标记

After that, all samples were fixed with 10 g/L paraform for 45 minutes. Then 50 μL well-fixed blood was added into the polystyrene tube, meanwhile 20 μL monoclonal antibody, such as CD42a-b-c-d and PE-labeled CD42b, was respectively mixed gently with the blood sample and incubated at room temperature in dark for 30 minutes.

取50 μL固定后全血加至流式检测管，同时分别向不同管中加入20 μL糖蛋白Ib/IX/V 复合物单克隆抗体CD42a-b-c-d和PE标记的糖蛋白Ibα单克隆抗体CD42b，混匀后室温避光孵育30 min，然后CD42a-b-c-d标记的样品中加入20 μL FITC标记的羊抗鼠 IgG，混匀后室温避光孵育30 min。

With these 2 elements mark is opposite 10 China sweet potato advocate grow breed to undertake detecting, earnings result and result of groovy method appraisal agree completely, show 2 elements mark can be used at sweet potato to fight mark of cauline wireworm ill element to assist breeding.

用这2个分子标记对10个中国甘薯主栽品种进行检测，所得结果和常规方法鉴定结果完全一致，表明2个分子标记可用于甘薯抗茎线虫病分子标记辅助育种。

CLIA using acridinium ester derivatives as chemiluminescent label has advantages of low background,high signal-to-noise ratio,no need of catalyst and simple luminescence system.

以吖啶酯衍生物为发光标记物的CLIA的特点是：标记简单、标记物稳定、不需要催化剂、自然本底低、受外界干扰因素少、信噪比高、发光体系简单等。

The detectable group, biotin-ε-aminocaproic acid N-hydroxysuccinimide ester, is attached to the remaining amino group. A series of multiamine compouds, i. e. ethylendiamine, 1, 6-hexandediamine, triethylenetetramine, tetraethylenepentamine, and synthetic polyarylamide are used inlabelling, it is found that linker arms and steric hindrance effect the detection sensitivity. The probes labeled by 1, 6-hexandediamine and triethylenetetramine can detect 0.1-0.5pg target DNA in dot blot hybridization, and their sensitivities are better than the others.

本文详细考察了乙二胺、己二胺、二乙烯三胺、三乙烯四胺、四乙烯五胺及合成的聚乙烯胺化合物对λDNA探针的标记和检测的影响，发现胺化物链长和空间位阻影响探针的杂交和检测，用己二胺和三乙烯四胺标记的生物素探针在杂交24小时、显色8小时后检测灵敏度达0.1-0.5pg，超过其它胺化物标记的探针。

Because of its apomictic reproduction character, an accurate and rapid identification of the F_1 hybrid seedlings of Malus xiaojinensis is of great importance to both genetic study and utilization of the precious genes in the germplasm.

为了建立用现代分子标记鉴定苹果杂种实生苗的方法，在本论文中我们对小金海棠和山荆子F_1代杂交实生苗79个单株进行了RAPD分析，并用SSR标记对RAPD标记的初步鉴定结果进行了进一步的验证。

If a user operates an operation panel to input an instruction for normal playback, then a control circuit of a digital television monitor determines a frame to be played back at present as a noticed frame and requests the hard disk recorder for image data of successive 15 frames forwardly and backwardly adjacent the noticed frame and image data at positions of time intervals of 0.5 seconds within a range of 2 minutes forwardly and backwardly of the noticed frame through an IEEE 1394 cable.

如果用户操作操作面板输入正常播放指令，那么数字电视监视器的控制电路确定当前播放的一帧作为标记帧，并且通过IEEE1394电缆向硬盘记录器请求标记帧的向前和向后连续的15帧的图像数据以及标记帧向前和向后2分钟范围内在0.5秒时隙内的位置的图像数据。

Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager 2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating populations.

利用SSR分子标记结合&拟测交&策略，以荷包红鲤抗寒品系和柏氏鲤远缘杂交所产生F1代为亲本及其F2代为作图群体，应用Windows Map Manager 2.0软件的标记回归法进行乳酸脱氢酶(Lactate dehydrogenase， LDH)活性数量性状基因座单标记定位分析。

Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating popula-tions.

利用SSR分子标记结合&拟测交&策略，以荷包红鲤抗寒品系和柏氏鲤远缘杂交所产生F1代为亲本及其F2代为作图群体，应用Windows Map Manager 2.0软件的标记回归法进行乳酸脱氢酶(Lactate dehydrogenase， LDH)活性数量性状基因座单标记定位分析。

According to the anti-counterfeiting laser molded holographic imaging technology identifies the hologram of different can be divided into three dimensional laser pulse classics, bolographic anti-false and molded synthetic molding, multiple image laser molding, dynamic molding, dynamic grating molding, embedded hologram molded, inks encryption such as holographic laser molded anti-false identification.

根据激平模压全息不攻假标记全息图不败像技艺的差别可不兵其不合为经典、脉冲不一维激平模压全息不攻假标记以及不合不败模压、众轻图像激平模压、不静态模压、不静态平栅模压、隐不神全息模压、不攻假油墨加密激平模压等全息不攻假标记。

Eighteen (75%) trees were marked with secretion of the crissal gland at an average height of 51.8±15.6cm, and the remaining six were marked with urine at an average height of 64.5±14.5cm.

嗅味标记中，75%的嗅味标记为肛周腺分泌物，标记中心的平均高度为51.8±15.6cm(N=18)，25%为尿液，尿液上沿的平均高度为64.5±14.5cm(N=6)。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。