标记

Background and Objective The goals of this study were to label the antisense oligodeoxynucleotidesindirectly by Chloramine Tmethod and Iodogen method,then to discuss the labeling yields、radiochemical purity、specific activity and stability in different conditions.

目的与研究背景：本课题采用氯胺-T法和Iodogen法两种标记方法进行反义寡脱氧核苷酸的125I间接标记对比研究，并在两种方法中分别探讨不同反应条件下的标记率及标记产物的放化纯、比活度和稳定性。

Background and Objective The goals of this study were to label the antisense oligodeoxynucleotides indirectly by Chloramine T method and Iodogen method, then to discuss the labeling yields、radiochemical purity、specific activity and stability in different conditions. We discussed the right labeling method and conditions in order to lay the experiment foundation of the antisense medicament labeled by iodine for clinical application.

目的与研究背景：本课题采用氯胺-T法和Iodogen法两种标记方法进行反义寡脱氧核苷酸的125I间接标记对比研究，并在两种方法中分别探讨不同反应条件下的标记率及标记产物的放化纯、比活度和稳定性。

This paper discusses three Chinese marked constructions: address,circumbendibus and focus , focus ing on their social factors ,based on social cognition.

文章分析汉语中的三类有标记构式：称谓类有标记构式、委婉类有标记构式、焦点类有标记构式，重点考察它们的一部分小类以及与之相关的社会因素。

This paper discusses three Chinese marked constructions: address, circumbendibus and focus, focusing on their social factors ,based on social cognition.

文章分析汉语中的三类有标记构式：称谓类有标记构式、委婉类有标记构式、焦点类有标记构式，重点考察它们的一部分小类以及与之相关的社会因素。

Said cell resorting method includes using sorting label generating module inserting time mark and serial number mark into cell, then transmitting cell from source port to target port; resorting module according to received cell carried time mark and serial number mark resorting cell enqueue information.

该信元重排序方法包括以下步骤：使用排序标签生成模块将时间标记和序号标记插入信元，然后将信元从源端口发送到目的端口；重排序模块根据在目的端口接收到的信元携带的时间标记和序号标记，重排序信元的入队信息。

Prussian blue staining revealed more than 98% of BM-MNCs were labeled by Feridex and BM-MNCs appeared to be unaffected by magnetically labeling. Three dogs injected Feridex- BM-MNCs were visualized at one week by MRI, but none of unlabeled BM-MNCs were seen. All lesions that were detected within one week could not be visualized over four week. The dot injected Feridex-cells which fixated by 4 % methanal also was seen at one week by MRI, but the signal disappeared at two week.

普鲁士蓝染色显示体外Feridex标记BM-MNCs成功率达98%以上，而且细胞活性不受影响；其中3只实验犬标记细胞移植区1周内可用1.5T磁共振仪3DSSFP序列在体扫描到T2低信号影像，4周时该低信号消失；同样条件下经甲醛固定的标记细胞在1周时也可检测到T2低信号，但2周时该低信号消失；未标记细胞移植区没有检测到低信号。

Genetic cluster analysis is similar based on the results of morphological and DNA markers, the five populations could be divided into three groups. But there are different groups sort of individual locations, reflecting the similarities and difference about the analysis of the relationship between population with the two kinds of markers, It could be concluded that there was coupling and relevance to some extent in evaluating genetic diversity with the same experiment sampling by morphological and SSR marker variation. However, there was also differences. In research, the two can complement each other.8. Identification of Genetic Relationship of Amygdalus plants by SSRThe genetic relationship among 55 Amygdalus plants collections, belonging to 6 species and collected from both homeland and abroad were identified via SSR molecular marker technique.

新疆野扁桃遗传多样性不同研究分析方法结果的比较与评价表型与DNA二种水平的遗传标记方法揭示的遗传多样性水平有差异，表型性状比DNA分子标记具有更大的变异性；其受到环境的影响较大，也即对环境更敏感。2种水平的遗传标记聚类结果将野扁桃5个不同分布群体均可分为3个大类群，但是出现有个别不同的群体排序位置，反映了2种标记在分析种群间关系上的异同性，从本研究结果来看，说明表型与DNA标记有一定的耦合性和相关性，但也存在差异，在研究上可以互为补充。8。

The principles of several DNA Molecular markers and their application on assessment of germplasm genetic diversity, fingerprinting construction, classification of heterotic groups and heterosis prediction for maize were reviewed.

DNA Molecular markers ； DNA分子标记是继形态标记、细胞标记和生化标记之后发展起来的一类重要的遗传标记，已在生命科学领域中广泛应用。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Among the four characteristic bands,air-cured tobacco including maryland tobacco and Burley tobacco,sun cured tobacco including oriental tobacco and sun-cured tobacco,and flue-cured tobacco and air-cured tobacco were distinguished.

两条晾烟类型的特征标记带、一条晒烟类型的特征标记带，一条烤烟与晾烟特征标记带。这些标记不但可以直接转化成SCAR标记，而且可通过分离群体的遗传分析用于烟草类型间遗传转化的特征带，也可进一步用于测序发掘新基因。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。