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The amino acids sequences alignment confirmed that 145 LRR stucture was highly conserved in all aligned PGIP sequences. The results of phylogenetic analysis showed that BoPGIP1 and PGIP from Brassica napus and Arabidopsis thaliana clustered together firstly, and then came from Gossypium barbadense, Lycopersicon esculentum and Phaseolus vulgaris in turn. The clustering results basically accorded with morphological classification system.

将BoPGIP1氨基酸序列与数据库中的其他植物PGIP进行同源序列比对,构建系统进化,发现所比对基因都含有LRR结构,在145位点的LRR结构域在所有物种中极其保守;系统显示,该序列最先与甘蓝型油菜和拟南芥聚类,其次和棉花、番茄和豆科作物的PGIP聚类,基本符合按形态特征分类的亲缘关系。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001~0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点,远多于S群体(14)和G(21)群体,而且在53个特有位点中有9个出现频率为100%的位点,这些位点可以作为区分其它2个群体的特征性标记;S– G群体特有的位点有112个,其中有4个位点出现频率为100%,可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585,遗传距离只有0.0424,在NJ和UPGMA法构建的亲缘关系的状图上均首先聚在一起,说明二者的亲缘关系很近,应属于种内群体间的关系;而W与S和G的遗传相似性系数均较小(0.7939和0.7941),相对遗传距离很大而且十分相近(0.2308和0.2305),在亲缘关系状图上单独分出一支,也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现,W的ITS序列长度在1266-1269 bp,而S和与G的ITS序列总长度分别为1520 bp和1614 bp;从ITS1和ITS2长度来看,W分别为739-741 bp和316-317 bp,S为895 bp和414 bp,G为987 bp和416 bp;而从ITS碱基组成来看,W的GC含量在62.32-62.62%之间,而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003,S与G群体间的遗传距离是0.010,说明W群体内变异很小,而S与G群体间已出现明显的遗传分化,但还均属于种内群体间的遗传变异;而W与G和S的遗传距离分别达到0.110、0.147,两个类群差异显著,已远超出种内群体间的遗传变异。

Byatt,Doris lessing,Zadie Smity,Victoria Hislop,Martin Amis, Minette Walters; USA writers Irwin Shaw,Jodi Picoult,Truman Capote,ToniMorrison,Karolyn Parkhurst;Japanese writer Yamaoka Sohachi, Tanaka Yoshiki,Kuroyanagi Tetsuko,Kawakami Hiromi,Matsumoto Seicho,Miyabe Miyuki, Akagawa Jirou,Higashino Keigo, Murakami Haruki,Shimada Yoshichi;Brazil writer Paulo Coelho and so on.

拜亚特、多丽丝·莱辛、查蒂·史密斯、维多利亚·希丝洛普、马丁·阿米斯、米涅·渥特丝;美国作家欧文·肖,杜鲁门·卡波特,托妮·莫里森,牙买加·琴凯德,朱迪·皮考特,卡罗琳·帕克丝特等;日本作家山冈庄八,田中芳,黑柳彻子,川上弘美,松本清张,宫部美雪,赤川次郎,东野圭吾,村上春,岛田洋七;巴西作家保罗·柯艾略等。

" Preparation of Jatropha Curcas Protein Concentrate and Seed Meal Free of Antinutrients and Toxic Factors, and Their Utilization in Monogastric, Fish and Ruminant Nutrition", funded by Ministry of Science and Technology, China.

麻疯脱毒浓缩蛋白与饼粕开发及其在养殖业中的应用,科技部国际合作重点项目,主要研究麻疯籽实油粕脱毒技术以及在脱毒饼粕在畜牧生产上的应用效果评价。

With three times the protein of beef, mopane caterpillars are a favorite food in southern Africa. Some cook them in a stew with tomatoes and onions, and some eat them dried as a snack. The worms must be harvested from the mopane trees, cooked and dried in the sun. Then they are ready to enjoy.

