葡激酶是由溶原性葡萄球菌分泌的一种具有潜在溶栓功能的含有136个氨基酸的蛋白质。

Studies show IV lytic therapy has decreased to 15% and primary PCI has increased in the past 5 years to 65%.

研究发现，在过去的5年中，接受静脉溶栓治疗的患者下降到15%，而接受急诊PCI的患者增加到现在的65%。

INTERVENTION: The models of ischemia/reperfusion in SD rats were established by middle cerebral artery occlusion with a nylon monofilament suture. The rats were randomly divided into the treatment groups 32cases and the control group 32 cases. Each group was divided into eight subgroups, which consisted of 4 rats at 2 hours, 6 hours, 12 hours, 24 hours, 2days , 3 days, 7 days and 14 days after reperfusion of MACO.

干预措施：成年健康雌性SD大鼠68只，应用线栓法建立SD大鼠大脑中动脉阻塞再灌注模型，随机分为治疗组32只和对照组32只，每组再随机分为缺血1.5 h再灌流2，6，12，24 h，2，3，7，14 d组(n=4)，另外4只作假手术组。

In the experiment, depended on the traditional ischemia model MCAO, we both use the clot embolus model to study the edema of the neuron cell. We found that the hyperintense signal was seen in the DWI in few minutes by using the MACO models after the ischemia and reperfusion.

本实验在利用传统缺血模型MCAO基础上，结合与人类缺血卒中病程较接近的自体血栓脑栓塞模型，模拟急性脑卒中的过程，研究急性期缺血后神经细胞水肿发展的规律，了解神经元的生物代谢物质变化趋势，评价早期溶栓治疗的实验意义。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。