莫培尼毛毛虫(译注:Imbrasia Belina蛾的幼虫)则是非洲南部受欢迎的佳肴,有些人喜欢把这种毛毛虫加进番茄、洋葱一起炖煮,有些人则偏好嚼虫乾当点心吃,只有莫培尼才能捕获这种毛毛虫,煮熟後再曝晒在阳光下,然後就可供享用了。

With three times the protein of beef, mopane caterpillars are a favorite food in southern Africa. Some cook them in a stew with tomatoes and onions, and some eat them dried as a snack. The worms must be harvested from the mopane trees, cooked and dried in the sun. Then they are ready to enjoy.

莫培尼毛毛虫(译注:Imbrasia Belina蛾的幼虫)则是非洲南部受欢迎的佳肴,有些人喜欢把这种毛毛虫加进番茄、洋葱一起炖煮,有些人则偏好嚼虫乾当点心吃,只有莫培尼才能捕获这种毛毛虫,煮熟后再曝晒在阳光下,然后就可供享用了。

The wind came a faint smell, my breath to bypass efforts to find late spring, Muran Looking back, I realized that the hot Huacheng its presence everywhere, it is difficult to see the banyan tree, Zhejiang, is the year In early summer, stubborn, I must go to satisfy the wish of a child pulling you go to children's playground, and then I saw the lake Nazhu banyan tree, I cling to tree trunks Wawadajiao that two of us do not live so sure that encircle thick trees, so you come to the tree on the other side, holding my hand, trying to pull the other.

风里传来一阵淡淡的气味,我绕过晚春的气息努力寻找,暮然回首,才发现原来炎热的花城到处都是它的身影,那是浙江很难见到的榕,是那一年的初夏,固执的我一定要去满足儿时的心愿,拉着你去儿童乐园,然后我看到了湖边的那株大榕,我抱住干哇哇大叫,说我们两个人肯定合抱不住这么粗的,于是你走到的另一边,牵着我的一只手,尽力去拉另一只。

Observe the influence of combination therapy with microwave ablationand peritumoral injection of immature dendritic cells on survival and tumor growth in mice.Observe clinical safety of combination therapy with MWA and adoptive immunotherapy of dendritic cells and eJector cells.Research the change of percentage of lymphocyte subsets and concentration of cytokine in peripheral blood after combination therapy.Initially observe the short-term result of combination therapy.

目的摸索微波消融治疗时有效灭活肿瘤细胞并释放肿瘤抗原的温控和能量条件;观察肝癌消融后局部注射未成熟突状细胞对荷瘤小鼠生存期及肿瘤生长的影响;观察肝癌微波消融联合突状细胞疫苗及效应细胞综合过继免疫治疗的临床安全性,研究微波联合细胞免疫治疗后近期外周血淋巴亚群比例及细胞因子浓度的变化,初步探讨细胞免疫治疗对肝癌微波消融后近期复发率的影响。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件,消融人肝细胞癌株SMMC-7721细胞悬液,分为五组:A、对照组,B、45℃10min组,C、50℃3min组,D、54℃3min组,E、60℃即刻组,消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤,间隔1周,右侧腹股沟皮下接种瘤块作为观察瘤,成瘤后分为四组:A组,对照组,不行治疗;B组,微波组,仅微波消融小鼠消融瘤;C组,成熟DC组,微波消融小鼠消融瘤,并于小鼠双侧腋下注射肿瘤抗原致敏的突状细胞;D组,未成熟DC组,微波消融小鼠消融瘤,并于消融瘤周注射未成熟突状细胞。

Speak, he pointed to the yard was chik winding Zhuge Liang of a tree to see:"Why do you look at the tree died Nake Not life and death do not live, do not go long?"

说着,他指着庭院里被葛藤缠绕的一棵让诸葛亮看:&你看那棵为啥死不死活不活,不往上长呢?&

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。